Detailed information NTG0000374

Experimentally validated

Overview

• Name: vicR
• Type: Regulator
• Locus tag: SMU_RS06885
• Genome accession: NC_004350
• Coordinates: 1446201..1446908 (-)
• Length: 235 a.a.
• NCBI ID: WP_002262930.1
• Uniprot ID: -
• Organism: Streptococcus mutans UA159
• Function: repress comCDE expression; repress comX expression Competence regulation

Function

Overexpression of the vic operon caused growth-phase-dependent repression of comC, comDE, and comX.

Genomic Context

Location: 1441201..1451908

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SMU_RS06870 (SMU.1514)	rnc	1443254..1443949 (-)	696	WP_002262927.1	ribonuclease III	-
SMU_RS06875 (SMU.1515)	vicX	1444056..1444859 (-)	804	WP_002262928.1	MBL fold metallo-hydrolase	Regulator
SMU_RS06880 (SMU.1516)	vicK	1444856..1446208 (-)	1353	WP_002262929.1	cell wall metabolism sensor histidine kinase VicK	Regulator
SMU_RS06885 (SMU.1517)	vicR	1446201..1446908 (-)	708	WP_002262930.1	response regulator YycF	Regulator
SMU_RS06890 (SMU.1519)	-	1447261..1448043 (+)	783	WP_002262931.1	amino acid ABC transporter ATP-binding protein	-
SMU_RS06895 (SMU.1520)	-	1448040..1448852 (+)	813	WP_002262932.1	transporter substrate-binding domain-containing protein	-
SMU_RS06900 (SMU.1521)	-	1448870..1449574 (+)	705	WP_002262933.1	amino acid ABC transporter permease	-
SMU_RS06905 (SMU.1522)	-	1449589..1450239 (+)	651	WP_002262934.1	amino acid ABC transporter permease	-
SMU_RS06910 (SMU.1523)	endA	1450307..1451188 (-)	882	WP_002262935.1	DNA/RNA non-specific endonuclease	Machinery gene
SMU_RS06915 (SMU.1524c)	-	1451230..1451418 (-)	189	WP_002262936.1	DNA-directed RNA polymerase subunit beta	-

Regulatory network

Positive effect      

Negative effect

Regulator	Target	Regulation
vicR	comD/blpH	negative effect
comD/blpH	comE/blpR	positive effect
comE/blpR	comD/blpH	positive effect
comE/blpR	comC/blpC	positive effect
comE/blpR	comA/nlmT	positive effect
comE/blpR	comX/sigX	positive effect
comE/blpR	comE/blpR	positive effect
comE/blpR	comB/nlmE	positive effect
vicR	comE/blpR	negative effect
vicK	comE/blpR	negative effect
comD/blpH	comX/sigX	positive effect
comX/sigX	late competence genes	positive effect
scnR	comX/sigX	positive effect
hdrR	comX/sigX	positive effect
mecA	comX/sigX	negative effect
clpC	comX/sigX	negative effect
comR	comX/sigX	positive effect
clpP	comX/sigX	negative effect
XrpA	comX/sigX	negative effect
scnK	comX/sigX	positive effect
comS	comX/sigX	positive effect
vicK	comX/sigX	negative effect
scnC	comX/sigX	positive effect
brsR	comX/sigX	positive effect
cipB	comX/sigX	positive effect
vicR	comX/sigX	negative effect
comC/blpC	comX/sigX	positive effect
vicR	comC/blpC	negative effect
vicK	comD/blpH	negative effect
vicK	comC/blpC	negative effect
comC/blpC	comD/blpH	positive effect
comA/nlmT	comC/blpC	positive effect
comB/nlmE	comC/blpC	positive effect
ciaH	comC/blpC	positive effect
sepM	comC/blpC	positive effect
comX/sigX	late competence genes	positive effect
hdrR	comR	positive effect
hdrM	hdrR	negative effect
comR	comS	positive effect
cipB	comR	positive effect
XrpA	comR	negative effect
XrpA	comS	negative effect
comS	comS	positive effect
oppD	comS	positive effect
cipB	comS	positive effect
brsM	brsR	negative effect

Sequence

Protein

• Length: 235 a.a.
• Molecular weight: 26914.70 Da
• Isoelectric Point: 4.8288

>NTDB_id=374 SMU_RS06885 WP_002262930.1 1446201..1446908(-) (vicR) [Streptococcus mutans UA159]
MKKILIVDDEKPISDIIKFNLAKEGYDTITAFDGREALSKYEEENPDLIILDLMLPELDGLEVAKEVRKNSHVPIIMLSA
KDSEFDKVIGLEIGADDYVTKPFSNRELLARVKAHLRRTENIESAVAEENASGIPEIIIGDLQILPDAFVAKKRGTEVEL
THREFELLHHLATHTGQVMTREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPSRPEYILTRRGVGYYMKSYD

Nucleotide

• Length: 708 bp

>NTDB_id=374 SMU_RS06885 WP_002262930.1 1446201..1446908(-) (vicR) [Streptococcus mutans UA159]
ATGAAGAAAATTCTAATCGTTGACGATGAAAAACCAATTTCTGATATCATCAAGTTTAACTTGGCTAAGGAAGGTTATGA
CACGATTACAGCCTTTGATGGGCGTGAAGCATTAAGTAAATATGAAGAAGAAAATCCTGACTTGATTATATTGGACTTAA
TGTTACCAGAACTAGACGGTCTTGAAGTGGCTAAGGAAGTTAGAAAAAACAGCCATGTTCCAATTATTATGTTATCAGCT
AAAGACAGTGAGTTTGATAAGGTTATTGGTCTTGAAATTGGTGCTGATGATTATGTGACTAAACCTTTTTCTAATCGTGA
ATTACTGGCGCGTGTAAAAGCGCATCTTCGCCGTACTGAAAATATTGAATCCGCAGTGGCTGAGGAAAATGCTTCAGGTA
TACCTGAAATTATTATTGGGGACTTGCAAATCTTACCAGATGCTTTTGTTGCTAAAAAACGTGGAACAGAGGTAGAGTTA
ACTCACCGTGAGTTTGAGTTGTTGCATCACTTAGCGACACACACAGGTCAGGTGATGACGCGTGAACATTTACTTGAAAC
GGTTTGGGGCTATGATTATTTTGGAGATGTCCGTACTGTTGATGTTACTGTTCGTCGTCTGCGTGAAAAAATTGAAGATA
CACCTAGTCGTCCAGAGTACATTTTGACGCGACGCGGTGTTGGTTATTACATGAAATCATATGACTAA

Domains

Predicted by InterproScan.

• Transcriptional regulatory protein, C terminal (Trans_reg_C) (155-230)

• Response regulator receiver domain (Response_reg) (4-113)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
micA	Streptococcus pneumoniae Cp1015	78.541	99.149	0.779
covR	Streptococcus salivarius strain HSISS4	45.923	100	0.467
covR	Lactococcus lactis subsp. lactis strain DGCC12653	45.887	100	0.461
pilG	Acinetobacter baumannii strain A118	42.735	92.126	0.394
pilH	Synechocystis sp. PCC 6803	38.333	99.174	0.38
ciaR	Streptococcus pneumoniae R6	35.622	100	0.371
ciaR	Streptococcus pneumoniae D39	35.622	100	0.371
ciaR	Streptococcus mutans UA159	35.622	100	0.371
ciaR	Streptococcus pneumoniae TIGR4	35.622	100	0.371
ciaR	Streptococcus pneumoniae Rx1	35.622	100	0.371
scnR	Streptococcus mutans UA159	36.207	100	0.367

References

• [1]: D B Senadheera et al. (2012) Regulation of bacteriocin production and cell death by the VicRK signaling system in Streptococcus mutans. Journal of Bacteriology 194(6):1307-16. [PMID: 22228735]
• [2]: M Dilani Senadheera et al. (2007) The Streptococcus mutans vicX gene product modulates gtfB/C expression, biofilm formation, genetic competence, and oxidative stress tolerance. Journal of Bacteriology 189(4):1451-8. [PMID: 17114248]
• [3]: M Dilani Senadheera et al. (2005) A VicRK signal transduction system in Streptococcus mutans affects gtfBCD, gbpB, and ftf expression, biofilm formation, and genetic competence development. Journal of Bacteriology 187(12):4064-76. [PMID: 15937169]