Detailed information    

experimental Experimentally validated

Overview

Name   comR   Type   Regulator
Locus tag   STER_RS01535 Genome accession   NC_008532
Coordinates   272013..272912 (+) Length   299 a.a.
NCBI ID   WP_011680720.1    Uniprot ID   -
Organism   Streptococcus thermophilus LMD-9     
Function   activate transcription of comX; activate transcription of comS   
Competence regulation

Function

Once reimported, XIP then interacts with the transcriptional activator ComR and stimulates the binding of the ComR-XIP complex as a dimer to the ComR-box located in the promoter of comX and comS, thereby promoting their transcription


Genomic Context

Location: 267013..277912
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  STER_RS01515 (STER_0312) trkA 268150..269499 (-) 1350 WP_011225444.1 Trk system potassium transporter TrkA -
  STER_RS01520 (STER_0313) - 269758..270297 (+) 540 WP_372585740.1 tRNA (cytidine(34)-2'-O)-methyltransferase -
  STER_RS01525 (STER_0314) - 270608..271165 (+) 558 WP_011680718.1 ECF transporter S component -
  STER_RS01530 (STER_0315) - 271168..271818 (+) 651 WP_011680719.1 phosphatase PAP2 family protein -
  STER_RS01535 (STER_0316) comR 272013..272912 (+) 900 WP_011680720.1 helix-turn-helix domain-containing protein Regulator
  - comS 273000..273071 (+) 72 - - Regulator
  STER_RS11550 - 273150..273731 (+) 582 Protein_243 cysteine peptidase family C39 domain-containing protein -
  STER_RS11555 comA 273716..274006 (+) 291 WP_194238295.1 ABC transporter transmembrane domain-containing protein Regulator
  STER_RS11560 comA 274349..274558 (+) 210 WP_002946147.1 hypothetical protein Regulator
  STER_RS01555 (STER_0318) - 274613..275181 (+) 569 Protein_246 ATP-binding cassette domain-containing protein -
  STER_RS11565 (STER_0319) - 275412..275600 (+) 189 WP_224103239.1 DUF805 domain-containing protein -
  STER_RS11575 (STER_0320) - 276093..276395 (-) 303 WP_224103194.1 hypothetical protein -
  STER_RS11580 (STER_0321) - 276619..276990 (-) 372 WP_224103195.1 hypothetical protein -
  STER_RS01570 (STER_0322) - 277396..277911 (+) 516 WP_002949547.1 AmiS/UreI family transporter -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  comR comX positive effect
  comX late competence genes positive effect
  comX late competence genes positive effect
  comR comS positive effect
  comS comX positive effect
  comS comS positive effect
  amiD comS positive effect
  pptA comS positive effect
  amiE comS positive effect
  eeP comS positive effect
  amiA3 comS positive effect
  pptB comS positive effect
  amiF comS positive effect
  amiC comS positive effect
  clpP comX negative effect
  mecA comX negative effect
  clpC comX negative effect

Sequence

Protein


Download         Length: 299 a.a.        Molecular weight: 35271.71 Da        Isoelectric Point: 5.1073

>NTDB_id=48 STER_RS01535 WP_011680720.1 272013..272912(+) (comR) [Streptococcus thermophilus LMD-9]
MNLKDSIGLRIKTERERQQMSREVLCLDGAELTVRQLIRIEKGESLPSLDRLSYIAKRLGKSMTELLDQDNITIPDEYYE
MKNRLIKFPTYRNPDRIKSKLTLIEEVYEKFFDILPEEELLTLDILENILSFTSWEESPKVEEIYEDLFEQVKRKRKFST
NDLLVIDYYFFHLYGRKQYDKKLFERIIKRVLNQEIWTDDVYNIVLFNDLMAIAALKIFHNSFSDFLTVVDKALAVIEKS
QLYSYKPSVFVLKAKYELLHKENKKEAAENYDKAIVFASVLEDSVLEESIKAGKLADGL

Nucleotide


Download         Length: 900 bp        

>NTDB_id=48 STER_RS01535 WP_011680720.1 272013..272912(+) (comR) [Streptococcus thermophilus LMD-9]
TTGAACTTAAAAGACAGCATTGGACTAAGAATCAAAACTGAGCGTGAACGCCAACAGATGTCACGTGAAGTGCTATGTTT
AGATGGTGCGGAATTGACTGTTCGCCAGTTAATTCGTATTGAAAAGGGGGAGTCTCTCCCGTCTTTAGATAGATTATCGT
ATATTGCTAAACGTTTAGGAAAAAGTATGACAGAGTTATTGGATCAAGACAATATTACCATTCCTGACGAATATTATGAA
ATGAAGAATCGTTTGATTAAGTTTCCAACGTACAGAAACCCTGACAGAATAAAGTCTAAACTTACTTTGATTGAGGAAGT
CTATGAGAAATTTTTTGATATTCTTCCAGAAGAAGAATTATTAACTTTAGACATTCTCGAAAATATATTGAGTTTTACTA
GCTGGGAGGAGAGTCCAAAAGTTGAGGAGATATATGAAGACTTGTTTGAACAAGTCAAAAGGAAGAGGAAATTCTCAACT
AACGATTTATTAGTCATTGACTATTATTTCTTTCATCTTTATGGGAGAAAACAGTATGACAAAAAACTATTTGAAAGAAT
TATAAAGAGAGTATTAAATCAGGAAATTTGGACAGATGATGTTTACAATATTGTTTTATTTAATGATTTGATGGCTATTG
CTGCTTTAAAGATTTTTCACAATTCCTTCTCAGACTTCTTAACAGTTGTGGATAAAGCCTTAGCTGTCATAGAAAAATCA
CAATTATATAGCTACAAGCCTAGTGTTTTTGTACTTAAGGCTAAATATGAACTTCTGCATAAAGAAAACAAGAAAGAGGC
TGCAGAGAATTATGATAAGGCCATAGTGTTTGCTTCCGTTTTGGAAGACTCGGTTTTAGAGGAAAGTATAAAGGCAGGAA
AATTGGCAGATGGTTTATAG

Domains

Predicted by InterproScan.

Helix-turn-helix (HTH_3)

(11-66)

ComR tetratricopeptide (ComR_TPR)

(75-292)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comR Streptococcus thermophilus LMG 18311

99.331

100

0.993
  comR Streptococcus salivarius strain HSISS4

94.649

100

0.946
  comR Streptococcus salivarius SK126

93.98

100

0.94
  comR/comR2 Streptococcus sobrinus strain NIDR 6715-7

42.14

100

0.421
  comR/comR1 Streptococcus sobrinus strain NIDR 6715-7

41.472

100

0.415

Multiple sequence alignment    



References

[1] Laura Ledesma-García et al. (2021) Coevolution of the bacterial pheromone ComS and sensor ComR fine-tunes natural transformation in streptococci. The Journal of Biological Chemistry 297(6):101346. [PMID: 34715127]
[2] Laura Ledesma-Garcia et al. (2020) Molecular dissection of pheromone selectivity in the competence signaling system ComRS of streptococci. Proceedings of The National Academy of Sciences of The United States of America 117(14):7745-7754. [PMID: 32198205]
[3] Antoine Talagas et al. (2016) Structural Insights into Streptococcal Competence Regulation by the Cell-to-Cell Communication System ComRS. PLoS Pathogens 12(12):e1005980. [PMID: 27907189]
[4] Laurie Haustenne et al. (2015) Modeling of the ComRS Signaling Pathway Reveals the Limiting Factors Controlling Competence in Streptococcus thermophilus. Frontiers in Microbiology 6:1413. [PMID: 26733960]
[5] Laetitia Fontaine et al. (2013) Mechanism of competence activation by the ComRS signalling system in streptococci. Molecular Microbiology 87(6):1113-32. [PMID: 23323845]
[6] Rozenn Gardan et al. (2013) Extracellular life cycle of ComS, the competence-stimulating peptide of Streptococcus thermophilus. Journal of Bacteriology 195(8):1845-55. [PMID: 23396911]
[7] Laetitia Fontaine et al. (2010) A novel pheromone quorum-sensing system controls the development of natural competence in Streptococcus thermophilus and Streptococcus salivarius. Journal of Bacteriology 192(5):1444-54. [PMID: 20023010]