Detailed information NTG0000361

Experimentally validated

Overview

• Name: oppD
• Type: Regulator
• Locus tag: SMU_RS01310
• Genome accession: NC_004350
• Coordinates: 247543..248595 (+)
• Length: 350 a.a.
• NCBI ID: WP_002262737.1
• Uniprot ID: Q8DW22
• Organism: Streptococcus mutans UA159
• Function: internalize XIP Competence regulation

Function

Deletion of comR or comS in Streptococcus mutans abolished transformability, as did deletion of the oligopeptide permease subunit oppD, suggesting that XIP is imported.

Genomic Context

Location: 242543..253595

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SMU_RS01295 (SMU.255)	-	243822..245471 (+)	1650	WP_002262740.1	peptide ABC transporter substrate-binding protein	-
SMU_RS01300 (SMU.256)	-	245577..246491 (+)	915	WP_002262739.1	ABC transporter permease	-
SMU_RS01305 (SMU.257)	-	246502..247533 (+)	1032	WP_002262738.1	ABC transporter permease	-
SMU_RS01310 (SMU.258)	oppD	247543..248595 (+)	1053	WP_002262737.1	ABC transporter ATP-binding protein	Regulator
SMU_RS01315 (SMU.259)	amiF	248588..249514 (+)	927	WP_002262736.1	ABC transporter ATP-binding protein	Regulator
SMU_RS01320 (SMU.260)	-	250170..250772 (+)	603	WP_002262735.1	nitroreductase family protein	-
SMU_RS01325 (SMU.261c)	-	250989..251945 (-)	957	WP_002262734.1	helix-turn-helix transcriptional regulator	-
SMU_RS01330 (SMU.262)	ptcA	252184..253203 (+)	1020	WP_002282737.1	putrescine carbamoyltransferase	-

Regulatory network

Positive effect      

Negative effect

Regulator	Target	Regulation
oppD	comS	positive effect
comS	comS	positive effect
comS	comX/sigX	positive effect
comR	comS	positive effect
cipB	comS	positive effect
XrpA	comS	negative effect
comX/sigX	late competence genes	positive effect
scnR	comX/sigX	positive effect
hdrR	comX/sigX	positive effect
mecA	comX/sigX	negative effect
clpC	comX/sigX	negative effect
comR	comX/sigX	positive effect
clpP	comX/sigX	negative effect
XrpA	comX/sigX	negative effect
scnK	comX/sigX	positive effect
comD/blpH	comX/sigX	positive effect
vicK	comX/sigX	negative effect
comE/blpR	comX/sigX	positive effect
scnC	comX/sigX	positive effect
brsR	comX/sigX	positive effect
cipB	comX/sigX	positive effect
vicR	comX/sigX	negative effect
comC/blpC	comX/sigX	positive effect
cipB	comR	positive effect
hdrR	comR	positive effect
XrpA	comR	negative effect
comX/sigX	late competence genes	positive effect
hdrM	hdrR	negative effect
comD/blpH	comE/blpR	positive effect
vicR	comD/blpH	negative effect
comE/blpR	comD/blpH	positive effect
vicK	comD/blpH	negative effect
comC/blpC	comD/blpH	positive effect
vicK	comC/blpC	negative effect
vicK	comE/blpR	negative effect
comE/blpR	comC/blpC	positive effect
comE/blpR	comA/nlmT	positive effect
comE/blpR	comE/blpR	positive effect
comE/blpR	comB/nlmE	positive effect
vicR	comE/blpR	negative effect
brsM	brsR	negative effect
vicR	comC/blpC	negative effect
comA/nlmT	comC/blpC	positive effect
comB/nlmE	comC/blpC	positive effect
ciaH	comC/blpC	positive effect
sepM	comC/blpC	positive effect

Sequence

Protein

• Length: 350 a.a.
• Molecular weight: 39171.64 Da
• Isoelectric Point: 5.5583

>NTDB_id=361 SMU_RS01310 WP_002262737.1 247543..248595(+) (oppD) [Streptococcus mutans UA159]
MSKEKILQVNNLHVNFHTYAGEVKAIRDVSFYLEKGETLAIVGESGSGKSVTTRTLMGLSAKNAEIFGDIEFKGHNLNDL
KEEDWVHIRGNDISMIFQDPMTSLDPTMRIGLQIAEPIIKHEKVTKKEALKRALDMMEKVGIPNAQEHINDYPHQWSGGM
RQRAVIAIALATNPEILVADEPTTALDVTIQAQILHLMKEIQKNTDSSIIFITHDLGVVAGMADRVAVMYAGKIVEYGTV
DEVFYNPQHPYTWGLLNSMPTTNTASGSLHSIPGTPPDLLQPPQGDAFAPRNEFALDIDLKEEPPFFKVSDSHYAATWLL
DDRAPKITPPERILQRWEKWKSLQGEKHND

Nucleotide

• Length: 1053 bp

>NTDB_id=361 SMU_RS01310 WP_002262737.1 247543..248595(+) (oppD) [Streptococcus mutans UA159]
ATGAGTAAGGAAAAAATTTTACAAGTCAATAACCTCCATGTCAATTTTCATACCTATGCAGGTGAAGTAAAAGCTATTCG
CGATGTTAGTTTTTATTTAGAAAAAGGGGAAACGCTGGCTATTGTTGGCGAATCTGGTTCTGGAAAGTCGGTAACCACAC
GAACTTTAATGGGGTTGTCAGCAAAGAATGCTGAGATTTTCGGAGATATTGAGTTTAAGGGACACAATCTAAATGATTTG
AAGGAAGAAGACTGGGTTCATATTCGCGGAAATGATATTTCCATGATTTTCCAAGATCCTATGACAAGTTTGGATCCAAC
CATGCGTATTGGCCTTCAAATTGCTGAACCTATTATCAAGCATGAAAAAGTAACTAAAAAAGAAGCTCTTAAGCGGGCTC
TTGACATGATGGAAAAGGTTGGTATTCCCAATGCGCAAGAACACATCAACGATTATCCTCACCAGTGGTCAGGCGGTATG
CGCCAGCGCGCTGTCATAGCTATTGCTTTGGCAACTAATCCTGAAATCCTTGTTGCAGATGAACCAACGACGGCTCTTGA
TGTTACCATTCAAGCACAAATTCTTCATTTAATGAAAGAAATCCAAAAGAATACTGACTCTTCCATTATCTTTATCACTC
ATGATTTGGGTGTCGTTGCAGGTATGGCAGACCGTGTTGCTGTTATGTATGCAGGCAAGATTGTGGAATATGGTACAGTA
GATGAGGTTTTCTACAACCCACAGCATCCCTACACTTGGGGGCTTCTCAATTCGATGCCTACAACTAATACAGCTTCAGG
CAGTTTGCATTCAATTCCCGGAACACCGCCTGACTTGTTGCAGCCCCCTCAAGGAGATGCTTTTGCTCCAAGAAATGAAT
TCGCTCTTGATATTGATCTTAAGGAGGAGCCGCCATTTTTTAAAGTTAGTGATTCACATTATGCTGCTACTTGGCTGTTA
GATGATCGTGCTCCTAAGATTACTCCGCCGGAAAGAATTTTGCAGCGTTGGGAAAAATGGAAGAGCTTACAGGGAGAAAA
GCATAATGACTGA

Domains

Predicted by InterproScan.

• ABC transporter (ABC_tran) (27-183)

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (235-297)

3D structure

• AlphaFold DB: Q8DW22

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus salivarius strain HSISS4	54.728	99.714	0.546
amiE	Streptococcus thermophilus LMD-9	54.441	99.714	0.543
amiE	Streptococcus thermophilus LMG 18311	54.441	99.714	0.543

References

• [1]: Lauren Mashburn-Warren et al. (2010) A novel double-tryptophan peptide pheromone controls competence in Streptococcus spp. via an Rgg regulator. Molecular Microbiology 78(3):589-606. [PMID: 20969646]