Detailed information NTG0000467

Experimentally validated

Overview

• Name: stkP/pknB
• Type: Regulator
• Locus tag: HSISS4_RS06915
• Genome accession: NZ_CP013216
• Coordinates: 1542865..1544739 (-)
• Length: 624 a.a.
• NCBI ID: WP_021143821.1
• Uniprot ID: -
• Organism: Streptococcus salivarius strain HSISS4
• Function: activate comX expression Competence regulation

Function

Monitoring activation of those two promoters with or without stkP inhibition suggested that StkP level influences comX expression but has no impact on comR transcription.

Genomic Context

Location: 1537865..1549739

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HSISS4_RS06880 (HSISS4_01343)	rimM	1538243..1538761 (-)	519	WP_002884411.1	ribosome maturation factor RimM	-
HSISS4_RS06895 (HSISS4_01344)	-	1539457..1540101 (-)	645	WP_002884791.1	response regulator transcription factor	-
HSISS4_RS06900 (HSISS4_01345)	-	1540091..1541101 (-)	1011	WP_013990239.1	sensor histidine kinase	-
HSISS4_RS06905 (HSISS4_01346)	liaF	1541098..1541796 (-)	699	WP_014634809.1	cell wall-active antibiotics response protein LiaF	-
HSISS4_RS06910 (HSISS4_01347)	-	1541959..1542717 (-)	759	WP_014634810.1	potassium channel family protein	-
HSISS4_RS06915 (HSISS4_01348)	stkP/pknB	1542865..1544739 (-)	1875	WP_021143821.1	Stk1 family PASTA domain-containing Ser/Thr kinase	Regulator
HSISS4_RS06920 (HSISS4_01349)	-	1544739..1545476 (-)	738	WP_037620217.1	Stp1/IreP family PP2C-type Ser/Thr phosphatase	-
HSISS4_RS06925 (HSISS4_01350)	rsmB	1545520..1546842 (-)	1323	WP_059749268.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
HSISS4_RS06930 (HSISS4_01351)	fmt	1546832..1547767 (-)	936	WP_014634814.1	methionyl-tRNA formyltransferase	-

Regulatory network

Positive effect      

Negative effect

Regulator	Target	Regulation
stkP/pknB	comX/sigX	positive effect
comX/sigX	late competence genes	positive effect
comX/sigX	late competence genes	positive effect
comR	comX/sigX	positive effect
covS	comR	negative effect
covR	comR	negative effect
comS	comX/sigX	positive effect
pptA	comS	positive effect
amiD	comS	positive effect
pptB	comS	positive effect
amiE	comS	positive effect
amiC	comS	positive effect
amiF	comS	positive effect
pepF	comS	negative effect
amiA	comS	positive effect

Sequence

Protein

• Length: 624 a.a.
• Molecular weight: 67894.68 Da
• Isoelectric Point: 6.5789

>NTDB_id=467 HSISS4_RS06915 WP_021143821.1 1542865..1544739(-) (stkP/pknB) [Streptococcus salivarius strain HSISS4]
MIQVGKLFAGRYRILKSIGRGGMADVYLANDLILDNEEVAIKVLRTNYQTDQVAVARFQREARAMAELSHPNIVAIRDIG
EEDGQQFLVMEYVDGSDLKKYIQDHAPLSNQDVVRIMGEVLSAMTLAHQKGIIHRDLKPQNVLLTKDGTAKVTDFGIAVA
FAETSLTQTNSMLGSVHYLSPEQARGSKATIQSDIYAMGIMLFEMLTGHIPYDGDSAVTIALQHFQKPLPSILAENRNVP
QALENVVIRATAKKLENRYNSTLEMSRDLVTSLHPSHRRDAKVVFDDMTDTKTLPKVDPVPSASLEKKAVAAEPSEPTPA
PSKQPRKKTTPAKKKKKKNFFSTLLKVFLGLVFIGIIIFAYLVFTNPDNVQVPNVVGQELSTAQTKIEGAGFKVGEVKEV
EDDSVDTGKVIKTDPTAGTTRKEGSSIDVYVSSGSKGFALKDYKGKNYKDAIEDLTSNFGVSEDQIDIQHVEDDSAEEGE
ILSQSPGKNKSFNPKDSKAKIKFRVATPKIVTMPDVTGLTVSTAVQTLNRKNISSSSIEYHDYNTGAKLDKAKIPSSTEI
LYQDPQAGTSVDGTVILYVSVATASSSLQSSSSSTTHSSSTSSSTDSTTSSTETSTEATHTELQ

Nucleotide

• Length: 1875 bp

>NTDB_id=467 HSISS4_RS06915 WP_021143821.1 1542865..1544739(-) (stkP/pknB) [Streptococcus salivarius strain HSISS4]
ATGATCCAAGTTGGCAAATTGTTTGCTGGACGTTATCGAATCCTTAAATCTATCGGACGTGGTGGTATGGCGGATGTTTA
TCTGGCTAATGACCTGATTCTGGATAATGAAGAAGTGGCCATTAAGGTGCTTCGTACCAATTACCAAACGGATCAGGTGG
CTGTGGCGCGTTTCCAGCGTGAAGCGCGTGCCATGGCAGAACTCAGTCATCCTAATATCGTTGCTATCCGAGATATTGGC
GAAGAAGACGGCCAGCAGTTCTTGGTGATGGAGTATGTGGACGGTTCGGACTTGAAGAAATACATTCAAGACCATGCGCC
ACTTTCTAACCAAGATGTTGTGCGTATCATGGGGGAGGTACTTTCCGCCATGACACTTGCCCATCAAAAGGGCATTATCC
ACCGTGACCTTAAGCCACAAAACGTTCTTTTAACTAAGGACGGTACGGCCAAGGTTACAGACTTCGGAATTGCGGTTGCC
TTTGCAGAGACTAGCCTAACTCAAACCAACTCTATGTTGGGTTCTGTCCACTACCTCTCACCAGAGCAGGCTCGTGGATC
TAAAGCGACTATCCAGAGCGACATCTATGCTATGGGTATTATGCTTTTTGAAATGTTGACAGGTCATATTCCTTATGATG
GTGATAGTGCAGTAACCATTGCACTCCAACATTTCCAAAAGCCACTCCCATCGATTTTGGCAGAGAACCGTAACGTTCCT
CAAGCCTTGGAAAACGTGGTAATTCGAGCAACAGCCAAAAAACTAGAAAATCGTTATAATAGTACTTTGGAAATGAGCCG
TGACTTGGTAACGAGTTTGCATCCTAGTCATCGACGCGATGCCAAGGTAGTCTTTGATGATATGACGGATACTAAGACCT
TGCCAAAGGTTGATCCAGTTCCATCAGCAAGCCTTGAGAAAAAGGCTGTTGCTGCTGAGCCATCAGAGCCTACACCTGCT
CCAAGTAAACAACCAAGGAAAAAAACGACACCAGCTAAGAAGAAAAAGAAGAAGAATTTCTTCTCAACCTTGCTCAAGGT
TTTCCTAGGACTTGTCTTTATTGGTATCATCATCTTTGCCTATTTGGTATTTACCAATCCTGATAATGTCCAGGTGCCTA
ATGTGGTCGGTCAAGAGTTGTCCACAGCTCAGACTAAAATTGAGGGTGCTGGATTTAAGGTAGGAGAAGTCAAAGAAGTG
GAAGATGATTCTGTCGATACGGGCAAGGTCATCAAGACTGATCCTACAGCTGGAACTACACGAAAAGAAGGATCAAGCAT
TGATGTCTATGTTTCTTCTGGTAGTAAAGGTTTCGCTTTGAAAGACTACAAGGGTAAAAACTATAAGGATGCCATTGAGG
ACTTAACTTCGAATTTTGGTGTTTCTGAGGACCAAATTGATATTCAGCATGTCGAAGACGACAGTGCTGAAGAGGGAGAA
ATCCTATCTCAAAGTCCAGGGAAAAACAAGTCTTTCAATCCTAAGGATAGTAAGGCTAAGATTAAGTTCCGTGTGGCAAC
TCCTAAGATAGTTACCATGCCTGATGTGACTGGTTTGACGGTTTCAACAGCAGTTCAAACCTTGAATCGTAAGAATATCT
CAAGTAGTAGCATCGAGTACCACGACTATAATACTGGTGCTAAGTTAGATAAGGCTAAGATTCCTAGCAGTACGGAGATC
CTTTATCAAGATCCACAAGCTGGTACAAGTGTTGATGGTACAGTGATTCTCTATGTTTCGGTTGCAACAGCAAGTTCAAG
CCTACAGTCAAGCTCAAGCAGTACGACACATTCGTCATCGACGAGCTCAAGTACTGACAGTACAACTTCAAGCACTGAAA
CCTCGACAGAGGCTACTCACACAGAGCTACAGTAG

Domains

Predicted by InterproScan.

• PASTA domain (PASTA) (441-505)

• PASTA domain (PASTA) (370-432)

• PASTA domain (PASTA) (511-580)

• Protein kinase domain (Pkinase) (12-235)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pknB	Streptococcus mutans UA159	62.919	96.753	0.609
stkP	Streptococcus pneumoniae TIGR4	59.312	79.167	0.47
stkP	Streptococcus pneumoniae R6	59.109	79.167	0.468
stkP	Streptococcus pneumoniae D39	59.109	79.167	0.468
stkP	Streptococcus pneumoniae Rx1	58.907	79.167	0.466

References

• [1]: Adrien Knoops et al. (2022) A Genome-Wide CRISPR Interference Screen Reveals an StkP-Mediated Connection between Cell Wall Integrity and Competence in Streptococcus salivarius. MSystems 7(6):e0073522. [PMID: 36342134]