Detailed information NTG0000384

Experimentally validated

Overview

• Name: pknB
• Type: Regulator
• Locus tag: SMU_RS02325
• Genome accession: NC_004350
• Coordinates: 452362..454212 (+)
• Length: 616 a.a.
• NCBI ID: WP_002263039.1
• Uniprot ID: A0AAX1K050
• Organism: Streptococcus mutans UA159
• Function: modulate the activity of ComDE; modulate the activity of VicKR Competence regulation

Function

A pknB mutant showed reduced expression of genes involved in bacteriocin production and genetic competence. PknB may modulate the activity of the two-component signal transduction systems VicKR and ComDE.

Genomic Context

Location: 447362..459212

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SMU_RS02310 (SMU.481)	fmt	449345..450280 (+)	936	WP_002263042.1	methionyl-tRNA formyltransferase	-
SMU_RS02315 (SMU.482)	rsmB	450261..451583 (+)	1323	WP_002291122.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
SMU_RS02320 (SMU.483)	-	451622..452365 (+)	744	WP_002263040.1	Stp1/IreP family PP2C-type Ser/Thr phosphatase	-
SMU_RS02325 (SMU.484)	pknB	452362..454212 (+)	1851	WP_002263039.1	Stk1 family PASTA domain-containing Ser/Thr kinase	Regulator
SMU_RS02330 (SMU.485)	liaF	454653..455348 (+)	696	WP_002263038.1	cell wall-active antibiotics response protein LiaF	-
SMU_RS02335 (SMU.486)	-	455345..456349 (+)	1005	WP_002263037.1	sensor histidine kinase	-
SMU_RS02340 (SMU.487)	-	456342..456983 (+)	642	WP_002263036.1	response regulator transcription factor	-
SMU_RS02345 (SMU.488)	-	457027..458442 (+)	1416	WP_002263035.1	Cof-type HAD-IIB family hydrolase	-
SMU_RS02350 (SMU.489)	-	458426..458812 (+)	387	WP_002263034.1	S1 RNA-binding domain-containing protein	-

Sequence

Protein

• Length: 616 a.a.
• Molecular weight: 66913.66 Da
• Isoelectric Point: 9.4520

>NTDB_id=384 SMU_RS02325 WP_002263039.1 452362..454212(+) (pknB) [Streptococcus mutans UA159]
MIQIGKLFAGRYRILQSIGRGGMADVYLANDLILDNEEVAIKVLRTNYQTDQVAVTRFQREARAMAELNHPNIVAIRDIG
EEDGQQFLAMEYVDGADLKKYIQDHAPLSNAEVIRIMKEVLSAMTLAHQKGIIHRDLKPQNVLLTKDGTAKVTDFGIAVA
FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIMLFEMLTGHIPYDGDSAVTIALQHFQKPLPSIIAENKNVP
QALENVVIKATAKRLTDRYHSTQEMLQDLRTSLQPNRSRERKLVFSDASDTKPLPKLEQAAANSLAAQSLKNKTSNQDKV
DHKSKPKTKPQPKPKKKRHFLARFFKIFFILVAIGIAVLTYLVLTKPSTVSVPDVSGDKLSTAKMIIRKSGLKVGDVQKI
EDDNVGAGKVVRTNPSAGSKKREGSSVDIFVASAKGFKMEDYTGQDYKDAIDNLTNNYGVSRDSIVLKEVSSDDYSGGTV
IGQSPKPGKTYHPSSDKKITLKVVKVTMPNLKNSTYEEAVSTLTAMGISSSRIKAYDASDYSSEISSPSSSSLVVGQSPY
YGNTVSLSSNDDIILYVSTSGGSHSGSSSSESSNSEGTTSSEASTDSSSSATTTSH

Nucleotide

• Length: 1851 bp

>NTDB_id=384 SMU_RS02325 WP_002263039.1 452362..454212(+) (pknB) [Streptococcus mutans UA159]
ATGATTCAGATTGGCAAATTATTTGCTGGTCGTTATCGGATTCTCCAGTCTATCGGACGAGGTGGGATGGCGGATGTTTA
TTTAGCTAATGATTTGATTTTAGATAATGAGGAAGTGGCCATTAAGGTTCTACGTACGAATTATCAGACAGATCAGGTTG
CTGTGACACGTTTTCAGCGGGAAGCACGTGCCATGGCAGAACTTAATCATCCTAATATTGTAGCCATTCGTGACATTGGA
GAAGAAGACGGTCAGCAATTCCTTGCAATGGAATATGTTGATGGCGCCGACTTGAAGAAATACATTCAAGATCACGCTCC
TTTATCAAATGCTGAAGTCATTAGAATCATGAAAGAGGTTCTTTCTGCTATGACTCTTGCCCACCAAAAGGGCATTATTC
ATCGGGATTTAAAACCTCAAAATGTTTTACTGACCAAAGATGGAACGGCTAAAGTAACAGACTTTGGTATTGCTGTGGCT
TTTGCTGAAACGAGTTTGACCCAAACCAATTCAATGCTGGGTTCAGTACATTACTTATCTCCTGAACAGGCGCGTGGTTC
TAAGGCTACTGTTCAAAGTGATATCTATGCAATGGGGATTATGCTTTTTGAAATGCTGACAGGGCATATTCCCTATGATG
GCGATAGTGCTGTTACAATTGCTTTGCAGCATTTTCAAAAGCCTTTACCATCTATTATTGCTGAGAATAAAAATGTCCCT
CAAGCTCTTGAAAATGTTGTTATTAAAGCAACAGCTAAACGATTAACGGACCGCTATCATTCAACGCAGGAGATGTTGCA
GGATTTAAGAACGTCTCTGCAGCCGAATCGCAGCCGTGAAAGAAAGCTTGTTTTCAGTGATGCTTCAGATACAAAACCAC
TACCAAAACTGGAACAGGCAGCAGCAAACTCTTTAGCTGCTCAGTCCTTAAAAAACAAAACTTCCAATCAGGATAAAGTA
GATCATAAATCTAAGCCTAAAACCAAACCACAACCTAAGCCTAAGAAAAAAAGACATTTTTTAGCTCGTTTCTTTAAAAT
CTTCTTTATATTAGTTGCCATTGGTATTGCTGTTTTGACCTACCTGGTTCTGACAAAACCGAGTACTGTTTCAGTTCCTG
ACGTTTCAGGAGATAAATTATCAACGGCCAAGATGATTATTAGAAAATCGGGTCTTAAGGTGGGTGACGTTCAAAAAATT
GAGGATGATAACGTTGGTGCTGGAAAGGTCGTAAGAACTAATCCAAGTGCAGGCAGTAAAAAACGTGAAGGTTCTAGTGT
TGATATTTTTGTAGCTAGTGCTAAGGGATTTAAGATGGAGGACTATACCGGTCAAGATTACAAGGATGCTATTGATAATC
TGACTAATAATTATGGTGTTTCTAGGGACAGTATTGTTCTTAAGGAAGTTTCATCTGATGACTACAGTGGTGGAACTGTT
ATCGGTCAATCACCAAAACCCGGAAAGACCTATCATCCTAGCAGTGATAAAAAAATAACTCTCAAGGTTGTCAAAGTCAC
TATGCCAAATCTTAAAAATTCTACTTATGAAGAGGCTGTCAGTACTTTAACAGCTATGGGGATTTCTTCAAGCCGTATAA
AAGCTTATGATGCCAGCGATTACTCATCAGAAATTTCATCACCATCATCTTCGTCACTCGTTGTCGGTCAAAGTCCTTAT
TATGGTAATACTGTCAGTCTGTCATCTAATGATGATATTATCTTGTATGTTTCAACATCAGGTGGCTCTCATTCTGGTTC
GTCGTCGTCTGAATCTTCGAACAGTGAAGGAACAACATCAAGCGAAGCCTCGACAGACTCTAGTTCAAGTGCGACGACAA
CTTCACATTAA

Domains

Predicted by InterproScan.

• Protein kinase domain (Pkinase) (12-258)

• PASTA domain (PASTA) (370-432)

• PASTA domain (PASTA) (506-579)

• PASTA domain (PASTA) (438-505)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
stkP/pknB	Streptococcus salivarius strain HSISS4	62.919	96.753	0.609
stkP	Streptococcus pneumoniae TIGR4	52.381	98.864	0.518
stkP	Streptococcus pneumoniae D39	52.217	98.864	0.516
stkP	Streptococcus pneumoniae R6	52.217	98.864	0.516
stkP	Streptococcus pneumoniae Rx1	52.053	98.864	0.515

References

• [1]: Michael Reck et al. (2016) Carolacton Treatment Causes Delocalization of the Cell Division Proteins PknB and DivIVa in Streptococcus mutans in vivo. Frontiers in Microbiology 7:684. [PMID: 27242711]
• [2]: Michael Reck et al. (2011) The biofilm inhibitor carolacton disturbs membrane integrity and cell division of Streptococcus mutans through the serine/threonine protein kinase PknB. Journal of Bacteriology 193(20):5692-706. [PMID: 21840978]
• [3]: Liliana Danusia Banu et al. (2010) The Streptococcus mutans serine/threonine kinase, PknB, regulates competence development, bacteriocin production, and cell wall metabolism. Infection And Immunity 78(5):2209-20. [PMID: 20231406]
• [4]: Haitham Hussain et al. (2006) A eukaryotic-type serine/threonine protein kinase is required for biofilm formation, genetic competence, and acid resistance in Streptococcus mutans. Journal of Bacteriology 188(4):1628-32. [PMID: 16452447]