Detailed information    

experimental Experimentally validated

Overview

Name   scnR   Type   Regulator
Locus tag   SMU_RS05280 Genome accession   NC_004350
Coordinates   1086160..1086849 (-) Length   229 a.a.
NCBI ID   WP_002262198.1    Uniprot ID   Q8DU13
Organism   Streptococcus mutans UA159     
Function   regulate comX expression   
Competence regulation

Function

Deletion of the small scnC gene, or the whole scnC/R/K operon leads to a ~10-fold reduction in comX expression and natural transformation frequencies.


Genomic Context

Location: 1081160..1091849
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SMU_RS05250 (SMU.1140c) - 1081411..1082169 (-) 759 WP_002262204.1 inositol monophosphatase family protein -
  SMU_RS05255 (SMU.1141c) - 1082159..1082437 (-) 279 WP_002279684.1 UPF0223 family protein -
  SMU_RS05260 (SMU.1142c) spx 1082430..1082843 (-) 414 WP_002263835.1 transcriptional regulator Spx -
  SMU_RS05265 (SMU.1143c) - 1082904..1083824 (-) 921 WP_002262201.1 bifunctional riboflavin kinase/FAD synthetase -
  SMU_RS05270 (SMU.1144) truB 1083855..1084736 (-) 882 WP_002262200.1 tRNA pseudouridine(55) synthase TruB -
  SMU_RS05275 (SMU.1145c) scnK 1084846..1086159 (-) 1314 WP_002262199.1 sensor histidine kinase Regulator
  SMU_RS05280 (SMU.1146c) scnR 1086160..1086849 (-) 690 WP_002262198.1 response regulator transcription factor Regulator
  - scnC 1086868..1087053 (-) 186 - - Regulator
  SMU_RS05285 (SMU.1148) - 1087024..1087932 (+) 909 WP_002262197.1 ABC transporter ATP-binding protein -
  SMU_RS05290 (SMU.1149) - 1087932..1088672 (+) 741 WP_002262196.1 ABC transporter permease -
  SMU_RS05295 (SMU.1150) - 1088669..1089397 (+) 729 WP_002262195.1 ABC transporter permease -
  SMU_RS05300 (SMU.1151c) - 1089516..1090880 (-) 1365 WP_002262194.1 DUF438 domain-containing protein -
  SMU_RS05305 (SMU.1152c) - 1090880..1091113 (-) 234 WP_002262193.1 DUF1858 domain-containing protein -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  scnR comX/sigX positive effect
  comX/sigX late competence genes positive effect
  comX/sigX late competence genes positive effect
  hdrR comX/sigX positive effect
  hdrR comR positive effect
  hdrM hdrR negative effect
  mecA comX/sigX negative effect
  clpC comX/sigX negative effect
  comR comX/sigX positive effect
  comR comS positive effect
  cipB comR positive effect
  XrpA comR negative effect
  clpP comX/sigX negative effect
  XrpA comX/sigX negative effect
  XrpA comS negative effect
  scnK comX/sigX positive effect
  comS comX/sigX positive effect
  comS comS positive effect
  oppD comS positive effect
  cipB comS positive effect
  comD/blpH comX/sigX positive effect
  comD/blpH comE/blpR positive effect
  vicR comD/blpH negative effect
  comE/blpR comD/blpH positive effect
  vicK comD/blpH negative effect
  comC/blpC comD/blpH positive effect
  vicK comX/sigX negative effect
  vicK comC/blpC negative effect
  vicK comE/blpR negative effect
  comE/blpR comX/sigX positive effect
  comE/blpR comC/blpC positive effect
  comE/blpR comA/nlmT positive effect
  comE/blpR comE/blpR positive effect
  comE/blpR comB/nlmE positive effect
  vicR comE/blpR negative effect
  scnC comX/sigX positive effect
  brsR comX/sigX positive effect
  brsM brsR negative effect
  cipB comX/sigX positive effect
  vicR comX/sigX negative effect
  vicR comC/blpC negative effect
  comC/blpC comX/sigX positive effect
  comA/nlmT comC/blpC positive effect
  comB/nlmE comC/blpC positive effect
  ciaH comC/blpC positive effect
  sepM comC/blpC positive effect

Sequence

Protein


Download         Length: 229 a.a.        Molecular weight: 26243.58 Da        Isoelectric Point: 6.9650

>NTDB_id=372 SMU_RS05280 WP_002262198.1 1086160..1086849(-) (scnR) [Streptococcus mutans UA159]
MNYKEKKILILDDNPEILEMVQESLNIAGFNNLTGVQSKKEALEQLGKRSFDLAILDIMLPDGSGFEVLREIRKTSTIPV
LFLSAVSDIEKQYQGFELGADDYIVKPFRPKELELRILSILKRAYPEKDDTLILPTCQVNFSQALITKGELEIQLTAKEY
SILKVLYNNQNRIVTFDQLLEKVWGLQYQGYENTMMAHIRKIRQKIEANPSKPECLLTVKGLGYKLKVS

Nucleotide


Download         Length: 690 bp        

>NTDB_id=372 SMU_RS05280 WP_002262198.1 1086160..1086849(-) (scnR) [Streptococcus mutans UA159]
ATGAATTATAAAGAGAAAAAAATCTTAATTTTAGATGACAATCCAGAAATTTTAGAAATGGTGCAAGAGTCTTTAAACAT
TGCTGGTTTTAACAACCTAACAGGCGTGCAATCAAAAAAAGAGGCCTTGGAGCAACTTGGCAAAAGATCCTTTGATTTGG
CCATTTTAGACATTATGCTGCCTGATGGTTCGGGTTTTGAGGTACTAAGAGAAATTCGGAAAACTTCTACTATACCAGTA
CTCTTTTTGTCTGCGGTATCCGACATTGAAAAGCAATACCAAGGTTTTGAGTTGGGAGCGGACGACTACATCGTCAAACC
TTTTCGTCCTAAGGAATTGGAGTTGCGAATCCTTTCTATTTTAAAAAGAGCCTATCCTGAAAAGGACGATACCCTTATTT
TACCTACTTGTCAGGTCAACTTTAGTCAAGCTTTGATTACCAAAGGAGAATTGGAAATTCAGCTGACAGCTAAGGAATAC
AGTATTCTTAAAGTTTTATATAACAATCAAAATCGCATTGTTACCTTTGATCAACTCTTAGAAAAAGTTTGGGGTCTGCA
ATATCAAGGCTATGAAAATACTATGATGGCGCATATTCGCAAGATCAGGCAAAAGATTGAAGCCAATCCTTCTAAGCCAG
AATGTTTACTGACAGTTAAGGGCTTGGGTTACAAACTAAAGGTGAGTTAA

Domains

Predicted by InterproScan.

Response regulator receiver domain (Response_reg)

(8-117)

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(152-226)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8DU13

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  micA Streptococcus pneumoniae Cp1015

38.197

100

0.389
  vicR Streptococcus mutans UA159

36.207

100

0.367

Multiple sequence alignment    



References

[1] Jeong Nam Kim et al. (2013) Core-gene-encoded peptide regulating virulence-associated traits in Streptococcus mutans. Journal of Bacteriology 195(12):2912-20. [PMID: 23603743]