Detailed information    

experimental Experimentally validated

Overview

Name   stkP   Type   Regulator
Locus tag   SP_RS08570 Genome accession   NC_003028
Coordinates   1634699..1636678 (-) Length   659 a.a.
NCBI ID   WP_000614538.1    Uniprot ID   A0A0B7L145
Organism   Streptococcus pneumoniae TIGR4     
Function   require for competence development; phosphorylate ComE   
Competence regulation

Function

StkP is involved in the regulation of cell wall synthesis and cell division/elongation in S. pneumoniae. A stkP mutant displays a strongly reduced natural transformability compared to wild type. StkP could phosphorylate ComE at Thr128 and the StkP/ComE pathway induced global changes in response to acidic stress.


Genomic Context

Location: 1629699..1641678
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SP_RS08540 (SP_1726) - 1629797..1631071 (-) 1275 WP_000704390.1 hydroxymethylglutaryl-CoA reductase, degradative -
  SP_RS08545 (SP_1727) - 1631071..1632243 (-) 1173 WP_000151960.1 hydroxymethylglutaryl-CoA synthase -
  SP_RS08550 (SP_1728) - 1632374..1632622 (-) 249 WP_001818278.1 hypothetical protein -
  SP_RS12355 (SP_1729) - 1632690..1633313 (-) 624 Protein_1630 transposase -
  SP_RS08560 (SP_1730) - 1633375..1633824 (-) 450 WP_000176924.1 threonine/serine exporter family protein -
  SP_RS08565 (SP_1731) - 1633826..1634584 (-) 759 WP_000392877.1 threonine/serine exporter family protein -
  SP_RS08570 (SP_1732) stkP 1634699..1636678 (-) 1980 WP_000614538.1 serine/threonine-protein kinase StkP Regulator
  SP_RS08575 (SP_1733) - 1636675..1637415 (-) 741 WP_000406247.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -
  SP_RS08580 (SP_1734) rsmB 1637430..1638743 (-) 1314 WP_001814363.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  SP_RS08585 (SP_1735) fmt 1638736..1639671 (-) 936 WP_000163693.1 methionyl-tRNA formyltransferase -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  stkP comE positive effect
  comE comE positive effect
  comE comX/comX1 positive effect
  comE comW positive effect
  comE comA positive effect
  comE comB positive effect
  comE comM positive effect
  comE comX/comX2 positive effect
  comE comD/comD2 positive effect
  comE comC/comC2 positive effect
  comD/comD2 comE positive effect
  comX/comX1 late competence genes positive effect
  clpP comX/comX1 negative effect
  comW comX/comX1 positive effect
  clpE comX/comX1 negative effect
  comW comX/comX2 positive effect
  clpP comW negative effect
  mecA comW negative effect
  clpC comW negative effect
  comA comC/comC2 positive effect
  comB comC/comC2 positive effect
  comM cbpD negative effect
  comX/comX2 late competence genes positive effect
  clpP comX/comX2 negative effect
  clpE comX/comX2 negative effect
  comC/comC2 comD/comD2 positive effect
  ciaH comC/comC2 negative effect
  htrA comC/comC2 negative effect
  ciaR comC/comC2 negative effect
  comX/comX2 late competence genes positive effect
  comX/comX1 late competence genes positive effect
  cbpD lytA positive effect
  cbpD lytC positive effect
  ciaH htrA positive effect
  htrA comEC/celB negative effect
  htrA comEA/celA/cilE negative effect
  ciaR htrA positive effect

Sequence

Protein


Download         Length: 659 a.a.        Molecular weight: 72288.81 Da        Isoelectric Point: 9.0945

>NTDB_id=146 SP_RS08570 WP_000614538.1 1634699..1636678(-) (stkP) [Streptococcus pneumoniae TIGR4]
MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVLRTNYQTDPIAVARFQREARAMADLDHPHIVRITDIG
EEDGQQYLAMEYVAGLDLKRYIKEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVA
FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAVTIALQHFQKPLPSVIAENPSVP
QALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYNRRNESKLIFDETSKADTKTLPKVSQSTLTSIPKVQAQTEHKSIK
NPSQAVTEETYQPQAPKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANFEIGEEKTE
ASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSDVIAELKEKKVPDNLIKIEEEESNESEAGTV
LKQSLPEGTTYDLSKATQIVLTVAKKATTIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAG
TTYDVSKPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSAEGMVVEQSPRAGEKVDL
NKTRVKISIYKPKTTSATP

Nucleotide


Download         Length: 1980 bp        

>NTDB_id=146 SP_RS08570 WP_000614538.1 1634699..1636678(-) (stkP) [Streptococcus pneumoniae TIGR4]
ATGATCCAAATCGGCAAGATTTTTGCCGGACGCTATCGGATTGTCAAACAGATTGGTCGAGGAGGTATGGCGGATGTCTA
CCTAGCCAAAGACTTAATCTTAGATGGGGAAGAAGTGGCAGTGAAGGTTCTGAGGACCAACTACCAGACGGACCCGATAG
CTGTAGCTCGTTTTCAGCGTGAAGCGAGAGCTATGGCAGATCTAGACCATCCTCATATCGTTCGGATAACAGATATTGGC
GAGGAAGACGGTCAACAGTACCTAGCTATGGAGTATGTGGCTGGACTGGACCTCAAACGCTATATCAAGGAACATTATCC
TCTTTCTAATGAAGAAGCAGTCCGTATCATGGGACAAATTCTCTTGGCTATGCGCTTGGCCCATACTCGAGGAATTGTTC
ACAGGGACTTGAAACCTCAAAATATCCTCTTGACACCAGATGGGACTGCCAAGGTCACAGACTTTGGGATTGCTGTAGCC
TTTGCAGAGACAAGTCTGACCCAGACTAACTCGATGTTGGGCTCAGTTCATTACTTGTCACCAGAGCAGGCGCGTGGTTC
GAAGGCGACTGTGCAGAGTGATATCTATGCCATGGGGATTATTTTCTATGAGATGCTGACAGGCCATATCCCTTATGACG
GGGATAGCGCGGTGACCATTGCCCTCCAGCATTTCCAGAAACCCCTGCCGTCCGTTATTGCAGAAAATCCATCTGTACCT
CAGGCTTTAGAAAATGTTATTATCAAGGCAACTGCTAAAAAGTTGACCAATCGCTACCGCTCGGTTTCAGAGATGTATGT
GGACTTGTCTAGTAGCTTGTCCTACAATCGTAGAAATGAAAGTAAGTTAATCTTTGATGAAACGAGCAAGGCAGATACCA
AGACCTTGCCGAAGGTTTCTCAGAGTACCTTGACATCTATTCCTAAGGTTCAAGCGCAAACAGAACACAAATCAATCAAA
AACCCAAGCCAGGCTGTGACAGAGGAAACTTACCAACCACAAGCACCGAAAAAACATAGATTTAAGATGCGTTACCTGAT
TTTGTTGGCCAGCCTTGTATTGGTGGCAGCTTCTCTTATTTGGATACTATCCAGAACTCCTGCAACCATTGCCATTCCAG
ATGTGGCAGGTCAGACAGTTGCAGAGGCCAAGGCAACGCTCAAAAAAGCCAATTTTGAGATTGGTGAGGAGAAGACAGAG
GCTAGTGAAAAGGTGGAAGAAGGGCGGATTATCCGTACAGATCCTGGCGCTGGAACTGGTCGAAAAGAAGGAACGAAAAT
CAATTTGGTTGTCTCATCAGGCAAGCAATCTTTCCAAATTAGTAATTATGTCGGTCGGAAATCCTCTGATGTCATTGCGG
AATTAAAAGAGAAAAAAGTTCCAGATAATTTGATTAAAATTGAGGAAGAAGAGTCGAATGAGAGTGAGGCTGGAACGGTC
CTGAAGCAAAGTCTACCAGAAGGTACGACCTATGACTTGAGCAAGGCAACTCAAATTGTTTTGACAGTAGCTAAAAAAGC
TACGACGATTCAATTAGGGAACTATATTGGACGGAACTCTACAGAAGTAATCTCAGAACTCAAGCAGAAGAAGGTTCCTG
AGAATTTGATTAAGATAGAGGAAGAAGAGTCCAGCGAAAGCGAACCAGGAACGATTATGAAACAAAGTCCAGGTGCCGGA
ACGACTTATGATGTGAGTAAACCTACTCAAATTGTCTTGACAGTAGCTAAAAAAGTTACAAGTGTTGCCATGCCGAGTTA
CATTGGTTCTAGCTTGGAGTTTACTAAGAACAATTTGATTCAAATTGTTGGGATTAAGGAAGCTAATATAGAAGTTGTAG
AAGTGACGACAGCGCCTGCAGGTAGTGCAGAAGGCATGGTTGTTGAACAAAGTCCTAGAGCAGGTGAAAAGGTAGACCTC
AATAAGACTAGAGTCAAGATTTCAATCTACAAACCTAAAACAACTTCAGCTACTCCTTAA

Domains

Predicted by InterproScan.

Protein kinase domain (Pkinase)

(12-227)

PASTA domain (PASTA)

(440-505)

PASTA domain (PASTA)

(582-651)

PASTA domain (PASTA)

(513-577)

PASTA domain (PASTA)

(371-432)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0B7L145

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  stkP Streptococcus pneumoniae D39

99.545

100

0.995
  stkP Streptococcus pneumoniae R6

99.545

100

0.995
  stkP Streptococcus pneumoniae Rx1

99.393

100

0.994
  pknB Streptococcus mutans UA159

52.381

98.864

0.518
  stkP/pknB Streptococcus salivarius strain HSISS4

59.312

79.167

0.47

Multiple sequence alignment    



References

[1] Germán E Piñas et al. (2018) Crosstalk between the serine/threonine kinase StkP and the response regulator ComE controls the stress response and intracellular survival of Streptococcus pneumoniae. PLoS Pathogens 14(6):e1007118. [PMID: 29883472]
[2] Makoto Osaki et al. (2009) The StkP/PhpP signaling couple in Streptococcus pneumoniae: cellular organization and physiological characterization. Journal of Bacteriology 191(15):4943-50. [PMID: 19502404]
[3] Lenka Sasková et al. (2007) Eukaryotic-type serine/threonine protein kinase StkP is a global regulator of gene expression in Streptococcus pneumoniae. Journal of Bacteriology 189(11):4168-79. [PMID: 17416671]
[4] Jose Echenique et al. (2004) Protein serine/threonine kinase StkP positively controls virulence and competence in Streptococcus pneumoniae. Infection And Immunity 72(4):2434-7. [PMID: 15039376]