Detailed information    

experimental Experimentally validated

Overview

Name   comX   Type   Regulator
Locus tag   STER_RS00925 Genome accession   NC_008532
Coordinates   155516..156013 (+) Length   165 a.a.
NCBI ID   WP_002949220.1    Uniprot ID   Q5M6C4
Organism   Streptococcus thermophilus LMD-9     
Function   activate transcription of late competence genes   
Competence regulation

Function

ComX controls expression of the late competence genes, which are essential for DNA binding, uptake and recombination.


Genomic Context

Location: 150516..161013
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  STER_RS10840 - 151930..152252 (-) 323 Protein_119 transposase -
  STER_RS00910 (STER_0186) - 152441..152767 (+) 327 WP_014607875.1 M20/M25/M40 family metallo-hydrolase -
  STER_RS00915 (STER_0187) - 152849..154039 (+) 1191 WP_011680651.1 SAM-dependent methyltransferase -
  STER_RS00920 (STER_0188) - 154298..154762 (-) 465 Protein_122 transposase family protein -
  STER_RS09965 - 155120..155224 (+) 105 Protein_123 IS200/IS605 family transposase -
  STER_RS00925 (STER_0189) comX 155516..156013 (+) 498 WP_002949220.1 sigma factor Regulator
  STER_RS00930 (STER_0190) - 156113..156955 (-) 843 WP_023909339.1 mechanosensitive ion channel family protein -
  STER_RS00935 (STER_0191) tig 157096..158379 (+) 1284 WP_011680653.1 trigger factor -
  STER_RS00940 (STER_0192) rpoE 158537..159112 (+) 576 WP_002949228.1 DNA-directed RNA polymerase subunit delta -
  STER_RS00945 (STER_0193) - 159363..160967 (+) 1605 WP_002949230.1 CTP synthase -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  comX late competence genes positive effect
  comX late competence genes positive effect
  comR comX positive effect
  comS comX positive effect
  clpP comX negative effect
  mecA comX negative effect
  clpC comX negative effect
  comR comS positive effect
  comS comS positive effect
  amiD comS positive effect
  pptA comS positive effect
  amiE comS positive effect
  eeP comS positive effect
  amiA3 comS positive effect
  pptB comS positive effect
  amiF comS positive effect
  amiC comS positive effect

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 20160.12 Da        Isoelectric Point: 5.2073

>NTDB_id=47 STER_RS00925 WP_002949220.1 155516..156013(+) (comX) [Streptococcus thermophilus LMD-9]
MEQEVFVKAYEKVRPIVLKAFRQYFIQLWDQADMEQEAMMTLYQLLKKFPDLEKDDDKLRRYFKTKFRNRLNDEVRRQES
VKRQANRQCYVEISDIAFCIPNKELDMVDRLAYDEQLNAFREQLSSEDSLKLDRLLGGECFRGRKKMIRELRFWMVDFDP
CNEED

Nucleotide


Download         Length: 498 bp        

>NTDB_id=47 STER_RS00925 WP_002949220.1 155516..156013(+) (comX) [Streptococcus thermophilus LMD-9]
ATGGAACAAGAAGTTTTTGTTAAGGCATATGAAAAGGTAAGGCCAATTGTACTTAAGGCTTTTAGGCAATACTTTATTCA
GCTTTGGGATCAAGCTGACATGGAGCAAGAGGCGATGATGACTTTGTATCAGCTTTTAAAAAAGTTTCCTGATTTAGAGA
AAGATGATGATAAGTTACGTCGTTACTTTAAAACTAAGTTTAGGAATCGACTTAATGATGAAGTGAGGCGGCAGGAGTCA
GTAAAACGTCAAGCTAATAGACAGTGCTATGTTGAAATTTCAGATATTGCCTTTTGTATTCCCAATAAGGAGCTAGATAT
GGTTGATAGACTTGCTTATGATGAACAGCTTAATGCTTTTCGTGAGCAGTTATCATCGGAAGATTCTCTTAAGTTGGATC
GATTGTTGGGTGGTGAATGCTTTAGGGGAAGGAAAAAGATGATACGAGAGTTAAGATTTTGGATGGTTGACTTTGATCCA
TGTAATGAAGAAGACTGA

Domains

Predicted by InterProScan.

Sigma-70 region 2 (Sigma70_r2)

(10-79)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5M6C4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comX Streptococcus thermophilus LMG 18311

100

100

1
  comX/sigX Streptococcus salivarius strain HSISS4

89.091

100

0.891
  comX Streptococcus salivarius SK126

88.485

100

0.885
  comR/comR2 Streptococcus gordonii str. Challis substr. CH1

42.581

96.875

0.413
  comR/comR1 Streptococcus gordonii str. Challis substr. CH1

42.581

96.875

0.413
  comX Streptococcus sobrinus strain NIDR 6715-7

41.975

98.182

0.412
  comX/sigX Streptococcus infantarius subsp. infantarius ATCC BAA-102

41.667

98.734

0.411
  comX/sigX Streptococcus mutans UA159

41.026

97.5

0.4
  comX/sigX Streptococcus suis P1/7

40.523

98.077

0.397
  comX/sigX Streptococcus suis D9

40.523

98.077

0.397
  comX/sigX Streptococcus suis isolate S10

40.523

98.077

0.397
  comX/sigX/comX2/sigX2 Streptococcus pyogenes JRS4

42.667

90.909

0.388
  comX/sigX/comX1/sigX1 Streptococcus pyogenes JRS4

42.667

90.909

0.388
  comX/sigX/comX2/sigX2 Streptococcus pyogenes MGAS8232

42.667

90.909

0.388
  comX/sigX/comX1/sigX1 Streptococcus pyogenes MGAS8232

42.667

90.909

0.388
  comX/sigX/comX2/sigX2 Streptococcus pyogenes MGAS315

42

90.909

0.382
  comX/sigX/comX1/sigX1 Streptococcus pyogenes MGAS315

42

90.909

0.382
  comX/comX2 Streptococcus pneumoniae TIGR4

38.926

93.711

0.365
  comX/comX1 Streptococcus pneumoniae TIGR4

38.926

93.711

0.365
  comX/sigX/comX2/sigX2 Streptococcus mitis SK321

37.419

97.484

0.365
  comX/sigX/comX1/sigX1 Streptococcus mitis SK321

37.419

97.484

0.365

Multiple sequence alignment    



References

[1] Laetitia Fontaine et al. (2013) Mechanism of competence activation by the ComRS signalling system in streptococci. Molecular Microbiology 87(6):1113-32. [PMID: 23323845]
[2] Laetitia Fontaine et al. (2010) A novel pheromone quorum-sensing system controls the development of natural competence in Streptococcus thermophilus and Streptococcus salivarius. Journal of Bacteriology 192(5):1444-54. [PMID: 20023010]
[3] Trinelise Blomqvist et al. (2006) Natural genetic transformation: A novel tool for efficient genetic engineering of the dairy bacterium Streptococcus thermophilus. Applied And Environmental Microbiology 72(10):6751-6. [PMID: 17021227]