Detailed information    

experimental Experimentally validated

Overview

Name   ccpA   Type   Regulator
Locus tag   V4T04_RS04035 Genome accession   NZ_JAZICS010000003
Coordinates   18361..19359 (-) Length   332 a.a.
NCBI ID   WP_003129447.1    Uniprot ID   A0A2A9IN00
Organism   Lactococcus lactis subsp. lactis strain DGCC12653     
Function   repress the expression of comX   
Competence regulation

Function

The carbon catabolite regulator CcpA is a direct repressor of comX.


Genomic Context

Location: 13361..24359
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V4T04_RS04015 trxA 13751..14065 (-) 315 WP_003129442.1 thioredoxin -
  V4T04_RS04020 - 14162..16492 (-) 2331 WP_349651723.1 endonuclease MutS2 -
  V4T04_RS04025 - 16537..17097 (-) 561 WP_003129445.1 CvpA family protein -
  V4T04_RS04030 - 17257..18222 (-) 966 WP_023189681.1 NAD(P)/FAD-dependent oxidoreductase -
  V4T04_RS04035 ccpA 18361..19359 (-) 999 WP_003129447.1 catabolite control protein A Regulator
  V4T04_RS04040 - 19574..20662 (+) 1089 WP_058203077.1 M24 family metallopeptidase -
  V4T04_RS04045 - 20829..22832 (-) 2004 WP_349651724.1 cell division site-positioning protein MapZ family protein -
  V4T04_RS04050 - 22979..24133 (-) 1155 WP_349651725.1 THUMP domain-containing class I SAM-dependent RNA methyltransferase -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  ccpA comX negative effect
  clpP comX negative effect
  mecA comX negative effect
  codY comX negative effect
  covR comX negative effect
  covS comX negative effect
  clpC comX negative effect

Sequence

Protein


Download         Length: 332 a.a.        Molecular weight: 36602.71 Da        Isoelectric Point: 4.9196

>NTDB_id=604 V4T04_RS04035 WP_003129447.1 18361..19359(-) (ccpA) [Lactococcus lactis subsp. lactis strain DGCC12653]
MVESTTTIYDVARVAGVSMATVSRVVNGNANVKEKTRQKVLEAIAELDYRPNAVARGLASKRTTTVGVILPTITSTYFAA
ITRGVDDIASMYKYNMILANSDNDVEKEEKVLETFLSKQVDGIVYMGSSLDEKIRTSLKNSRTPVVLVGTIDGDKEIPSV
NIDYHLAAYQSTKKLIDSGNKKIAYIMGSLKDVENTERMVGYQEALLEANIEFDENLVFEGNYSYEQGKALAERLLERGA
TSAVVSHDTVAVGLLSAMMDKGVKVPEDFEIISGANSPITQYTYPTLTSVNQPLYDLGAVAMRLLTKLMLKEDVEQNQLV
LDHEIFSRRSTK

Nucleotide


Download         Length: 999 bp        

>NTDB_id=604 V4T04_RS04035 WP_003129447.1 18361..19359(-) (ccpA) [Lactococcus lactis subsp. lactis strain DGCC12653]
ATGGTAGAATCAACAACAACAATTTATGATGTGGCACGTGTCGCCGGAGTGTCAATGGCAACCGTTAGTCGTGTTGTAAA
TGGAAATGCAAATGTAAAGGAAAAGACGCGCCAGAAGGTCTTAGAAGCTATTGCTGAGCTTGACTATCGTCCTAATGCAG
TTGCGCGCGGACTCGCAAGTAAACGTACAACAACAGTTGGTGTTATCTTGCCAACCATCACTTCAACTTACTTCGCAGCG
ATTACTCGCGGGGTTGATGATATCGCTTCCATGTATAAATACAACATGATTTTAGCTAATAGTGATAATGATGTTGAAAA
AGAAGAAAAAGTTTTAGAAACTTTCTTATCAAAACAAGTTGACGGAATCGTCTATATGGGTTCATCTTTAGATGAAAAAA
TTAGAACTTCCCTCAAAAATTCAAGAACACCTGTCGTTTTAGTTGGAACAATCGATGGAGATAAAGAAATTCCATCTGTT
AATATTGATTACCATTTGGCTGCTTATCAATCAACTAAAAAATTGATTGATAGTGGAAATAAAAAAATCGCTTATATCAT
GGGTTCATTGAAAGACGTTGAAAATACAGAACGTATGGTTGGTTATCAAGAAGCTTTGCTTGAAGCAAATATTGAATTTG
ATGAAAACCTCGTTTTTGAAGGTAATTATAGCTATGAACAAGGAAAAGCACTTGCTGAACGTTTACTTGAGCGAGGAGCA
ACTTCTGCGGTAGTATCACATGATACAGTAGCCGTTGGACTCTTGTCTGCAATGATGGATAAAGGAGTGAAAGTTCCTGA
AGATTTCGAAATTATCTCAGGTGCAAATTCACCAATTACTCAATATACATATCCAACTTTAACTTCTGTTAACCAACCCC
TTTACGATTTGGGAGCAGTAGCAATGCGTCTTTTGACAAAATTAATGCTTAAAGAAGATGTTGAACAAAATCAATTAGTT
TTGGATCATGAAATCTTTTCTCGTCGTTCTACCAAATAA

Domains

Predicted by InterproScan.

Bacterial regulatory proteins, lacI family (LacI)

(7-52)

Periplasmic binding protein-like domain (Peripla_BP_3)

(173-331)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2A9IN00

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccpA Streptococcus pneumoniae D39

57.402

99.699

0.572
  ccpA Streptococcus gordonii str. Challis substr. CH1

57.1

99.699

0.569

Multiple sequence alignment    



References

[1] Frédéric Toussaint et al. (2024) Unveiling the regulatory network controlling natural transformation in lactococci. PLoS Genetics 20(7):e1011340. [PMID: 38950059]