Detailed information    

experimental Experimentally validated

Overview

Name   comR   Type   Regulator
Locus tag   HSISS4_RS01285 Genome accession   NZ_CP013216
Coordinates   248145..249044 (+) Length   299 a.a.
NCBI ID   WP_021143546.1    Uniprot ID   -
Organism   Streptococcus salivarius strain HSISS4     
Function   activate transcription of comX   
Competence regulation

Function

The competence signaling system ComRS directly couples bacteriocin production and competence commitment. Stronger inhibitions of stkP were recorded for lower ComR levels, suggesting that ComR overproduction interferes with the StkP-mediated regulation of comX.


Genomic Context

Location: 243145..254044
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HSISS4_RS01265 (HSISS4_00213) trkA 244310..245659 (-) 1350 WP_021143542.1 Trk system potassium transporter TrkA -
  HSISS4_RS01270 (HSISS4_00214) - 245937..246446 (+) 510 WP_021143543.1 tRNA (cytidine(34)-2'-O)-methyltransferase -
  HSISS4_RS01275 (HSISS4_00215) - 246757..247314 (+) 558 WP_021143544.1 ECF transporter S component -
  HSISS4_RS01280 (HSISS4_00216) - 247317..247967 (+) 651 WP_002889918.1 phosphatase PAP2 family protein -
  HSISS4_RS01285 (HSISS4_00217) comR 248145..249044 (+) 900 WP_021143546.1 helix-turn-helix transcriptional regulator Regulator
  - comS 249132..249206 (+) 75 - - Regulator
  HSISS4_RS01290 (HSISS4_00218) comA 249219..251315 (+) 2097 WP_021143547.1 peptide cleavage/export ABC transporter Regulator
  HSISS4_RS01295 (HSISS4_00219) - 251426..251758 (+) 333 WP_002886546.1 DUF805 domain-containing protein -
  HSISS4_RS01300 (HSISS4_00220) - 251809..253200 (-) 1392 WP_021143548.1 DUF4153 domain-containing protein -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  comR comX/sigX positive effect
  comX/sigX late competence genes positive effect
  comX/sigX late competence genes positive effect
  stkP/pknB comX/sigX positive effect
  covS comR negative effect
  covR comR negative effect
  comS comX/sigX positive effect
  pptA comS positive effect
  amiD comS positive effect
  pptB comS positive effect
  amiE comS positive effect
  amiC comS positive effect
  amiF comS positive effect
  pepF comS negative effect
  amiA comS positive effect

Sequence

Protein


Download         Length: 299 a.a.        Molecular weight: 35184.65 Da        Isoelectric Point: 5.5756

>NTDB_id=469 HSISS4_RS01285 WP_021143546.1 248145..249044(+) (comR) [Streptococcus salivarius strain HSISS4]
MNIKDSIGLRIKTERERQQMSREVLCLDGAELTVRQLIRIEKGESLPSLDKLSYIAKRLGKNMTDLLDHDNITIPDEYYE
MKNRLIKFPTYRNPERIKAKLALIEEVYEKFFELLPEEELLTLDILENILSFTSWEESPKVEEIYEDLFEQVKRKRKFST
NDLLVIDYYFFHLYGRKQYDKKLFERIVKRVLNQEIWTDDVYNIVLFNDLMAIAALKIFHNSFSDFLTVVDKALAVIEKS
QFYSYKPSVFVLKAKYELLHKGNKKEAAENYNKAIMFASVLEDSVLEESIKAGKAADGL

Nucleotide


Download         Length: 900 bp        

>NTDB_id=469 HSISS4_RS01285 WP_021143546.1 248145..249044(+) (comR) [Streptococcus salivarius strain HSISS4]
TTGAACATAAAAGACAGCATTGGACTAAGAATCAAAACTGAGCGTGAACGCCAGCAGATGTCACGTGAAGTGCTATGTTT
AGATGGTGCGGAATTGACTGTTCGTCAGTTAATTCGCATTGAAAAAGGGGAGTCGCTCCCGTCTTTGGATAAATTATCTT
ATATTGCAAAACGTCTAGGAAAAAATATGACCGATTTATTGGATCATGATAATATTACTATCCCTGACGAATACTACGAG
ATGAAGAATCGTCTGATTAAGTTTCCAACGTATAGAAATCCAGAAAGAATAAAGGCTAAACTTGCCTTAATTGAGGAGGT
CTATGAGAAATTCTTTGAACTTCTTCCAGAAGAAGAATTGTTAACTCTAGATATTTTGGAAAATATTTTGAGTTTTACTA
GTTGGGAAGAGAGTCCTAAAGTTGAGGAGATATATGAAGACTTGTTTGAACAAGTCAAAAGGAAGAGGAAATTCTCAACT
AACGATTTATTAGTTATTGACTACTATTTCTTCCATCTTTATGGAAGAAAACAGTATGATAAAAAACTTTTTGAAAGAAT
CGTAAAGAGAGTATTAAATCAGGAAATTTGGACAGATGATGTCTATAATATTGTTTTGTTTAATGATTTGATGGCTATCG
CTGCTTTGAAGATTTTTCACAATTCCTTCTCAGACTTCTTAACAGTTGTGGATAAAGCCTTAGCTGTTATAGAAAAATCA
CAATTCTATAGCTATAAACCTAGTGTTTTTGTTCTCAAAGCCAAATATGAACTTCTTCATAAAGGAAACAAGAAAGAGGC
TGCAGAAAATTACAATAAGGCCATAATGTTTGCTTCCGTTTTAGAAGACTCGGTGCTAGAAGAAAGTATAAAGGCAGGTA
AAGCGGCAGATGGTTTATAG

Domains

Predicted by InterproScan.

ComR tetratricopeptide (ComR_TPR)

(75-292)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comR Streptococcus salivarius SK126

97.993

100

0.98
  comR Streptococcus thermophilus LMG 18311

95.318

100

0.953
  comR Streptococcus thermophilus LMD-9

94.649

100

0.946
  comR/comR2 Streptococcus sobrinus strain NIDR 6715-7

42.475

100

0.425
  comR/comR1 Streptococcus sobrinus strain NIDR 6715-7

42.14

100

0.421

Multiple sequence alignment    



References

[1] Adrien Knoops et al. (2022) A Genome-Wide CRISPR Interference Screen Reveals an StkP-Mediated Connection between Cell Wall Integrity and Competence in Streptococcus salivarius. MSystems 7(6):e0073522. [PMID: 36342134]
[2] Johann Mignolet et al. (2018) Circuitry Rewiring Directly Couples Competence to Predation in the Gut Dweller Streptococcus salivarius. Cell Reports 22(7):1627-1638. [PMID: 29444418]