Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 750653..751179 Replicon chromosome
Accession NZ_CP026788
Organism Shigella flexneri 2a strain ATCC 29903

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag C1P79_RS04075 Protein ID WP_000323025.1
Coordinates 750892..751179 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag C1P79_RS04070 Protein ID WP_000534858.1
Coordinates 750653..750892 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C1P79_RS04050 (746837) 746837..747856 - 1020 Protein_760 MHS family MFS transporter YdfJ -
C1P79_RS04055 (747918) 747918..749074 + 1157 WP_094105058.1 IS3-like element IS600 family transposase -
C1P79_RS04060 (749071) 749071..749349 - 279 WP_014532248.1 hypothetical protein -
C1P79_RS04065 (749425) 749425..750593 + 1169 WP_000747032.1 IS3-like element IS911 family transposase -
C1P79_RS04070 (750653) 750653..750892 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
C1P79_RS04075 (750892) 750892..751179 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
C1P79_RS04080 (751251) 751251..751406 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
C1P79_RS04085 (751624) 751624..751875 + 252 WP_000980987.1 protein Rem -
C1P79_RS04090 (751942) 751942..752220 + 279 Protein_768 hypothetical protein -
C1P79_RS04095 (752222) 752222..753271 + 1050 WP_001265249.1 DUF968 domain-containing protein -
C1P79_RS04100 (753284) 753284..753640 + 357 WP_000904111.1 RusA family crossover junction endodeoxyribonuclease -
C1P79_RS04105 (753655) 753655..754476 + 822 WP_000762882.1 antitermination protein -
C1P79_RS04125 (755397) 755397..755505 + 109 Protein_772 DUF3927 family protein -
C1P79_RS04130 (755786) 755786..756121 - 336 WP_000871291.1 anti-adapter protein IraM -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 746837..762568 15731


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T95941 WP_000323025.1 NZ_CP026788:750892-751179 [Shigella flexneri 2a]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T95941 NZ_CP026788:750892-751179 [Shigella flexneri 2a]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT95941 WP_000534858.1 NZ_CP026788:750653-750892 [Shigella flexneri 2a]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT95941 NZ_CP026788:750653-750892 [Shigella flexneri 2a]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References