Detailed information NTG0000240

Experimentally validated

Overview

• Name: ccpA
• Type: Regulator
• Locus tag: SPD_RS09570
• Genome accession: NC_008533
• Coordinates: 1790860..1791870 (-)
• Length: 336 a.a.
• NCBI ID: WP_000990602.1
• Uniprot ID: A0A0H2ZQ65
• Organism: Streptococcus pneumoniae D39
• Function: regulate comCDE transcription and transformation Competence regulation

Function

CcpA binds to the cis-acting catabolite response elements (cre) in the comCDE promoter region to regulate comCDE transcription and transformation. Moreover, CcpA can coregulate comCDE transcription with phosphorylated and dephosphorylated ComE. Regulation of comCDE transcription and transformation by CcpA was also affected by carbon source signals.

Genomic Context

Location: 1785860..1796870

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SPD_RS09540 (SPD_1791)	-	1785880..1787094 (-)	1215	WP_000666469.1	pyridoxal phosphate-dependent aminotransferase	-
SPD_RS09545 (SPD_1792)	-	1787250..1787543 (-)	294	WP_000977365.1	hypothetical protein	-
SPD_RS09550 (SPD_1793)	-	1787705..1788157 (+)	453	WP_000079154.1	universal stress protein	-
SPD_RS09555 (SPD_1794)	-	1788192..1789580 (-)	1389	WP_000453139.1	Cof-type HAD-IIB family hydrolase	-
SPD_RS09560 (SPD_1796)	-	1789646..1790608 (+)	963	WP_001124777.1	asparaginase	-
SPD_RS09565 (SPD_1795)	-	1790605..1790796 (-)	192	WP_001092029.1	hypothetical protein	-
SPD_RS09570 (SPD_1797)	ccpA	1790860..1791870 (-)	1011	WP_000990602.1	catabolite control protein A	Regulator
SPD_RS09575 (SPD_1798)	-	1792295..1792894 (-)	600	WP_000866719.1	response regulator transcription factor	-
SPD_RS09580 (SPD_1799)	-	1792896..1793993 (-)	1098	WP_000893546.1	sensor histidine kinase	-
SPD_RS09585 (SPD_1800)	-	1793993..1794730 (-)	738	WP_000832834.1	ABC transporter permease	-
SPD_RS09590 (SPD_1801)	-	1794732..1795616 (-)	885	WP_000219348.1	ABC transporter ATP-binding protein	-
SPD_RS09595 (SPD_1802)	-	1795603..1795797 (-)	195	WP_000820180.1	hypothetical protein	-
SPD_RS09600 (SPD_1803)	-	1795794..1796000 (-)	207	WP_000503437.1	hypothetical protein	-

Regulatory network

Positive effect      

Negative effect

Regulator	Target	Regulation
ccpA	comC/comC1	positive effect
comC/comC1	comD/comD1	positive effect
comD/comD1	comE	positive effect
comE	comM	positive effect
comM	cbpD	negative effect
cbpD	lytA	positive effect
cbpD	lytC	positive effect
comE	comW	positive effect
comW	comX/comX1	positive effect
comW	comX/comX2	positive effect
mecA	comW	negative effect
clpC	comW	negative effect
clpP	comW	negative effect
comE	comA	positive effect
comA	comC/comC1	positive effect
comE	comB	positive effect
comB	comC/comC1	positive effect
comE	comE	positive effect
comE	comX/comX1	positive effect
comE	comD/comD1	positive effect
comE	comX/comX2	positive effect
comE	comC/comC1	positive effect
stkP	comE	positive effect
ccpA	comE	positive effect
comX/comX1	late competence genes	positive effect
clpE	comX/comX1	negative effect
clpP	comX/comX1	negative effect
ccpA	comD/comD1	positive effect
comX/comX2	late competence genes	positive effect
clpE	comX/comX2	negative effect
clpP	comX/comX2	negative effect
htrA	comC/comC1	negative effect
ciaR	comC/comC1	negative effect
ciaH	comC/comC1	negative effect
comX/comX2	late competence genes	positive effect
comX/comX1	late competence genes	positive effect
htrA	comEA/celA/cilE	negative effect
htrA	comEC/celB	negative effect
ciaR	htrA	positive effect
ciaH	htrA	positive effect

Sequence

Protein

• Length: 336 a.a.
• Molecular weight: 37042.99 Da
• Isoelectric Point: 5.3212

>NTDB_id=240 SPD_RS09570 WP_000990602.1 1790860..1791870(-) (ccpA) [Streptococcus pneumoniae D39]
MNADDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEVIDRLDYRPNAVARGLASKKTATVGVVIPNITNGYFS
SLAKGIDDIAEMYKYNIVLANSDEDNEKEVSVVNTLFSKQVDGIIYMGYHLTDKIRSEFSRSRTPIVLAGTVDVEHQLPS
VNIDYKQATIDAVSYLAKENERIAFVSGPLVDDINGKVRLVGYKETLKKAGITYSEGLVFESKYSYDDGYALAERLISSN
ATAAVVTGDELAAGVLNGLADKGVSVPEDFEIITSDDSQISRFTRPNLTTIAQPLYDLGAISMRMLTKIMHKEELEEREV
LLPHGLTERSSTRKRK

Nucleotide

• Length: 1011 bp

>NTDB_id=240 SPD_RS09570 WP_000990602.1 1790860..1791870(-) (ccpA) [Streptococcus pneumoniae D39]
ATGAATGCAGATGATACAGTAACCATTTATGATGTCGCTCGTGAAGCAGGTGTTTCCATGGCGACGGTCAGCCGTGTGGT
CAATGGCAATAAAAATGTAAAAGAGAATACCCGTAAAAAAGTGCTAGAGGTAATTGATCGTTTGGATTATCGTCCAAATG
CAGTTGCGCGTGGTCTTGCAAGTAAAAAGACAGCCACTGTCGGTGTCGTGATTCCAAATATTACCAATGGTTATTTTTCG
AGTTTGGCTAAGGGGATTGATGATATTGCAGAAATGTACAAGTACAATATTGTCCTAGCTAATAGCGATGAAGATAACGA
GAAAGAAGTTTCTGTTGTCAATACCCTCTTTTCAAAGCAGGTAGATGGCATTATCTATATGGGTTATCACTTGACAGATA
AAATTCGCTCAGAATTTTCGCGTTCACGTACTCCGATTGTTCTCGCAGGAACTGTCGATGTTGAGCACCAGTTGCCAAGT
GTCAATATTGACTATAAGCAAGCAACAATTGATGCAGTGAGTTACCTTGCTAAAGAAAATGAGCGTATTGCTTTCGTTAG
CGGTCCGCTAGTGGATGACATCAATGGTAAGGTTCGTTTAGTTGGCTACAAGGAAACCTTGAAAAAAGCAGGAATCACTT
ATAGTGAGGGTTTGGTATTTGAATCTAAATATAGCTATGATGATGGTTACGCCTTAGCAGAGCGTTTGATTTCATCAAAT
GCAACTGCAGCAGTTGTGACAGGTGATGAGTTGGCAGCAGGAGTCTTGAACGGTTTGGCTGATAAAGGTGTTTCTGTGCC
AGAAGATTTTGAAATTATTACTAGTGATGATTCACAAATCTCACGCTTTACCCGTCCAAACTTGACAACGATTGCCCAAC
CTCTTTATGACCTTGGTGCTATTAGTATGCGTATGTTGACCAAGATTATGCATAAGGAAGAGTTGGAAGAACGTGAAGTT
CTCTTACCTCATGGTTTGACAGAACGTAGCTCAACACGAAAACGTAAATAG

Domains

Predicted by InterproScan.

• Bacterial regulatory proteins, lacI family (LacI) (8-53)

• Periplasmic binding protein-like domain (Peripla_BP_3) (181-332)

3D structure

• AlphaFold DB: A0A0H2ZQ65

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ccpA	Streptococcus gordonii str. Challis substr. CH1	86.826	100	0.868
ccpA	Lactococcus lactis subsp. lactis strain DGCC12653	57.402	99.699	0.572

References

• [1]: Yapeng Zhang et al. (2023) comCDE (Competence) Operon Is Regulated by CcpA in Streptococcus pneumoniae D39. Microbiology Spectrum 11(3):e0001223. [PMID: 37036382]