Detailed information NTG0000428

Experimentally validated

Overview

• Name: ccpA
• Type: Regulator
• Locus tag: SGO_RS03795
• Genome accession: NC_009785
• Coordinates: 812014..813018 (+)
• Length: 334 a.a.
• NCBI ID: WP_012000235.1
• Uniprot ID: Q3Y532
• Organism: Streptococcus gordonii str. Challis substr. CH1
• Function: require for competence development Competence regulation

Function

A ccpA mutant was severely impaired in its biofilm-forming ability, showed a defect in extracellular polysaccharide production and reduced competence.

Genomic Context

Location: 807014..818018

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SGO_RS03775 (SGO_0767)	-	807340..808368 (-)	1029	WP_041789612.1	ABC transporter substrate-binding protein	-
SGO_RS03780 (SGO_0769)	-	808513..809268 (-)	756	WP_012000231.1	ABC transporter ATP-binding protein	-
SGO_RS03785 (SGO_0770)	-	809270..810316 (-)	1047	WP_012000232.1	FecCD family ABC transporter permease	-
SGO_RS11030	-	810609..810716 (+)	108	Protein_749	DNA-entry nuclease	-
SGO_RS03790 (SGO_0771)	-	810767..811849 (-)	1083	WP_012000233.1	M24 family metallopeptidase	-
SGO_RS03795 (SGO_0773)	ccpA	812014..813018 (+)	1005	WP_012000235.1	catabolite control protein A	Regulator
SGO_RS03800 (SGO_0774)	-	813163..814161 (+)	999	WP_041789613.1	glycosyltransferase family 4 protein	-
SGO_RS03805 (SGO_0775)	-	814163..815476 (+)	1314	WP_012000237.1	glycosyltransferase family 4 protein	-
SGO_RS03810 (SGO_0776)	-	815512..815874 (+)	363	WP_012000238.1	hypothetical protein	-
SGO_RS11035 (SGO_0777)	-	816208..816579 (+)	372	WP_012000239.1	hypothetical protein	-

Sequence

Protein

• Length: 334 a.a.
• Molecular weight: 37022.13 Da
• Isoelectric Point: 6.5109

>NTDB_id=428 SGO_RS03795 WP_012000235.1 812014..813018(+) (ccpA) [Streptococcus gordonii str. Challis substr. CH1]
MNTDDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEVIDRLDYRPNAVARGLASKKTTTVGVVIPSITNSYFS
TLAKGIDDIAEMYKYNIVLANSDEDDDKEVSVVNTLFSKQVDGIIFMGYHLTEKIRSEFSRSRTPVVLAGTIDVEHQLPS
VNIDYKQATIDAITFLAKRNKKIAFVCGPLVDDINGKVRLSGYKTALKSKKLSYSEGLVFESKYGYDDGYNLAERVIASK
ATAAFVTGDELAAGLLNGLADCGVKIPEDFEIITSDDSQISRYTRPNLSTISQPLYDLGAISMRMLTKIMHKEELEEREV
VLSHGIYERNSTRK

Nucleotide

• Length: 1005 bp

>NTDB_id=428 SGO_RS03795 WP_012000235.1 812014..813018(+) (ccpA) [Streptococcus gordonii str. Challis substr. CH1]
ATGAACACAGACGATACAGTAACCATTTATGATGTCGCCCGTGAAGCAGGGGTTTCTATGGCGACAGTTAGTCGTGTAGT
TAATGGAAACAAGAATGTAAAAGAAAACACTCGGAAAAAAGTTTTGGAAGTAATTGATCGCCTTGATTATCGTCCAAATG
CAGTAGCTCGTGGTTTGGCTAGCAAGAAGACTACGACAGTTGGTGTTGTGATTCCAAGTATTACAAATAGTTATTTCTCA
ACACTCGCCAAGGGAATTGATGATATTGCTGAGATGTATAAATACAATATCGTCCTAGCAAACAGCGATGAGGATGATGA
TAAGGAAGTTTCAGTTGTAAACACTCTCTTTTCGAAACAAGTAGATGGTATTATCTTTATGGGTTACCACCTTACTGAAA
AGATTCGGTCAGAATTTTCACGCTCACGCACGCCAGTTGTCTTGGCTGGAACTATTGATGTGGAGCACCAATTGCCAAGT
GTTAATATAGATTATAAACAGGCTACAATTGATGCTATCACATTCCTTGCTAAGCGCAATAAAAAAATTGCTTTTGTGTG
CGGACCTTTAGTTGATGATATCAATGGAAAAGTACGCTTATCCGGTTATAAAACGGCTCTTAAGAGCAAAAAGCTGTCAT
ACAGCGAAGGTCTTGTTTTTGAGTCTAAGTATGGCTATGATGATGGCTATAATTTAGCTGAACGAGTAATTGCTTCTAAA
GCAACGGCTGCTTTTGTTACAGGTGATGAGCTGGCAGCTGGTCTTTTAAACGGACTAGCTGATTGCGGAGTAAAAATTCC
AGAGGATTTTGAAATTATTACTAGCGATGATTCACAAATATCTCGCTATACACGTCCAAATTTGTCGACTATAAGCCAGC
CTTTATATGATTTAGGTGCTATTAGTATGCGTATGCTGACTAAAATTATGCACAAGGAAGAGTTGGAAGAACGTGAAGTT
GTTCTATCTCATGGTATTTACGAACGGAACTCAACTAGAAAATAA

Domains

Predicted by InterproScan.

• Bacterial regulatory proteins, lacI family (LacI) (8-53)

• Periplasmic binding protein-like domain (Peripla_BP_3) (175-332)

3D structure

• AlphaFold DB: Q3Y532

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ccpA	Streptococcus pneumoniae D39	86.826	100	0.868
ccpA	Lactococcus lactis subsp. lactis strain DGCC12653	57.1	99.699	0.569

References

• [1]: L Zheng et al. (2012) CcpA regulates biofilm formation and competence in Streptococcus gordonii. Molecular Oral Microbiology 27(2):83-94. [PMID: 22394467]