Detailed information    

experimental Experimentally validated

Overview

Name   comX/sigX   Type   Regulator
Locus tag   SMU_RS09085 Genome accession   NC_004350
Coordinates   1872021..1872503 (-) Length   160 a.a.
NCBI ID   WP_002263585.1    Uniprot ID   A0AAX1K1V7
Organism   Streptococcus mutans UA159     
Function   activate transcription of late competence genes   
Competence regulation

Function

The ComS pre-peptide is exported and processed via an unknown mechanism into XIP. XIP is re-imported via oligopeptide permease (Opp) and binds ComR. The ComR/XIP complex binds upstream of comS and sigX at PComR-box sites. The alternative sigma factor, SigX, facilitates RNA polymerase (RNAP) binding at Pcin-box sites. Genes regulated by SigX include the DNA-uptake and transformasome machinery.


Genomic Context

Location: 1867021..1877503
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SMU_RS09065 (SMU.1993) - 1868430..1869242 (-) 813 WP_002263429.1 metal ABC transporter permease -
  SMU_RS09070 (SMU.1994) - 1869232..1869942 (-) 711 WP_002263428.1 metal ABC transporter ATP-binding protein -
  SMU_RS09075 (SMU.1995c) - 1869945..1870391 (-) 447 WP_002263427.1 zinc-dependent MarR family transcriptional regulator -
  SMU_RS09080 (SMU.1996) ispE 1871026..1871874 (-) 849 WP_002352368.1 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase -
  SMU_RS09085 (SMU.1997) comX/sigX 1872021..1872503 (-) 483 WP_002263585.1 sigma-70 family RNA polymerase sigma factor Regulator

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  comX/sigX late competence genes positive effect
  comX/sigX late competence genes positive effect
  scnR comX/sigX positive effect
  hdrR comX/sigX positive effect
  mecA comX/sigX negative effect
  clpC comX/sigX negative effect
  comR comX/sigX positive effect
  clpP comX/sigX negative effect
  XrpA comX/sigX negative effect
  scnK comX/sigX positive effect
  comS comX/sigX positive effect
  comD/blpH comX/sigX positive effect
  vicK comX/sigX negative effect
  comE/blpR comX/sigX positive effect
  scnC comX/sigX positive effect
  brsR comX/sigX positive effect
  cipB comX/sigX positive effect
  vicR comX/sigX negative effect
  comC/blpC comX/sigX positive effect
  hdrR comR positive effect
  comR comS positive effect
  cipB comR positive effect
  XrpA comR negative effect
  hdrM hdrR negative effect
  comS comS positive effect
  oppD comS positive effect
  cipB comS positive effect
  XrpA comS negative effect
  comD/blpH comE/blpR positive effect
  comE/blpR comD/blpH positive effect
  comE/blpR comC/blpC positive effect
  comE/blpR comA/nlmT positive effect
  comE/blpR comE/blpR positive effect
  comE/blpR comB/nlmE positive effect
  vicR comE/blpR negative effect
  vicK comE/blpR negative effect
  vicR comD/blpH negative effect
  vicR comC/blpC negative effect
  vicK comD/blpH negative effect
  vicK comC/blpC negative effect
  comC/blpC comD/blpH positive effect
  comA/nlmT comC/blpC positive effect
  comB/nlmE comC/blpC positive effect
  ciaH comC/blpC positive effect
  sepM comC/blpC positive effect
  brsM brsR negative effect

Sequence

Protein


Download         Length: 160 a.a.        Molecular weight: 19605.49 Da        Isoelectric Point: 6.4997

>NTDB_id=44 SMU_RS09085 WP_002263585.1 1872021..1872503(-) (comX/sigX) [Streptococcus mutans UA159]
MEEDFEIVFNKVKPIVWKLSRYYFIKMWTREDWQQEGMLILHQLLREHPELEEDDTKLYIYFKTRFSNYIKDVLRQQESQ
KRRFNRMSYEEVGEIEHCLSSGGMQLDEYILFRDSLLAYKQGLSTEKQELFERLVAGEHFLGRQSMLKDLRKKLSDFKEK

Nucleotide


Download         Length: 483 bp        

>NTDB_id=44 SMU_RS09085 WP_002263585.1 1872021..1872503(-) (comX/sigX) [Streptococcus mutans UA159]
ATGGAAGAAGATTTTGAAATTGTTTTTAATAAGGTTAAGCCAATTGTATGGAAATTAAGCCGTTATTACTTTATTAAAAT
GTGGACTCGTGAAGATTGGCAACAAGAGGGAATGTTGATTTTGCACCAATTATTAAGGGAACATCCAGAATTAGAAGAGG
ATGATACAAAATTGTATATCTATTTTAAGACACGTTTTTCTAATTACATTAAAGATGTTTTGCGTCAGCAAGAAAGTCAG
AAACGTCGTTTTAATAGAATGTCTTATGAAGAAGTCGGTGAGATTGAACACTGTTTGTCAAGTGGCGGTATGCAATTGGA
TGAATATATTTTATTTCGTGATAGTTTGCTTGCATATAAACAAGGTCTGAGTACTGAAAAGCAAGAGCTGTTTGAGCGCT
TGGTAGCAGGAGAGCACTTTTTGGGAAGGCAAAGTATGCTGAAAGATTTACGTAAAAAATTAAGTGATTTTAAGGAAAAA
TAG

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comR/comR1 Streptococcus gordonii str. Challis substr. CH1

47.436

97.5

0.463
  comR/comR2 Streptococcus gordonii str. Challis substr. CH1

47.436

97.5

0.463
  comX/sigX/comX2/sigX2 Streptococcus mitis NCTC 12261

47.712

96.226

0.459
  comX/sigX/comX1/sigX1 Streptococcus mitis SK321

47.059

96.226

0.453
  comX/sigX Streptococcus infantarius subsp. infantarius ATCC BAA-102

45.513

98.734

0.449
  comX/sigX/comX1/sigX1 Streptococcus mitis NCTC 12261

46.405

96.226

0.447
  comX/sigX/comX2/sigX2 Streptococcus mitis SK321

45.752

96.226

0.44
  comX/comX1 Streptococcus pneumoniae TIGR4

46

94.34

0.434
  comX/comX2 Streptococcus pneumoniae TIGR4

46

94.34

0.434
  comX/comX1 Streptococcus pneumoniae R6

46

94.34

0.434
  comX/comX2 Streptococcus pneumoniae R6

46

94.34

0.434
  comX/comX1 Streptococcus pneumoniae D39

46

94.34

0.434
  comX/comX2 Streptococcus pneumoniae D39

46

94.34

0.434
  comX/comX1 Streptococcus pneumoniae Rx1

46

94.34

0.434
  comX/comX2 Streptococcus pneumoniae Rx1

46

94.34

0.434
  comX/sigX/comX2/sigX2 Streptococcus pyogenes MGAS315

45.098

95.625

0.431
  comX/sigX/comX1/sigX1 Streptococcus pyogenes MGAS315

45.098

95.625

0.431
  comX/sigX Streptococcus suis P1/7

43.226

99.359

0.429
  comX/sigX Streptococcus suis D9

43.226

99.359

0.429
  comX/sigX Streptococcus suis isolate S10

43.226

99.359

0.429
  comX/sigX/comX2/sigX2 Streptococcus pyogenes JRS4

44.444

95.625

0.425
  comX/sigX/comX1/sigX1 Streptococcus pyogenes JRS4

44.444

95.625

0.425
  comX/sigX/comX1/sigX1 Streptococcus pyogenes MGAS8232

44.444

95.625

0.425
  comX/sigX/comX2/sigX2 Streptococcus pyogenes MGAS8232

44.444

95.625

0.425
  comX Streptococcus sobrinus strain NIDR 6715-7

42.581

96.875

0.413
  comX/sigX Streptococcus salivarius strain HSISS4

41.667

97.5

0.406
  comX Streptococcus salivarius SK126

41.667

97.5

0.406
  comX Streptococcus thermophilus LMD-9

41.026

97.5

0.4
  comX Streptococcus thermophilus LMG 18311

41.026

97.5

0.4

Multiple sequence alignment    



References

[1] Antonio Pedro Ricomini Filho et al. (2019) Conserved Pheromone Production, Response and Degradation by Streptococcus mutans. Frontiers in Microbiology 10:2140. [PMID: 31572344]
[2] Simon A M Underhill et al. (2018) Intracellular Signaling by the comRS System in Streptococcus mutans Genetic Competence. MSphere 3(5):e00444-18. [PMID: 30381353]
[3] Iwona B Wenderska et al. (2017) Transcriptional Profiling of the Oral Pathogen Streptococcus mutans in Response to Competence Signaling Peptide XIP. MSystems 2(1):e00102-16. [PMID: 28066817]
[4] Justin Kaspar et al. (2017) Intercellular Communication via the comX-Inducing Peptide (XIP) of Streptococcus mutans. Journal of Bacteriology 199(21):e00404-17. [PMID: 28808131]
[5] R Khan et al. (2016) Comprehensive Transcriptome Profiles of Streptococcus mutans UA159 Map Core Streptococcal Competence Genes. MSystems 1(2):e00038-15. [PMID: 27822519]
[6] Michael Reck et al. (2015) The Alternative Sigma Factor SigX Controls Bacteriocin Synthesis and Competence, the Two Quorum Sensing Regulated Traits in Streptococcus mutans. PLoS Genetics 11(7):e1005353. [PMID: 26158727]
[7] Minjun Son et al. (2015) Bidirectional signaling in the competence regulatory pathway of Streptococcus mutans. FEMS Microbiology Letters 362(19):fnv159. [PMID: 26363019]
[8] Gaofeng Dong et al. (2014) Regulated proteolysis of the alternative sigma factor SigX in Streptococcus mutans: implication in the escape from competence. BMC Microbiology 14:183. [PMID: 25005884]
[9] Kunal Desai et al. (2012) Development of competence for genetic transformation of Streptococcus mutans in a chemically defined medium. Journal of Bacteriology 194(15):3774-80. [PMID: 22609913]
[10] Minjun Son et al. (2012) Microfluidic study of competence regulation in Streptococcus mutans: environmental inputs modulate bimodal and unimodal expression of comX. Molecular Microbiology 86(2):258-72. [PMID: 22845615]
[11] Iwona B Wenderska et al. (2012) A novel function for the competence inducing peptide, XIP, as a cell death effector of Streptococcus mutans. FEMS Microbiology Letters 336(2):104-12. [PMID: 22900705]
[12] Lauren Mashburn-Warren et al. (2010) A novel double-tryptophan peptide pheromone controls competence in Streptococcus spp. via an Rgg regulator. Molecular Microbiology 78(3):589-606. [PMID: 20969646]
[13] Julie A Perry et al. (2009) Peptide alarmone signalling triggers an auto-active bacteriocin necessary for genetic competence. Molecular Microbiology 72(4):905-17. [PMID: 19400789]