140346

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 262861..263387 Replicon chromosome
Accession NZ_CP044152
Organism Shigella flexneri strain AR-0425

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag F4V37_RS01340 Protein ID WP_000323025.1
Coordinates 263100..263387 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag F4V37_RS01335 Protein ID WP_000534858.1
Coordinates 262861..263100 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F4V37_RS01310 (258509) 258509..258712 + 204 WP_000214712.1 putative selenium delivery protein YdfZ -
F4V37_RS01315 (258748) 258748..260208 - 1461 WP_000527804.1 mannitol dehydrogenase family protein -
F4V37_RS01320 (260297) 260297..261316 - 1020 Protein_257 MHS family MFS transporter YdfJ -
F4V37_RS01325 (261378) 261378..262534 + 1157 Protein_258 IS3 family transposase -
F4V37_RS01330 (262531) 262531..262836 - 306 WP_071818640.1 hypothetical protein -
F4V37_RS01335 (262861) 262861..263100 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
F4V37_RS01340 (263100) 263100..263387 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
F4V37_RS01345 (263459) 263459..263614 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
F4V37_RS01350 (263832) 263832..264083 + 252 WP_000980987.1 protein Rem -
F4V37_RS01355 (264150) 264150..264428 + 279 Protein_264 hypothetical protein -
F4V37_RS01360 (264430) 264430..265479 + 1050 WP_001265249.1 DUF968 domain-containing protein -
F4V37_RS01365 (265492) 265492..265848 + 357 WP_000904111.1 RusA family crossover junction endodeoxyribonuclease -
F4V37_RS01370 (265863) 265863..266684 + 822 WP_000762882.1 antitermination protein -
F4V37_RS01390 (267604) 267604..267712 + 109 Protein_268 DUF3927 family protein -
F4V37_RS01395 (267993) 267993..268328 - 336 WP_000871291.1 anti-adapter protein IraM -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 260297..276645 16348


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T140346 WP_000323025.1 NZ_CP044152:263100-263387 [Shigella flexneri]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T140346 NZ_CP059122:6870-7124 [Escherichia coli]
ATGAGAAAAAACAAAGGGCGATTGACTTATTATCTTGAAGTGATTGATAAAAAATACCATTTTGTAAAAAAAATAAGCAG
TTATTCAAAGGAGTTCACTGACGGAAAAACAAAAAGAACAAAGAGAACGTTAAGTGAGCTGGTTTTTAATGAAAGTGAGG
TCGAGGCAATAGACTTTACAAAAAATGGTTTAAGACCTGTTGATAAGAATATTCTCTTAACTATGGTGAAAGAATATAAG
GAGAGTGATGCATGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT140346 WP_000534858.1 NZ_CP044152:262861-263100 [Shigella flexneri]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT140346 NZ_CP059122:7121-7363 [Escherichia coli]
ATGACTATTGAAAATACACCGGAAAACATAAAGAAATTACGAAAAAAAATTGGCCTTACCCAGACAGAGTGCGGTGAGAT
TTTTGGCGTAGGCTTAAGTACATGGCAGAAAAAAGAAGCTAAAACCCACAACCAGCTTAATTTATCGAAAGGTGAATTTG
AGTACCTGTTATTACTTGCAGGGGAGCACCCTGAATACGTCCTGCATAAAAAAGCTGAATCCCGCTCACCGGAGGAACCG
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CTS8

References