Detailed information    

experimental Experimentally validated

Overview

Name   comR   Type   Regulator
Locus tag   STRSA0001_RS01280 Genome accession   NZ_ACLO01000018
Coordinates   82770..83669 (-) Length   299 a.a.
NCBI ID   WP_002883753.1    Uniprot ID   -
Organism   Streptococcus salivarius SK126     
Function   activate transcription of comX   
Competence regulation

Function

Once reimported, XIP then interacts with the transcriptional activator ComR and stimulates the binding of the ComR-XIP complex as a dimer to the ComR-box located in the promoter of comX and comS, thereby promoting their transcription


Genomic Context

Location: 77770..88669
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  STRSA0001_RS01265 (STRSA0001_1787) - 78596..79990 (+) 1395 WP_227826957.1 DUF4153 domain-containing protein -
  STRSA0001_RS01270 (STRSA0001_1788) - 80042..80374 (-) 333 WP_002883800.1 DUF805 domain-containing protein -
  STRSA0001_RS01275 (STRSA0001_1789) comA 80499..82595 (-) 2097 WP_002883724.1 peptide cleavage/export ABC transporter Regulator
  - comS 82608..82682 (-) 75 - - Regulator
  STRSA0001_RS01280 (STRSA0001_1790) comR 82770..83669 (-) 900 WP_002883753.1 helix-turn-helix transcriptional regulator Regulator
  STRSA0001_RS01285 (STRSA0001_1791) - 83849..84499 (-) 651 WP_002883719.1 phosphatase PAP2 family protein -
  STRSA0001_RS01290 (STRSA0001_1792) - 84502..85059 (-) 558 WP_002883726.1 ECF transporter S component -
  STRSA0001_RS01295 (STRSA0001_1794) - 85349..85858 (-) 510 WP_002883735.1 tRNA (cytidine(34)-2'-O)-methyltransferase -
  STRSA0001_RS01300 (STRSA0001_1795) trkA 86149..87498 (+) 1350 WP_002883814.1 Trk system potassium transporter TrkA -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  comR comX positive effect
  comX late competence genes positive effect
  comX late competence genes positive effect
  comR comS positive effect
  comS comX positive effect
  comS comS positive effect

Sequence

Protein


Download         Length: 299 a.a.        Molecular weight: 35105.55 Da        Isoelectric Point: 5.2183

>NTDB_id=51 STRSA0001_RS01280 WP_002883753.1 82770..83669(-) (comR) [Streptococcus salivarius SK126]
MNIKDSIGLRIKTERELQQMSREVLCLDGAELTVRQLIRIEKGESLPSLDKLSYIAKRLGKSMTDLLDHDNITIPDEYYE
MKNRLIKFPTYRNPERIKAKLALIEEVYEKFFELLSEEELLTLDILENILSFTSWEESPKVEEIYEDLFEQVKRKKKFST
NDLLVIDYYFFHLYGRKQYDKKLFERIVKRVLNQEIWTDDVYNIVLFNDLMAIAALKIFHNSFSDFLTVVDKALAVIEKS
QFYSYKPSVFVLKAKYELLHKGNKKEAAENYDKAIMFASVLEDSVLEESIRAGKAADGL

Nucleotide


Download         Length: 900 bp        

>NTDB_id=51 STRSA0001_RS01280 WP_002883753.1 82770..83669(-) (comR) [Streptococcus salivarius SK126]
TTGAACATAAAAGACAGCATTGGACTAAGAATCAAAACTGAGCGTGAACTCCAGCAGATGTCACGTGAAGTACTATGTTT
AGATGGTGCGGAATTAACTGTTCGTCAGTTAATTCGTATTGAAAAGGGAGAATCTCTCCCGTCTTTGGATAAATTATCTT
ATATCGCCAAACGTTTAGGAAAAAGTATGACTGATTTATTGGATCATGACAATATTACTATCCCTGACGAATACTACGAG
ATGAAGAATCGTTTGATTAAGTTTCCAACGTATAGAAATCCAGAAAGAATAAAGGCTAAACTTGCCTTAATTGAGGAGGT
CTACGAGAAATTCTTTGAACTTCTCTCAGAAGAAGAATTGTTAACTCTAGATATTTTGGAAAATATTTTGAGTTTTACTA
GTTGGGAAGAGAGTCCAAAAGTTGAGGAGATATATGAAGACTTGTTTGAACAAGTCAAAAGGAAGAAGAAATTCTCAACT
AACGATTTATTAGTTATTGACTATTATTTCTTCCATCTTTATGGAAGAAAACAGTATGATAAAAAACTTTTTGAAAGAAT
CGTAAAGAGAGTATTAAATCAGGAAATTTGGACAGACGATGTTTATAATATTGTTTTATTTAATGATTTGATGGCTATCG
CTGCTTTGAAGATTTTTCATAATTCCTTCTCAGACTTCTTAACAGTTGTGGATAAAGCCTTAGCTGTTATAGAAAAATCA
CAATTCTATAGCTATAAACCTAGTGTTTTTGTTCTCAAAGCCAAATATGAACTTCTTCATAAAGGAAACAAGAAAGAGGC
TGCAGAAAATTATGATAAGGCCATAATGTTTGCTTCCGTTTTAGAAGACTCGGTTTTAGAGGAAAGTATAAGGGCAGGAA
AAGCGGCAGATGGTTTATAG

Domains

Predicted by InterproScan.

ComR tetratricopeptide (ComR_TPR)

(75-292)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comR Streptococcus salivarius strain HSISS4

97.993

100

0.98
  comR Streptococcus thermophilus LMG 18311

94.649

100

0.946
  comR Streptococcus thermophilus LMD-9

93.98

100

0.94
  comR/comR2 Streptococcus sobrinus strain NIDR 6715-7

42.14

100

0.421
  comR/comR1 Streptococcus sobrinus strain NIDR 6715-7

41.137

100

0.411

Multiple sequence alignment    



References

[1] Laetitia Fontaine et al. (2010) A novel pheromone quorum-sensing system controls the development of natural competence in Streptococcus thermophilus and Streptococcus salivarius. Journal of Bacteriology 192(5):1444-54. [PMID: 20023010]