Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 3912385..3912911 Replicon chromosome
Accession NZ_CP012735
Organism Shigella flexneri 1a strain 0228

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag AOT98_RS21500 Protein ID WP_000323025.1
Coordinates 3912385..3912672 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag AOT98_RS21505 Protein ID WP_000534858.1
Coordinates 3912672..3912911 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOT98_RS21445 (3907443) 3907443..3907778 + 336 WP_000871291.1 anti-adapter protein IraM -
AOT98_RS29405 (3908059) 3908059..3908167 - 109 Protein_3831 DUF3927 family protein -
AOT98_RS21470 (3909088) 3909088..3909909 - 822 WP_000762882.1 antitermination protein -
AOT98_RS21475 (3909924) 3909924..3910280 - 357 WP_000904111.1 RusA family crossover junction endodeoxyribonuclease -
AOT98_RS21480 (3910293) 3910293..3911342 - 1050 WP_001265249.1 DUF968 domain-containing protein -
AOT98_RS21485 (3911344) 3911344..3911622 - 279 Protein_3835 hypothetical protein -
AOT98_RS21490 (3911689) 3911689..3911940 - 252 WP_000980987.1 protein Rem -
AOT98_RS21495 (3912158) 3912158..3912313 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
AOT98_RS21500 (3912385) 3912385..3912672 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
AOT98_RS21505 (3912672) 3912672..3912911 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
AOT98_RS28260 (3912936) 3912936..3913241 + 306 WP_071818640.1 hypothetical protein -
AOT98_RS21510 (3913238) 3913238..3914394 - 1157 Protein_3841 IS3-like element IS600 family transposase -
AOT98_RS21515 (3914456) 3914456..3915475 + 1020 Protein_3842 MHS family MFS transporter YdfJ -
AOT98_RS21520 (3915564) 3915564..3917024 + 1461 WP_000527804.1 mannitol dehydrogenase family protein -
AOT98_RS21525 (3917060) 3917060..3917263 - 204 WP_000214712.1 putative selenium delivery protein YdfZ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 3901472..3915475 14003


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T57096 WP_000323025.1 NZ_CP012735:c3912672-3912385 [Shigella flexneri 1a]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T57096 NZ_CP012735:c3912672-3912385 [Shigella flexneri 1a]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT57096 WP_000534858.1 NZ_CP012735:c3912911-3912672 [Shigella flexneri 1a]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT57096 NZ_CP012735:c3912911-3912672 [Shigella flexneri 1a]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References