Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 262861..263387 Replicon chromosome
Accession NZ_CP044152
Organism Shigella flexneri strain AR-0425

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag F4V37_RS01340 Protein ID WP_000323025.1
Coordinates 263100..263387 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag F4V37_RS01335 Protein ID WP_000534858.1
Coordinates 262861..263100 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F4V37_RS01310 (258509) 258509..258712 + 204 WP_000214712.1 putative selenium delivery protein YdfZ -
F4V37_RS01315 (258748) 258748..260208 - 1461 WP_000527804.1 mannitol dehydrogenase family protein -
F4V37_RS01320 (260297) 260297..261316 - 1020 Protein_257 MHS family MFS transporter YdfJ -
F4V37_RS01325 (261378) 261378..262534 + 1157 Protein_258 IS3 family transposase -
F4V37_RS01330 (262531) 262531..262836 - 306 WP_071818640.1 hypothetical protein -
F4V37_RS01335 (262861) 262861..263100 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
F4V37_RS01340 (263100) 263100..263387 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
F4V37_RS01345 (263459) 263459..263614 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
F4V37_RS01350 (263832) 263832..264083 + 252 WP_000980987.1 protein Rem -
F4V37_RS01355 (264150) 264150..264428 + 279 Protein_264 hypothetical protein -
F4V37_RS01360 (264430) 264430..265479 + 1050 WP_001265249.1 DUF968 domain-containing protein -
F4V37_RS01365 (265492) 265492..265848 + 357 WP_000904111.1 RusA family crossover junction endodeoxyribonuclease -
F4V37_RS01370 (265863) 265863..266684 + 822 WP_000762882.1 antitermination protein -
F4V37_RS01390 (267604) 267604..267712 + 109 Protein_268 DUF3927 family protein -
F4V37_RS01395 (267993) 267993..268328 - 336 WP_000871291.1 anti-adapter protein IraM -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 260297..276645 16348


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T140346 WP_000323025.1 NZ_CP044152:263100-263387 [Shigella flexneri]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T140346 NZ_CP059122:6870-7124 [Escherichia coli]
ATGAGAAAAAACAAAGGGCGATTGACTTATTATCTTGAAGTGATTGATAAAAAATACCATTTTGTAAAAAAAATAAGCAG
TTATTCAAAGGAGTTCACTGACGGAAAAACAAAAAGAACAAAGAGAACGTTAAGTGAGCTGGTTTTTAATGAAAGTGAGG
TCGAGGCAATAGACTTTACAAAAAATGGTTTAAGACCTGTTGATAAGAATATTCTCTTAACTATGGTGAAAGAATATAAG
GAGAGTGATGCATGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT140346 WP_000534858.1 NZ_CP044152:262861-263100 [Shigella flexneri]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT140346 NZ_CP059122:7121-7363 [Escherichia coli]
ATGACTATTGAAAATACACCGGAAAACATAAAGAAATTACGAAAAAAAATTGGCCTTACCCAGACAGAGTGCGGTGAGAT
TTTTGGCGTAGGCTTAAGTACATGGCAGAAAAAAGAAGCTAAAACCCACAACCAGCTTAATTTATCGAAAGGTGAATTTG
AGTACCTGTTATTACTTGCAGGGGAGCACCCTGAATACGTCCTGCATAAAAAAGCTGAATCCCGCTCACCGGAGGAACCG
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References