Detailed information    

experimental Experimentally validated

Overview

Name   covS   Type   Regulator
Locus tag   V4T04_RS01905 Genome accession   NZ_JAZICS010000001
Coordinates   393527..395002 (-) Length   491 a.a.
NCBI ID   WP_010906048.1    Uniprot ID   A0A2A9ICZ0
Organism   Lactococcus lactis subsp. lactis strain DGCC12653     
Function   repress the expression of comX   
Competence regulation

Function

The environmental stress sensor CovRS is a direct repressor of comX.


Genomic Context

Location: 388527..400002
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V4T04_RS01890 murD 389786..391138 (-) 1353 WP_003130748.1 UDP-N-acetylmuramoyl-L-alanine--D-glutamate ligase -
  V4T04_RS01895 - 391630..391971 (-) 342 WP_010906046.1 P-II family nitrogen regulator -
  V4T04_RS01900 - 392103..393344 (-) 1242 WP_033898637.1 ammonium transporter -
  V4T04_RS01905 covS 393527..395002 (-) 1476 WP_010906048.1 HAMP domain-containing sensor histidine kinase Regulator
  V4T04_RS01910 covR 395011..395703 (-) 693 WP_003130756.1 response regulator transcription factor Regulator
  V4T04_RS01915 - 395859..396389 (-) 531 WP_058219348.1 YceD family protein -
  V4T04_RS01920 - 396520..396765 (-) 246 WP_003130760.1 type B 50S ribosomal protein L31 -
  V4T04_RS01925 - 396921..397385 (-) 465 WP_017864799.1 hypothetical protein -
  V4T04_RS01930 - 397467..397829 (-) 363 WP_010906050.1 TIGR02328 family protein -
  V4T04_RS01935 - 398044..398964 (-) 921 WP_349651619.1 CHAP domain-containing protein -
  V4T04_RS01940 - 399077..399913 (-) 837 WP_139917167.1 metallophosphoesterase -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  covS comX negative effect
  clpP comX negative effect
  mecA comX negative effect
  ccpA comX negative effect
  codY comX negative effect
  covR comX negative effect
  clpC comX negative effect

Sequence

Protein


Download         Length: 491 a.a.        Molecular weight: 56195.08 Da        Isoelectric Point: 4.8253

>NTDB_id=607 V4T04_RS01905 WP_010906048.1 393527..395002(-) (covS) [Lactococcus lactis subsp. lactis strain DGCC12653]
MKKLFTFNKKKETVEESSAKRSIMLRWAFANTVFCFITFTLFATLTYQLTISSFIKEEQQLLTRSMDSVEEVLEKADAPL
NSSNLNTYIEATSKIQNGESEGMSLGSIIGTRKAFYIYDLNHKLLYSTNRHTFGFQNQANNEMKEIRGENPGYLVQRKII
SKSTGQVVGYLQAFYDTTTYHRISNLLLIVLLILEIVALIVAQLIGYFMANYFMKPLEKLYQGMQEMANDPTNDFEPIEI
QSGDEIEELAHVYNDMMLKMKAYLEQQNRFVSDVSHELRTPLAVLDGHINLLNRWGKNDPEVLDESLQASLDEVDRMKKM
LEEMLALARLENVDLSSEELDCDVGKVCNRALKNFQLLHDDFEIVLDNRLIYPTHARISENHFEQGLRILLDNAAKYSPD
DRKEIVITVSEDEQFVITSVSDKGIGISEEDINHLFERFFRADKARNREIGGTGLGLSILARLAENYQGEIEVNSELGLG
STFTLKFPKIK

Nucleotide


Download         Length: 1476 bp        

>NTDB_id=607 V4T04_RS01905 WP_010906048.1 393527..395002(-) (covS) [Lactococcus lactis subsp. lactis strain DGCC12653]
ATGAAAAAATTATTTACTTTCAATAAGAAAAAAGAGACGGTAGAGGAAAGTTCGGCTAAGCGGTCTATCATGCTTAGGTG
GGCTTTTGCTAACACCGTTTTTTGTTTTATTACTTTTACTTTGTTTGCTACTTTGACTTATCAATTGACAATAAGTTCTT
TTATTAAAGAAGAACAACAATTATTAACTCGCTCTATGGATAGTGTTGAGGAAGTCTTAGAAAAAGCAGATGCTCCTTTG
AATTCATCGAATTTGAACACATATATTGAAGCGACATCAAAGATTCAAAATGGTGAGTCTGAAGGAATGAGTTTGGGTAG
TATCATTGGGACACGGAAAGCATTTTATATTTATGACTTAAATCATAAATTATTGTATTCAACGAATCGACATACTTTTG
GTTTTCAGAATCAGGCTAATAATGAAATGAAAGAGATTCGGGGAGAAAATCCAGGTTATTTAGTTCAGAGAAAAATTATT
TCTAAGTCAACAGGACAAGTTGTGGGATACTTACAGGCTTTTTATGATACGACGACTTATCATCGAATTTCTAATCTTCT
TTTGATTGTCTTGTTAATTTTAGAAATTGTTGCCTTGATTGTCGCTCAGTTGATTGGTTATTTCATGGCGAATTACTTTA
TGAAACCATTGGAAAAACTTTATCAGGGCATGCAAGAAATGGCAAATGATCCAACAAATGATTTTGAACCGATTGAAATT
CAAAGTGGAGATGAAATCGAAGAGTTGGCTCATGTTTACAATGATATGATGTTGAAGATGAAGGCATATCTTGAACAGCA
AAATCGCTTTGTTTCTGATGTTTCTCATGAATTAAGAACACCTTTAGCTGTCTTGGATGGACATATTAACTTGTTGAATC
GATGGGGGAAAAATGATCCAGAAGTATTAGATGAATCTTTGCAGGCAAGTTTAGATGAAGTTGATCGAATGAAGAAAATG
CTTGAGGAGATGTTGGCTCTTGCTCGCCTTGAAAATGTTGACTTATCAAGTGAAGAACTGGATTGTGATGTTGGAAAAGT
TTGTAATCGGGCCCTTAAGAATTTTCAGCTTTTGCATGATGATTTTGAAATAGTGCTTGATAACCGGCTTATTTATCCCA
CTCATGCGCGAATTTCAGAAAATCATTTTGAACAAGGTTTACGAATTCTGTTAGATAATGCTGCTAAATACTCTCCTGAT
GATCGTAAAGAAATCGTGATTACTGTTTCTGAGGATGAACAGTTTGTGATTACGAGTGTATCTGACAAAGGAATTGGAAT
TTCAGAAGAAGATATTAATCATCTTTTTGAGCGTTTCTTTAGAGCGGACAAGGCTCGCAATCGAGAAATTGGTGGAACTG
GTTTAGGTTTATCAATTTTAGCTCGATTGGCAGAAAATTATCAAGGGGAAATTGAGGTAAATTCAGAACTGGGTCTTGGT
TCAACTTTTACACTAAAATTCCCGAAAATAAAATAA

Domains

Predicted by InterproScan.

ArlS sensor domain (ArlS_N)

(54-185)

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(380-489)

HAMP domain (HAMP)

(208-258)

His Kinase A (phospho-acceptor) domain (HisKA)

(267-332)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2A9ICZ0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Frédéric Toussaint et al. (2024) Unveiling the regulatory network controlling natural transformation in lactococci. PLoS Genetics 20(7):e1011340. [PMID: 38950059]