Detailed information NTG0000606

Experimentally validated

Overview

• Name: covR
• Type: Regulator
• Locus tag: V4T04_RS01910
• Genome accession: NZ_JAZICS010000001
• Coordinates: 395011..395703 (-)
• Length: 230 a.a.
• NCBI ID: WP_003130756.1
• Uniprot ID: S6EZK2
• Organism: Lactococcus lactis subsp. lactis strain DGCC12653
• Function: repress the expression of comX Competence regulation

Function

The environmental stress sensor CovRS is a direct repressor of comX.

Genomic Context

Location: 390011..400703

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
V4T04_RS01895	-	391630..391971 (-)	342	WP_010906046.1	P-II family nitrogen regulator	-
V4T04_RS01900	-	392103..393344 (-)	1242	WP_033898637.1	ammonium transporter	-
V4T04_RS01905	covS	393527..395002 (-)	1476	WP_010906048.1	HAMP domain-containing sensor histidine kinase	Regulator
V4T04_RS01910	covR	395011..395703 (-)	693	WP_003130756.1	response regulator transcription factor	Regulator
V4T04_RS01915	-	395859..396389 (-)	531	WP_058219348.1	YceD family protein	-
V4T04_RS01920	-	396520..396765 (-)	246	WP_003130760.1	type B 50S ribosomal protein L31	-
V4T04_RS01925	-	396921..397385 (-)	465	WP_017864799.1	hypothetical protein	-
V4T04_RS01930	-	397467..397829 (-)	363	WP_010906050.1	TIGR02328 family protein	-
V4T04_RS01935	-	398044..398964 (-)	921	WP_349651619.1	CHAP domain-containing protein	-
V4T04_RS01940	-	399077..399913 (-)	837	WP_139917167.1	metallophosphoesterase	-

Regulatory network

Positive effect      

Negative effect

Regulator	Target	Regulation
covR	comX	negative effect
clpP	comX	negative effect
mecA	comX	negative effect
ccpA	comX	negative effect
codY	comX	negative effect
covS	comX	negative effect
clpC	comX	negative effect

Sequence

Protein

• Length: 230 a.a.
• Molecular weight: 26684.36 Da
• Isoelectric Point: 5.1273

>NTDB_id=606 V4T04_RS01910 WP_003130756.1 395011..395703(-) (covR) [Lactococcus lactis subsp. lactis strain DGCC12653]
MTSKKILIIEDEKNLARFVSLELEHEGYATEIKDNGRSGLEEATSKDYDLILLDLMLPELDGFEVARRLRKEKDTPIIMM
TARDSTMDRVAGLDIGADDYITKPFAIEELLARVRAFFRREEHGHAVERAENTSFRDLVIDKTNRTVHRGKKVIDLTRRE
YDLLLTLMQNVGDVVTREHLVSQVWGYEEGTETNVVDVYIRYLRNKIDVEGQDSYIQTVRGLGYVMRERK

Nucleotide

• Length: 693 bp

>NTDB_id=606 V4T04_RS01910 WP_003130756.1 395011..395703(-) (covR) [Lactococcus lactis subsp. lactis strain DGCC12653]
ATGACTTCAAAGAAAATTTTGATTATTGAGGATGAAAAGAATTTAGCGCGATTCGTCTCATTAGAATTAGAACATGAAGG
CTATGCGACTGAGATTAAAGATAATGGTCGTTCTGGTCTTGAAGAAGCAACTTCAAAAGATTATGATTTAATCTTGCTTG
ATTTGATGCTTCCTGAACTTGACGGTTTTGAGGTAGCCCGTCGTTTACGAAAAGAAAAAGATACACCAATCATTATGATG
ACGGCACGTGATTCAACGATGGACCGTGTTGCTGGTCTTGATATTGGGGCTGATGATTATATCACTAAACCTTTTGCGAT
TGAGGAACTTTTGGCGCGTGTTCGTGCTTTCTTCCGTCGTGAAGAGCATGGTCATGCCGTTGAACGTGCTGAAAATACTT
CTTTCCGTGATCTTGTGATTGACAAAACGAATCGCACAGTTCATCGTGGTAAAAAAGTAATTGATTTAACACGTCGTGAG
TATGACCTCCTTTTGACATTGATGCAAAATGTTGGCGATGTTGTGACTCGTGAACATTTAGTTTCACAAGTTTGGGGATA
TGAAGAAGGAACAGAAACAAATGTTGTGGATGTTTATATTCGTTATCTTAGAAATAAAATTGATGTTGAAGGTCAAGATA
GCTATATTCAAACTGTTCGTGGCTTAGGTTATGTAATGCGTGAACGCAAATAA

Domains

Predicted by InterproScan.

• Response regulator receiver domain (Response_reg) (6-115)

• Transcriptional regulatory protein, C terminal (Trans_reg_C) (153-226)

3D structure

• AlphaFold DB: S6EZK2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
covR	Streptococcus salivarius strain HSISS4	68.421	99.563	0.681
vicR	Streptococcus mutans UA159	45.887	100	0.461
micA	Streptococcus pneumoniae Cp1015	43.478	100	0.435
ciaR	Streptococcus mutans UA159	37.611	100	0.379
ciaR	Streptococcus pneumoniae Rx1	36.726	100	0.371
ciaR	Streptococcus pneumoniae D39	36.726	100	0.371
ciaR	Streptococcus pneumoniae R6	36.726	100	0.371
ciaR	Streptococcus pneumoniae TIGR4	36.726	100	0.371

References

• [1]: Frédéric Toussaint et al. (2024) Unveiling the regulatory network controlling natural transformation in lactococci. PLoS Genetics 20(7):e1011340. [PMID: 38950059]