Detailed information    

experimental Experimentally validated

Overview

Name   sepM   Type   Regulator
Locus tag   SMU_RS02495 Genome accession   NC_004350
Coordinates   485643..486683 (+) Length   346 a.a.
NCBI ID   WP_002262039.1    Uniprot ID   -
Organism   Streptococcus mutans UA159     
Function   processing of CSP   
Competence regulation

Function

SepM is a cell surface protease that is involved in the processing of 21-residue CSP to generate the 18-residue CSP. CSP cannot stimulate the competence in the ΔsepM mutant.


Genomic Context

Location: 480643..491683
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SMU_RS02465 - 480892..481095 (+) 204 WP_002263011.1 hypothetical protein -
  SMU_RS02470 (SMU.513) - 481136..481369 (+) 234 WP_002263010.1 hypothetical protein -
  SMU_RS02475 (SMU.514) - 481590..482156 (+) 567 WP_002279620.1 TetR/AcrR family transcriptional regulator -
  SMU_RS02480 (SMU.515) - 482277..484052 (+) 1776 WP_002262036.1 oleate hydratase -
  SMU_RS02485 (SMU.516) rsmD 484636..485175 (+) 540 WP_002262037.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  SMU_RS02490 (SMU.517) coaD 485165..485665 (+) 501 WP_002262038.1 pantetheine-phosphate adenylyltransferase -
  SMU_RS02495 (SMU.518) sepM 485643..486683 (+) 1041 WP_002262039.1 S16 family serine protease SepM Regulator
  SMU_RS02500 (SMU.520) - 486756..488087 (+) 1332 WP_002262040.1 bifunctional metallophosphatase/5'-nucleotidase -
  SMU_RS02505 (SMU.521) - 488084..488632 (+) 549 WP_002262041.1 YutD family protein -
  SMU_RS02510 (SMU.522) rlmN 488629..489714 (+) 1086 WP_002262042.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  SMU_RS02515 (SMU.523) - 489714..490250 (+) 537 WP_002262043.1 VanZ family protein -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  sepM comC/blpC positive effect
  comC/blpC comX/sigX positive effect
  comX/sigX late competence genes positive effect
  comX/sigX late competence genes positive effect
  scnR comX/sigX positive effect
  hdrR comX/sigX positive effect
  mecA comX/sigX negative effect
  clpC comX/sigX negative effect
  comR comX/sigX positive effect
  clpP comX/sigX negative effect
  XrpA comX/sigX negative effect
  scnK comX/sigX positive effect
  comS comX/sigX positive effect
  comD/blpH comX/sigX positive effect
  vicK comX/sigX negative effect
  comE/blpR comX/sigX positive effect
  scnC comX/sigX positive effect
  brsR comX/sigX positive effect
  cipB comX/sigX positive effect
  vicR comX/sigX negative effect
  hdrR comR positive effect
  comR comS positive effect
  cipB comR positive effect
  XrpA comR negative effect
  hdrM hdrR negative effect
  comS comS positive effect
  oppD comS positive effect
  cipB comS positive effect
  XrpA comS negative effect
  comD/blpH comE/blpR positive effect
  comE/blpR comD/blpH positive effect
  comE/blpR comC/blpC positive effect
  comE/blpR comA/nlmT positive effect
  comE/blpR comE/blpR positive effect
  comE/blpR comB/nlmE positive effect
  vicR comE/blpR negative effect
  vicK comE/blpR negative effect
  vicR comD/blpH negative effect
  vicR comC/blpC negative effect
  vicK comD/blpH negative effect
  vicK comC/blpC negative effect
  comC/blpC comD/blpH positive effect
  comA/nlmT comC/blpC positive effect
  comB/nlmE comC/blpC positive effect
  ciaH comC/blpC positive effect
  brsM brsR negative effect

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 37980.92 Da        Isoelectric Point: 10.1735

>NTDB_id=360 SMU_RS02495 WP_002262039.1 485643..486683(+) (sepM) [Streptococcus mutans UA159]
MKTNKKFKWWLISGISLILLLMVFFFPLPYYLEMPGGAYDIRSVVTVNHKEDKEKGSYNFVAVSLSKATPIQVLYAWLTP
FTEITSAKETTGGVSDADYMRINQFYMETSQNGAIYKALSLAHKKASFNYMGVYVLQVSRNSTFKGVLNIADTVTGVNDK
TFHSSKELIKYVSGLKLGSKVSVQYTSENKKKTAKGKVIKLSNGKNGIGIGLVDHTKVTSDDKIEFSTNGIGGPSAGLMF
TLDIYDQLVKEDLRKGRIIAGTGTIGEDGSVGDIGGADMKVAAADRINADIFFVPNNPVSKATLKKNPKALTNYQEAKQA
AKKLGTKMKIVPVKNVQEAIDYLRKH

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=360 SMU_RS02495 WP_002262039.1 485643..486683(+) (sepM) [Streptococcus mutans UA159]
GTGAAAACAAACAAAAAATTTAAATGGTGGCTAATCAGCGGTATCAGTCTTATTTTGCTTTTAATGGTATTCTTTTTTCC
TCTTCCTTACTATTTAGAAATGCCCGGCGGAGCCTATGATATTCGTTCTGTTGTGACGGTTAATCATAAAGAAGATAAAG
AGAAAGGATCTTATAATTTTGTAGCTGTCTCTCTTAGTAAGGCAACCCCCATACAAGTCCTCTATGCTTGGTTGACCCCT
TTTACAGAAATTACCAGTGCCAAGGAAACAACTGGTGGTGTTAGTGATGCGGATTATATGAGGATTAATCAGTTTTATAT
GGAAACCTCACAAAATGGAGCTATCTATAAAGCTCTTTCTTTAGCTCATAAAAAGGCAAGCTTTAATTATATGGGTGTTT
ATGTTTTGCAGGTTAGTAGGAATTCAACATTCAAGGGTGTTTTAAATATTGCTGATACTGTAACAGGCGTTAATGACAAG
ACCTTTCATAGTTCAAAAGAATTGATTAAATATGTGTCTGGACTGAAATTAGGCAGCAAGGTCAGTGTTCAATATACTTC
TGAAAATAAGAAGAAAACAGCCAAGGGCAAAGTTATTAAATTGTCAAATGGCAAAAATGGTATTGGCATTGGCTTGGTTG
ATCATACCAAGGTCACTTCTGATGATAAAATCGAATTTAGCACAAATGGTATTGGAGGGCCAAGTGCCGGTCTCATGTTT
ACCCTTGATATTTACGATCAACTAGTGAAAGAGGACTTACGTAAGGGTCGTATTATTGCAGGTACTGGTACTATTGGAGA
AGACGGGTCTGTTGGGGATATTGGCGGTGCCGATATGAAAGTGGCAGCAGCAGATAGGATTAATGCAGATATCTTTTTTG
TTCCCAATAATCCTGTAAGTAAGGCAACTTTGAAAAAAAATCCCAAAGCTCTCACTAATTATCAAGAAGCTAAGCAGGCT
GCTAAAAAATTAGGCACTAAGATGAAGATTGTCCCTGTTAAAAATGTGCAGGAGGCTATTGATTACCTAAGAAAACACTA
A

Domains

Predicted by InterproScan.

PDZ domain (PDZ_2)

(131-195)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Mohammad Shahnoor Hossain et al. (2012) An extracelluar protease, SepM, generates functional competence-stimulating peptide in Streptococcus mutans UA159. Journal of Bacteriology 194(21):5886-96. [PMID: 22923597]