Detailed information NTG0000380

Experimentally validated

Overview

• Name: mecA
• Type: Regulator
• Locus tag: SMU_RS01250
• Genome accession: NC_004350
• Coordinates: 233844..234566 (+)
• Length: 240 a.a.
• NCBI ID: WP_002262749.1
• Uniprot ID: Q8DW33
• Organism: Streptococcus mutans UA159
• Function: degradation of ComX Competence regulation

Function

MecA interacts with both SigX and ClpC, forming a ternary SigX-MecA-ClpC complex. MecA mediates the formation of a ternary SigX-MecA-ClpC complex that sequesters SigX and thereby negatively regulates genetic competence in S. mutans.

Genomic Context

Location: 228844..239566

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SMU_RS01235 (SMU.242c)	-	229245..230798 (-)	1554	WP_002262752.1	ABC transporter substrate-binding protein/permease	-
SMU_RS01240 (SMU.243)	-	230950..232851 (+)	1902	WP_002262751.1	DUF2207 domain-containing protein	-
SMU_RS01245 (SMU.244)	-	232909..233754 (+)	846	WP_002262750.1	undecaprenyl-diphosphate phosphatase	-
SMU_RS01250 (SMU.245)	mecA	233844..234566 (+)	723	WP_002262749.1	adaptor protein MecA	Regulator
SMU_RS01255 (SMU.246)	rgpG	234572..235732 (+)	1161	WP_002262748.1	rhamnose-glucose polysaccharide biosynthesis protein RgpB	-
SMU_RS01260 (SMU.247)	sufC	235806..236576 (+)	771	WP_002262747.1	Fe-S cluster assembly ATPase SufC	-
SMU_RS01265 (SMU.248)	sufD	236614..237876 (+)	1263	WP_002262746.1	Fe-S cluster assembly protein SufD	-
SMU_RS01270 (SMU.249)	-	237881..239110 (+)	1230	WP_002262745.1	cysteine desulfurase	-
SMU_RS01275 (SMU.250)	sufU	239097..239531 (+)	435	WP_002262744.1	Fe-S cluster assembly sulfur transfer protein SufU	-

Regulatory network

Positive effect      

Negative effect

Regulator	Target	Regulation
mecA	comX/sigX	negative effect
comX/sigX	late competence genes	positive effect
comX/sigX	late competence genes	positive effect
scnR	comX/sigX	positive effect
hdrR	comX/sigX	positive effect
hdrR	comR	positive effect
hdrM	hdrR	negative effect
clpC	comX/sigX	negative effect
comR	comX/sigX	positive effect
comR	comS	positive effect
cipB	comR	positive effect
XrpA	comR	negative effect
clpP	comX/sigX	negative effect
XrpA	comX/sigX	negative effect
XrpA	comS	negative effect
scnK	comX/sigX	positive effect
comS	comX/sigX	positive effect
comS	comS	positive effect
oppD	comS	positive effect
cipB	comS	positive effect
comD/blpH	comX/sigX	positive effect
comD/blpH	comE/blpR	positive effect
vicR	comD/blpH	negative effect
comE/blpR	comD/blpH	positive effect
vicK	comD/blpH	negative effect
comC/blpC	comD/blpH	positive effect
vicK	comX/sigX	negative effect
vicK	comC/blpC	negative effect
vicK	comE/blpR	negative effect
comE/blpR	comX/sigX	positive effect
comE/blpR	comC/blpC	positive effect
comE/blpR	comA/nlmT	positive effect
comE/blpR	comE/blpR	positive effect
comE/blpR	comB/nlmE	positive effect
vicR	comE/blpR	negative effect
scnC	comX/sigX	positive effect
brsR	comX/sigX	positive effect
brsM	brsR	negative effect
cipB	comX/sigX	positive effect
vicR	comX/sigX	negative effect
vicR	comC/blpC	negative effect
comC/blpC	comX/sigX	positive effect
comA/nlmT	comC/blpC	positive effect
comB/nlmE	comC/blpC	positive effect
ciaH	comC/blpC	positive effect
sepM	comC/blpC	positive effect

Sequence

Protein

• Length: 240 a.a.
• Molecular weight: 28055.65 Da
• Isoelectric Point: 4.2340

>NTDB_id=380 SMU_RS01250 WP_002262749.1 233844..234566(+) (mecA) [Streptococcus mutans UA159]
MEMKQISETTLKITISMEDLEERGMELKDFLIPQEKTEEFFYTVMDELDLPENFKDSGMLSFRVTPRNDRIDVFVTKSEI
NKNLNLEDLSDFDDISKMSPEDFFNTLEETMREKGDAAALDKLAEIEKREEEKTQQEKGETKEKRDYVHFVLDFPNIQQV
INFAKTVDYDVEASELFKESDAYHMTVLLNLEDKPDYYADLMFARMLEHAGRGTKTRAYLLEHGVQLIKADALQELQMIG

Nucleotide

• Length: 723 bp

>NTDB_id=380 SMU_RS01250 WP_002262749.1 233844..234566(+) (mecA) [Streptococcus mutans UA159]
ATGGAAATGAAACAAATCAGCGAAACAACATTAAAAATCACCATCAGTATGGAAGATTTAGAAGAAAGAGGCATGGAATT
AAAAGATTTCTTGATTCCGCAGGAAAAGACGGAAGAATTTTTCTATACTGTTATGGATGAGCTGGACTTGCCTGAAAATT
TCAAGGACAGTGGGATGTTAAGCTTTCGAGTAACACCAAGGAATGATCGCATTGATGTTTTTGTCACTAAGTCAGAAATC
AATAAAAATCTTAATTTGGAGGATTTGTCTGATTTTGATGATATTTCAAAAATGTCTCCGGAGGATTTCTTTAATACCTT
GGAGGAAACCATGCGTGAAAAAGGGGATGCTGCAGCTCTGGATAAATTGGCTGAAATTGAAAAAAGAGAAGAAGAGAAGA
CTCAGCAGGAAAAAGGTGAAACTAAGGAAAAAAGAGATTACGTTCACTTTGTGCTGGATTTTCCTAATATTCAGCAAGTC
ATAAATTTTGCTAAGACAGTTGACTATGATGTAGAGGCTTCAGAACTTTTTAAGGAGTCTGACGCTTATCACATGACTGT
TCTGCTTAATCTCGAAGATAAACCCGACTATTATGCTGATCTTATGTTTGCAAGAATGTTGGAGCATGCTGGTAGAGGAA
CAAAGACACGTGCTTATCTATTAGAACATGGAGTTCAGCTCATTAAAGCAGATGCTCTGCAAGAATTACAAATGATTGGA
TAA

Domains

Predicted by InterproScan.

• Negative regulator of genetic competence (MecA) (MecA) (1-236)

3D structure

• AlphaFold DB: Q8DW33

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
mecA	Streptococcus thermophilus LMD-9	57.787	100	0.588
mecA	Streptococcus thermophilus LMG 18311	57.377	100	0.583
mecA	Streptococcus pneumoniae D39	48.571	100	0.496
mecA	Streptococcus pneumoniae Rx1	48.571	100	0.496
mecA	Streptococcus pneumoniae R6	48.571	100	0.496
mecA	Streptococcus pneumoniae TIGR4	48.571	100	0.496

References

• [1]: M Son et al. (2018) Threshold regulation and stochasticity from the MecA/ClpCP proteolytic system in Streptococcus mutans competence. Molecular Microbiology 110(6):914-930. [PMID: 29873131]
• [2]: Gaofeng Dong et al. (2014) Regulated proteolysis of the alternative sigma factor SigX in Streptococcus mutans: implication in the escape from competence. BMC Microbiology 14:183. [PMID: 25005884]
• [3]: Xiao-Lin Tian et al. (2013) MecA protein acts as a negative regulator of genetic competence in Streptococcus mutans. Journal of Bacteriology 195(22):5196-206. [PMID: 24039267]