Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2547832..2548358 Replicon chromosome
Accession NZ_CP026792
Organism Shigella flexneri strain 61-4982

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag C1P80_RS13375 Protein ID WP_000323025.1
Coordinates 2547832..2548119 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag C1P80_RS13380 Protein ID WP_000534858.1
Coordinates 2548119..2548358 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P80_RS13320 2542890..2543225 + 336 WP_000871291.1 anti-adapter protein IraM -
C1P80_RS13325 2543506..2543614 - 109 Protein_2448 DUF3927 family protein -
C1P80_RS13345 2544535..2545356 - 822 WP_000762884.1 antitermination protein -
C1P80_RS13350 2545371..2545727 - 357 WP_000904111.1 RusA family crossover junction endodeoxyribonuclease -
C1P80_RS13355 2545740..2546789 - 1050 WP_001265249.1 DUF968 domain-containing protein -
C1P80_RS13360 2546791..2547069 - 279 Protein_2452 hypothetical protein -
C1P80_RS13365 2547136..2547387 - 252 WP_000980987.1 hypothetical protein -
C1P80_RS13370 2547605..2547760 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
C1P80_RS13375 2547832..2548119 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
C1P80_RS13380 2548119..2548358 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
C1P80_RS13385 2548383..2548688 + 306 WP_071818640.1 hypothetical protein -
C1P80_RS13390 2548685..2549563 - 879 Protein_2458 IS3 family transposase -
C1P80_RS13395 2549581..2550278 - 698 Protein_2459 IS1 family transposase -
C1P80_RS13400 2550335..2550763 + 429 Protein_2460 fimbrial protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2539693..2555722 16029


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T95962 WP_000323025.1 NZ_CP026792:c2548119-2547832 [Shigella flexneri]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T95962 NZ_CP026792:c2548119-2547832 [Shigella flexneri]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT95962 WP_000534858.1 NZ_CP026792:c2548358-2548119 [Shigella flexneri]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT95962 NZ_CP026792:c2548358-2548119 [Shigella flexneri]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References