Detailed information    

experimental Experimentally validated

Overview

Name   ciaH   Type   Regulator
Locus tag   SMU_RS05195 Genome accession   NC_004350
Coordinates   1069642..1070949 (-) Length   435 a.a.
NCBI ID   WP_002262215.1    Uniprot ID   -
Organism   Streptococcus mutans UA159     
Function   Required for optimal comC expression   
Competence regulation

Function

The histidine kinase CiaH, but not the response regulator CiaR, causes a dramatic decrease in biofilm formation and in transformation efficiency. CiaH was shown to be required for optimal comC expression.


Genomic Context

Location: 1064642..1075949
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SMU_RS05165 (SMU.1122) - 1065189..1065575 (-) 387 WP_002262221.1 cytidine deaminase -
  SMU_RS05170 (SMU.1123) deoC 1065565..1066227 (-) 663 WP_002262220.1 deoxyribose-phosphate aldolase -
  SMU_RS05175 (SMU.1124) - 1066246..1067523 (-) 1278 WP_002262219.1 pyrimidine-nucleoside phosphorylase -
  SMU_RS05180 (SMU.1125c) - 1067632..1068228 (-) 597 WP_002262218.1 class I SAM-dependent methyltransferase -
  SMU_RS05185 (SMU.1126) coaA 1068324..1069244 (+) 921 WP_002262217.1 type I pantothenate kinase -
  SMU_RS05190 (SMU.1127) rpsT 1069298..1069552 (+) 255 WP_011074586.1 30S ribosomal protein S20 -
  SMU_RS05195 (SMU.1128) ciaH 1069642..1070949 (-) 1308 WP_002262215.1 three-component system sensor histidine kinase CiaH Regulator
  SMU_RS05200 (SMU.1129) ciaR 1070939..1071613 (-) 675 WP_002262214.1 three-component system response regulator CiaR Regulator
  SMU_RS05205 (SMU.1131c) ciaX 1071664..1071927 (-) 264 WP_002262213.1 three-component system regulator CiaX -
  SMU_RS05210 (SMU.1132) - 1072328..1074877 (-) 2550 WP_002262212.1 M1 family metallopeptidase -
  SMU_RS05215 (SMU.1133) phoU 1074918..1075571 (-) 654 WP_002262211.1 phosphate signaling complex protein PhoU -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  ciaH comC/blpC positive effect
  comC/blpC comX/sigX positive effect
  comX/sigX late competence genes positive effect
  comX/sigX late competence genes positive effect
  scnR comX/sigX positive effect
  hdrR comX/sigX positive effect
  mecA comX/sigX negative effect
  clpC comX/sigX negative effect
  comR comX/sigX positive effect
  clpP comX/sigX negative effect
  XrpA comX/sigX negative effect
  scnK comX/sigX positive effect
  comS comX/sigX positive effect
  comD/blpH comX/sigX positive effect
  vicK comX/sigX negative effect
  comE/blpR comX/sigX positive effect
  scnC comX/sigX positive effect
  brsR comX/sigX positive effect
  cipB comX/sigX positive effect
  vicR comX/sigX negative effect
  hdrR comR positive effect
  comR comS positive effect
  cipB comR positive effect
  XrpA comR negative effect
  hdrM hdrR negative effect
  comS comS positive effect
  oppD comS positive effect
  cipB comS positive effect
  XrpA comS negative effect
  comD/blpH comE/blpR positive effect
  comE/blpR comD/blpH positive effect
  comE/blpR comC/blpC positive effect
  comE/blpR comA/nlmT positive effect
  comE/blpR comE/blpR positive effect
  comE/blpR comB/nlmE positive effect
  vicR comE/blpR negative effect
  vicK comE/blpR negative effect
  vicR comD/blpH negative effect
  vicR comC/blpC negative effect
  vicK comD/blpH negative effect
  vicK comC/blpC negative effect
  comC/blpC comD/blpH positive effect
  comA/nlmT comC/blpC positive effect
  comB/nlmE comC/blpC positive effect
  sepM comC/blpC positive effect
  brsM brsR negative effect

Sequence

Protein


Download         Length: 435 a.a.        Molecular weight: 49868.35 Da        Isoelectric Point: 9.7262

>NTDB_id=363 SMU_RS05195 WP_002262215.1 1069642..1070949(-) (ciaH) [Streptococcus mutans UA159]
MLIKLRKQKQDENISFFSHFFAVFTGIFFVMTVIIIQVMHFGIYSSVDNSLKIATENVNDYVNMTLSHNNPITEAENGDA
SFKVKSNGQMAANTDTILYDKKHNVINNVNAFSSLYHIRPKLKDIGDIVEQEAKNIYGQTEKYRTVTVKVNNDFYPQIKF
ATIVINTTQLEEANTRYVTIIISVMIVFWLISVIASVYLAKWSQKPILENYEKQKSFVENASHELRTPLAVLQNRLESLF
RKPEATILDSSESIASSLEEVRNMRMLTANLLNLARRDDGIKPEITEIQPRFFDDTFKNYEMIARENGKKLHIINKVNRQ
IKTDKTLLKQLMTILFDNAVKYTDNDGEIWLDVKTTDRSLIFSVADNGQGISDEDKKKIFDRFYRVDKARTRQKGGFGLG
LSLAKQITDSLKGSIIVKDKKPQGVMFEARLLANK

Nucleotide


Download         Length: 1308 bp        

>NTDB_id=363 SMU_RS05195 WP_002262215.1 1069642..1070949(-) (ciaH) [Streptococcus mutans UA159]
ATGCTAATTAAATTAAGAAAACAAAAGCAGGATGAGAATATTTCATTTTTCAGTCATTTCTTTGCTGTTTTTACCGGAAT
ATTCTTTGTTATGACAGTTATTATTATTCAGGTTATGCATTTTGGTATCTATTCTTCAGTTGATAATAGTCTAAAAATAG
CAACTGAGAATGTCAATGATTATGTCAATATGACTCTATCGCATAATAATCCTATTACTGAAGCTGAAAATGGAGATGCC
TCTTTTAAAGTAAAATCAAATGGCCAGATGGCTGCTAATACAGATACTATTCTTTATGATAAAAAGCATAACGTCATCAA
TAATGTCAATGCCTTCTCTTCTTTGTATCATATTCGCCCTAAACTAAAAGATATTGGTGATATTGTTGAACAAGAAGCTA
AGAATATTTACGGTCAGACCGAAAAGTATCGGACAGTGACAGTTAAGGTAAATAATGATTTTTACCCACAGATAAAATTT
GCAACAATTGTTATTAATACAACACAGCTTGAAGAGGCTAATACGCGTTATGTTACGATTATTATCAGTGTGATGATTGT
TTTTTGGCTTATATCAGTTATTGCCAGTGTTTATTTAGCGAAGTGGAGTCAAAAACCGATTCTGGAAAATTATGAAAAGC
AAAAATCATTTGTTGAAAATGCCAGTCATGAATTACGTACTCCTTTGGCTGTTTTGCAAAATCGCTTGGAGAGTTTGTTT
CGAAAACCAGAGGCTACTATCTTAGATTCTTCGGAATCTATTGCTTCTAGTTTGGAAGAGGTCAGAAACATGAGAATGCT
GACAGCAAATCTCCTTAATTTAGCCCGTCGGGATGATGGTATCAAACCAGAAATAACAGAAATTCAACCTCGTTTTTTTG
ATGATACTTTTAAAAATTATGAGATGATCGCTCGAGAAAATGGGAAAAAATTGCATATTATCAATAAGGTCAACCGTCAA
ATTAAGACAGATAAGACTTTGCTGAAGCAATTAATGACTATTCTTTTTGATAATGCTGTCAAATATACAGATAACGACGG
GGAAATTTGGCTGGACGTCAAGACAACTGATCGCAGTTTAATTTTTTCAGTTGCGGACAATGGTCAAGGTATTTCAGACG
AGGATAAGAAGAAAATTTTTGATCGTTTTTATCGCGTCGATAAAGCCAGAACACGACAAAAGGGTGGGTTCGGACTTGGT
TTGTCTTTGGCAAAACAAATAACAGACAGCTTAAAAGGCAGCATTATTGTTAAAGATAAAAAACCACAAGGAGTAATGTT
TGAAGCTCGCCTGCTTGCTAATAAATAA

Domains

Predicted by InterproScan.

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(324-431)

His Kinase A (phospho-acceptor) domain (HisKA)

(215-280)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaH Streptococcus pneumoniae D39

55.632

100

0.556
  ciaH Streptococcus pneumoniae Rx1

55.632

100

0.556
  ciaH Streptococcus pneumoniae TIGR4

55.632

100

0.556
  ciaH Streptococcus pneumoniae R6

55.632

100

0.556

Multiple sequence alignment    



References

[1] Chenggang Wu et al. (2010) Regulation of ciaXRH operon expression and identification of the CiaR regulon in Streptococcus mutans. Journal of Bacteriology 192(18):4669-79. [PMID: 20639331]
[2] Xuesong He et al. (2008) The cia operon of Streptococcus mutans encodes a unique component required for calcium-mediated autoregulation. Molecular Microbiology 70(1):112-26. [PMID: 18681938]
[3] Sang-Joon Ahn et al. (2006) Multilevel control of competence development and stress tolerance in Streptococcus mutans UA159. Infection And Immunity 74(3):1631-42. [PMID: 16495534]