Detailed information NTG0000216

Experimentally validated

Overview

• Name: stkP
• Type: Regulator
• Locus tag: SPD_RS08205
• Genome accession: NC_008533
• Coordinates: 1563636..1565615 (-)
• Length: 659 a.a.
• NCBI ID: WP_000614552.1
• Uniprot ID: Q8DNS0
• Organism: Streptococcus pneumoniae D39
• Function: phosphorylate ComE Competence regulation

Function

StkP is involved in the regulation of cell wall synthesis and cell division/elongation in S. pneumoniae. A stkP mutant displays a strongly reduced natural transformability compared to wild type. StkP could phosphorylate ComE at Thr128 and the StkP/ComE pathway induced global changes in response to acidic stress.

Genomic Context

Location: 1558636..1570615

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SPD_RS08175 (SPD_1536)	-	1558731..1560005 (-)	1275	WP_000704388.1	hydroxymethylglutaryl-CoA reductase, degradative	-
SPD_RS08180 (SPD_1537)	-	1560005..1561177 (-)	1173	WP_000151964.1	hydroxymethylglutaryl-CoA synthase	-
SPD_RS08185 (SPD_1538)	-	1561308..1561556 (-)	249	WP_001818278.1	hypothetical protein	-
SPD_RS08190 (SPD_1539)	-	1561624..1562250 (-)	627	Protein_1549	transposase family protein	-
SPD_RS08195 (SPD_1540)	-	1562312..1562761 (-)	450	WP_000176924.1	threonine/serine exporter family protein	-
SPD_RS08200 (SPD_1541)	-	1562763..1563521 (-)	759	WP_000392880.1	threonine/serine exporter family protein	-
SPD_RS08205 (SPD_1542)	stkP	1563636..1565615 (-)	1980	WP_000614552.1	serine/threonine-protein kinase StkP	Regulator
SPD_RS08210 (SPD_1543)	-	1565612..1566352 (-)	741	WP_000406247.1	Stp1/IreP family PP2C-type Ser/Thr phosphatase	-
SPD_RS08215 (SPD_1544)	rsmB	1566367..1567680 (-)	1314	WP_001808992.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
SPD_RS08220 (SPD_1545)	fmt	1567673..1568608 (-)	936	WP_000163696.1	methionyl-tRNA formyltransferase	-

Regulatory network

Positive effect      

Negative effect

Regulator	Target	Regulation
stkP	comE	positive effect
comE	comM	positive effect
comM	cbpD	negative effect
cbpD	lytA	positive effect
cbpD	lytC	positive effect
comE	comW	positive effect
comW	comX/comX1	positive effect
comW	comX/comX2	positive effect
mecA	comW	negative effect
clpC	comW	negative effect
clpP	comW	negative effect
comE	comA	positive effect
comA	comC/comC1	positive effect
comE	comB	positive effect
comB	comC/comC1	positive effect
comE	comE	positive effect
comE	comX/comX1	positive effect
comE	comD/comD1	positive effect
comE	comX/comX2	positive effect
comE	comC/comC1	positive effect
comD/comD1	comE	positive effect
ccpA	comE	positive effect
comX/comX1	late competence genes	positive effect
clpE	comX/comX1	negative effect
clpP	comX/comX1	negative effect
comC/comC1	comD/comD1	positive effect
ccpA	comD/comD1	positive effect
comX/comX2	late competence genes	positive effect
clpE	comX/comX2	negative effect
clpP	comX/comX2	negative effect
ccpA	comC/comC1	positive effect
htrA	comC/comC1	negative effect
ciaR	comC/comC1	negative effect
ciaH	comC/comC1	negative effect
comX/comX2	late competence genes	positive effect
comX/comX1	late competence genes	positive effect
htrA	comEA/celA/cilE	negative effect
htrA	comEC/celB	negative effect
ciaR	htrA	positive effect
ciaH	htrA	positive effect

Sequence

Protein

• Length: 659 a.a.
• Molecular weight: 72292.76 Da
• Isoelectric Point: 8.9204

>NTDB_id=216 SPD_RS08205 WP_000614552.1 1563636..1565615(-) (stkP) [Streptococcus pneumoniae D39]
MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVLRTNYQTDPIAVARFQREARAMADLDHPHIVRITDIG
EEDGQQYLAMEYVAGLDLKRYIKEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVA
FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAVTIALQHFQNPLPSVIAENSSVP
QALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYNRRNESKLIFDETSKADTKTLPKVSQSTLTSIPKVQAQTEHKSIK
NPSQAVTEETYQPQAPKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANFEIGEEKTE
ASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSDVIAELKEKKVPDNLIKIEEEESNESEAGTV
LKQSLPEGTTYDLSKATQIVLTVAKKATTIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAG
TTYDVSKPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSVEGMVVEQSPRAGEKVDL
NKTRVKISIYKPKTTSATP

Nucleotide

• Length: 1980 bp

>NTDB_id=216 SPD_RS08205 WP_000614552.1 1563636..1565615(-) (stkP) [Streptococcus pneumoniae D39]
ATGATCCAAATCGGCAAGATTTTTGCCGGACGCTATCGGATTGTCAAACAGATTGGTCGAGGAGGCATGGCGGATGTCTA
CCTAGCCAAAGACTTAATCTTAGATGGGGAAGAAGTGGCAGTGAAGGTTCTGAGGACCAACTACCAGACGGACCCGATAG
CTGTAGCTCGTTTTCAGCGTGAAGCGAGAGCTATGGCAGATCTAGACCATCCTCATATCGTTCGGATAACAGATATTGGT
GAGGAAGACGGTCAACAGTATCTTGCAATGGAGTATGTTGCTGGACTAGACCTCAAACGCTATATCAAGGAACATTATCC
TCTTTCTAATGAAGAAGCAGTCCGTATCATGGGACAAATTCTCTTGGCTATGCGCTTGGCCCATACTCGAGGAATTGTTC
ACAGGGACTTGAAACCTCAAAATATCCTTTTGACACCAGATGGGACTGCCAAGGTCACAGACTTTGGGATTGCTGTAGCC
TTTGCAGAGACAAGTCTGACCCAGACTAACTCGATGTTGGGCTCAGTTCATTACTTGTCACCAGAGCAGGCGCGTGGTTC
GAAGGCGACTGTGCAGAGTGATATCTATGCCATGGGGATTATTTTCTATGAGATGTTGACAGGCCATATCCCTTATGACG
GGGATAGCGCGGTGACCATTGCCCTCCAGCATTTCCAGAACCCCCTGCCGTCCGTTATTGCAGAAAATTCATCTGTACCT
CAGGCTTTAGAAAATGTTATTATCAAGGCAACTGCTAAAAAGTTGACCAATCGCTATCGCTCGGTTTCAGAGATGTATGT
AGACTTGTCTAGTAGCTTGTCCTACAATCGTAGAAATGAAAGTAAGTTAATCTTTGATGAAACGAGCAAGGCAGATACCA
AGACCTTGCCGAAGGTTTCTCAGAGTACCTTGACATCTATTCCTAAGGTTCAAGCGCAGACAGAACACAAATCAATCAAA
AACCCAAGCCAGGCTGTGACAGAGGAAACTTACCAACCACAAGCACCGAAAAAACATAGATTTAAGATGCGTTACCTGAT
TTTGTTGGCCAGCCTTGTATTGGTGGCAGCTTCTCTTATTTGGATACTATCCAGAACTCCTGCAACCATTGCCATTCCAG
ATGTGGCAGGTCAGACAGTTGCAGAGGCCAAGGCAACGCTCAAAAAAGCCAATTTTGAGATTGGTGAGGAGAAGACAGAG
GCTAGTGAAAAGGTGGAAGAAGGGCGGATTATCCGTACAGATCCTGGCGCTGGAACTGGTCGAAAAGAAGGAACGAAAAT
TAATCTGGTTGTCTCATCAGGCAAACAATCCTTCCAAATTAGTAATTATGTCGGCCGGAAATCTTCTGATGTTATCGCGG
AATTAAAAGAGAAAAAAGTTCCAGATAATTTGATTAAAATTGAGGAAGAAGAGTCGAATGAAAGTGAGGCTGGAACGGTC
CTGAAGCAAAGTCTACCAGAAGGTACGACCTATGACTTGAGCAAGGCAACTCAAATTGTTTTGACAGTAGCTAAAAAAGC
TACGACGATTCAATTAGGGAACTATATTGGACGGAACTCTACAGAAGTAATCTCAGAACTCAAGCAGAAGAAGGTTCCTG
AGAATTTGATTAAGATAGAGGAAGAAGAGTCCAGCGAAAGCGAACCAGGAACGATTATGAAACAAAGTCCAGGTGCCGGA
ACGACTTATGATGTGAGTAAACCTACTCAAATTGTCTTGACAGTAGCTAAAAAAGTTACAAGTGTTGCCATGCCGAGTTA
CATTGGTTCCAGCTTGGAGTTTACTAAGAACAATTTGATTCAAATTGTTGGGATTAAGGAAGCTAATATAGAAGTTGTAG
AAGTGACGACAGCGCCTGCAGGTAGTGTAGAAGGCATGGTTGTTGAACAAAGTCCTAGAGCAGGTGAAAAGGTAGACCTA
AATAAGACTAGAGTCAAGATTTCAATCTACAAACCTAAAACAACTTCAGCTACTCCTTAA

Domains

Predicted by InterproScan.

• Protein kinase domain (Pkinase) (12-243)

• PASTA domain (PASTA) (440-505)

• PASTA domain (PASTA) (513-577)

• PASTA domain (PASTA) (582-651)

• PASTA domain (PASTA) (371-432)

3D structure

• PDB: 5NOD

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
stkP	Streptococcus pneumoniae R6	100	100	1
stkP	Streptococcus pneumoniae Rx1	99.848	100	0.998
stkP	Streptococcus pneumoniae TIGR4	99.545	100	0.995
pknB	Streptococcus mutans UA159	52.217	98.864	0.516
stkP/pknB	Streptococcus salivarius strain HSISS4	59.109	79.167	0.468

References

• [1]: Germán E Piñas et al. (2018) Crosstalk between the serine/threonine kinase StkP and the response regulator ComE controls the stress response and intracellular survival of Streptococcus pneumoniae. PLoS Pathogens 14(6):e1007118. [PMID: 29883472]
• [2]: Makoto Osaki et al. (2009) The StkP/PhpP signaling couple in Streptococcus pneumoniae: cellular organization and physiological characterization. Journal of Bacteriology 191(15):4943-50. [PMID: 19502404]
• [3]: Lenka Sasková et al. (2007) Eukaryotic-type serine/threonine protein kinase StkP is a global regulator of gene expression in Streptococcus pneumoniae. Journal of Bacteriology 189(11):4168-79. [PMID: 17416671]
• [4]: Jose Echenique et al. (2004) Protein serine/threonine kinase StkP positively controls virulence and competence in Streptococcus pneumoniae. Infection And Immunity 72(4):2434-7. [PMID: 15039376]