Detailed information    

experimental Experimentally validated

Overview

Name   comB/nlmE   Type   Regulator
Locus tag   SMU_RS01430 Genome accession   NC_004350
Coordinates   272924..273826 (+) Length   300 a.a.
NCBI ID   WP_002263524.1    Uniprot ID   A0AAX1K0S8
Organism   Streptococcus mutans UA159     
Function   transport of ComC   
Competence regulation

Function

CSP and the mutacins are exported outside the cell by the same ABC transporter, NlmTE.


Genomic Context

Location: 267924..278826
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SMU_RS01405 (SMU.281) - 267970..268248 (+) 279 WP_002264456.1 hypothetical protein -
  SMU_RS01410 - 268857..269042 (+) 186 WP_002309971.1 hypothetical protein -
  SMU_RS01415 (SMU.283) - 269323..269541 (+) 219 WP_002278706.1 Blp family class II bacteriocin -
  SMU_RS01420 (SMU.284) - 269566..269973 (+) 408 WP_002263726.1 hypothetical protein -
  SMU_RS01425 (SMU.286) comA/nlmT 270628..272910 (+) 2283 WP_002263523.1 peptide cleavage/export ABC transporter Regulator
  SMU_RS01430 (SMU.287) comB/nlmE 272924..273826 (+) 903 WP_002263524.1 HlyD family efflux transporter periplasmic adaptor subunit Regulator
  SMU_RS01435 (SMU.289) - 274183..275850 (+) 1668 WP_002263525.1 BglG family transcription antiterminator -
  SMU_RS01440 (SMU.290) ulaG 276429..277520 (+) 1092 WP_002263526.1 L-ascorbate 6-phosphate lactonase -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  comB/nlmE comC/blpC positive effect
  comC/blpC comX/sigX positive effect
  comX/sigX late competence genes positive effect
  comX/sigX late competence genes positive effect
  scnR comX/sigX positive effect
  hdrR comX/sigX positive effect
  mecA comX/sigX negative effect
  clpC comX/sigX negative effect
  comR comX/sigX positive effect
  clpP comX/sigX negative effect
  XrpA comX/sigX negative effect
  scnK comX/sigX positive effect
  comS comX/sigX positive effect
  comD/blpH comX/sigX positive effect
  vicK comX/sigX negative effect
  comE/blpR comX/sigX positive effect
  scnC comX/sigX positive effect
  brsR comX/sigX positive effect
  cipB comX/sigX positive effect
  vicR comX/sigX negative effect
  hdrR comR positive effect
  comR comS positive effect
  cipB comR positive effect
  XrpA comR negative effect
  hdrM hdrR negative effect
  comS comS positive effect
  oppD comS positive effect
  cipB comS positive effect
  XrpA comS negative effect
  comD/blpH comE/blpR positive effect
  comE/blpR comD/blpH positive effect
  comE/blpR comC/blpC positive effect
  comE/blpR comA/nlmT positive effect
  comE/blpR comE/blpR positive effect
  comE/blpR comB/nlmE positive effect
  vicR comE/blpR negative effect
  vicK comE/blpR negative effect
  vicR comD/blpH negative effect
  vicR comC/blpC negative effect
  vicK comD/blpH negative effect
  vicK comC/blpC negative effect
  comC/blpC comD/blpH positive effect
  comA/nlmT comC/blpC positive effect
  ciaH comC/blpC positive effect
  sepM comC/blpC positive effect
  brsM brsR negative effect

Sequence

Protein


Download         Length: 300 a.a.        Molecular weight: 34116.49 Da        Isoelectric Point: 10.4213

>NTDB_id=359 SMU_RS01430 WP_002263524.1 272924..273826(+) (comB/nlmE) [Streptococcus mutans UA159]
MDPKFLQSAEFYRRRYHNFATLLIVPLVCLIIFLVIFLCFAKKEITVISTGEVAPTKVVDVIQSYSDSSIIKNNLDNNAA
VEKGDVLIEYSENASPNRQTEQKNIIKERQKREEKEKKKHQKSKKKKKSKSKKASKDKKKKSKDKESSSDDENETKKVSI
FASEDGIIHTNPKYDGANIIPKQTEIAQIYPDIQKTRKVLITYYASSDDVVSMKKGQTARLSLEKKGNDKVVIEGKINNV
ASSATTTKKGNLFKVTAKVKVSKKNSKLIKYGMTGKTVTVIDKKTYFDYFKDKLLHKMDN

Nucleotide


Download         Length: 903 bp        

>NTDB_id=359 SMU_RS01430 WP_002263524.1 272924..273826(+) (comB/nlmE) [Streptococcus mutans UA159]
ATGGATCCTAAATTTTTACAAAGTGCAGAATTTTATAGGAGACGCTATCATAATTTTGCGACACTATTAATTGTTCCTTT
GGTCTGCTTGATTATCTTCTTGGTCATATTCCTTTGTTTTGCTAAAAAAGAAATTACAGTGATTTCTACTGGTGAAGTTG
CACCAACAAAGGTTGTAGATGTTATCCAATCTTACAGTGACAGTTCAATCATTAAAAATAATTTAGATAATAATGCAGCT
GTTGAGAAGGGAGACGTTTTAATTGAATATTCAGAAAATGCCAGTCCAAACCGTCAGACTGAACAAAAGAATATTATAAA
AGAAAGACAAAAACGAGAAGAGAAGGAAAAGAAAAAACACCAAAAGAGCAAGAAAAAGAAGAAGTCTAAGAGCAAGAAAG
CTTCCAAAGATAAGAAAAAGAAATCGAAAGACAAGGAAAGCAGCTCTGACGATGAAAATGAGACAAAAAAGGTTTCGATT
TTTGCTTCAGAAGATGGTATTATTCATACCAATCCCAAATATGATGGTGCCAATATTATTCCGAAGCAAACCGAGATTGC
TCAAATCTATCCTGATATTCAAAAAACAAGAAAAGTGTTAATCACCTATTATGCTTCTTCTGATGATGTTGTTTCTATGA
AAAAGGGGCAAACCGCTCGTCTTTCCTTGGAAAAAAAGGGAAATGACAAGGTTGTTATTGAAGGAAAAATTAACAATGTC
GCTTCATCAGCAACTACTACTAAAAAAGGAAATCTCTTTAAGGTTACTGCCAAAGTAAAGGTTTCTAAGAAAAATAGCAA
ACTCATCAAGTATGGTATGACAGGCAAGACAGTCACTGTCATTGATAAAAAGACTTATTTTGATTATTTCAAAGATAAAT
TACTGCATAAAATGGATAATTAG

Domains

Predicted by InterproScan.

HlyD family secretion protein (HlyD_3)

(160-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comB Streptococcus mutans UA159

50.299

99.11

0.499

Multiple sequence alignment    



References

[1] Mohammad Shahnoor Hossain et al. (2012) An extracelluar protease, SepM, generates functional competence-stimulating peptide in Streptococcus mutans UA159. Journal of Bacteriology 194(21):5886-96. [PMID: 22923597]
[2] John D F Hale et al. (2005) Identification of nlmTE, the locus encoding the ABC transport system required for export of nonlantibiotic mutacins in Streptococcus mutans. Journal of Bacteriology 187(14):5036-9. [PMID: 15995224]