Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1882906..1883432 Replicon chromosome
Accession NZ_CP044158
Organism Shigella flexneri strain AR-0423

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag F4V35_RS09805 Protein ID WP_000323025.1
Coordinates 1882906..1883193 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag F4V35_RS09810 Protein ID WP_000534858.1
Coordinates 1883193..1883432 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F4V35_RS09750 (1877965) 1877965..1878300 + 336 WP_000871291.1 anti-adapter protein IraM -
F4V35_RS09755 (1878581) 1878581..1878689 - 109 Protein_1887 DUF3927 family protein -
F4V35_RS09775 (1879610) 1879610..1880431 - 822 WP_000762882.1 antitermination protein -
F4V35_RS09780 (1880454) 1880454..1880801 - 348 WP_151101433.1 RusA family crossover junction endodeoxyribonuclease -
F4V35_RS09785 (1880814) 1880814..1881863 - 1050 WP_001265249.1 DUF968 domain-containing protein -
F4V35_RS09790 (1881865) 1881865..1882143 - 279 Protein_1891 hypothetical protein -
F4V35_RS09795 (1882210) 1882210..1882461 - 252 WP_000980987.1 protein Rem -
F4V35_RS09800 (1882679) 1882679..1882834 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
F4V35_RS09805 (1882906) 1882906..1883193 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
F4V35_RS09810 (1883193) 1883193..1883432 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
F4V35_RS09815 (1883457) 1883457..1883762 + 306 WP_071818640.1 hypothetical protein -
F4V35_RS09820 (1883759) 1883759..1884915 - 1157 Protein_1897 IS3 family transposase -
F4V35_RS09825 (1884977) 1884977..1885996 + 1020 Protein_1898 MHS family MFS transporter YdfJ -
F4V35_RS09830 (1886085) 1886085..1887545 + 1461 WP_000527804.1 mannitol dehydrogenase family protein -
F4V35_RS09835 (1887581) 1887581..1887784 - 204 WP_000214712.1 putative selenium delivery protein YdfZ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1871994..1885996 14002


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T140405 WP_000323025.1 NZ_CP044158:c1883193-1882906 [Shigella flexneri]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T140405 NZ_CP059155:c2283502-2283398 [Staphylococcus aureus]
TTGTTACTCCTAGAAAGGACTAGCATGTCTGATTTTGAAATGCTGATGATTGTATTAACAATCATTGGTTTAGTATTGAT
TAGTACTCAAGACCATAAAAAATAA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT140405 WP_000534858.1 NZ_CP044158:c1883432-1883193 [Shigella flexneri]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT140405 NZ_CP059155:2283305-2283343 [Staphylococcus aureus]
AACATGTTACCCTAATGAGCCCGTTAAAAAGACGGTGAC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References