Detailed information    

experimental Experimentally validated

Overview

Name   comX/sigX/comX2/sigX2   Type   Regulator
Locus tag   SpyM6JRS4_RS07800 Genome accession   NZ_CP011414
Coordinates   1550924..1551475 (-) Length   183 a.a.
NCBI ID   WP_010921894.1    Uniprot ID   -
Organism   Streptococcus pyogenes JRS4     
Function   activate transcription of late competence genes   
Competence regulation

Function

Expression of sigX from this promoter led to transcription of the sigma(X)-dependent cinA promoter in GAS cells. We found that expression of the sigX gene in a clpP mutant strain resulted in greater accumulation of sigma(X) protein, which resulted in higher levels of transcription from the sigma(X)-dependent promoters cinA, smf, and cglA.


Genomic Context

Location: 1545924..1556475
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SpyM6JRS4_RS07775 (SpyM6JRS4_07770) - 1547292..1548137 (+) 846 WP_002982833.1 mechanosensitive ion channel family protein -
  SpyM6JRS4_RS07780 (SpyM6JRS4_07775) - 1548202..1548762 (-) 561 WP_002995157.1 TIGR01440 family protein -
  SpyM6JRS4_RS07785 (SpyM6JRS4_07780) - 1548776..1549246 (-) 471 WP_011184949.1 ECF transporter S component -
  SpyM6JRS4_RS07790 (SpyM6JRS4_07785) - 1549236..1550000 (-) 765 WP_002982821.1 bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase -
  SpyM6JRS4_RS07795 (SpyM6JRS4_07790) truA 1549990..1550739 (-) 750 WP_002988132.1 tRNA pseudouridine(38-40) synthase TruA -
  SpyM6JRS4_RS07800 (SpyM6JRS4_07795) comX/sigX/comX2/sigX2 1550924..1551475 (-) 552 WP_010921894.1 sigma-70 family RNA polymerase sigma factor Regulator

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  comX/sigX/comX2/sigX2 late competence genes positive effect
  comX/sigX/comX2/sigX2 late competence genes positive effect
  clpP comX/sigX/comX2/sigX2 negative effect
  comX/sigX/comX1/sigX1 late competence genes positive effect
  comX/sigX/comX1/sigX1 late competence genes positive effect
  clpP comX/sigX/comX1/sigX1 negative effect

Sequence

Protein


Download         Length: 183 a.a.        Molecular weight: 22281.08 Da        Isoelectric Point: 10.0375

>NTDB_id=448 SpyM6JRS4_RS07800 WP_010921894.1 1550924..1551475(-) (comX/sigX/comX2/sigX2) [Streptococcus pyogenes JRS4]
MSCCFFVVLFILGIVKKKRRLKMSIETRAAFEKVKPIILKLKRHYYIQLWDRDDWLQEGHIILLQLLERYPELIEEEERL
YRYFKTKFSSYLKDLLRRQESQKRQFHKLAYEEIGEVAHAIPSRGLWLDDYVAYQEVIASLENQLNSQERMQFQALIRGE
RFKGRRALLRKISPYFKEFAQQL

Nucleotide


Download         Length: 552 bp        

>NTDB_id=448 SpyM6JRS4_RS07800 WP_010921894.1 1550924..1551475(-) (comX/sigX/comX2/sigX2) [Streptococcus pyogenes JRS4]
ATGTCCTGTTGTTTTTTTGTGGTTCTCTTTATACTAGGGATAGTTAAAAAGAAAAGGAGACTCAAAATGTCGATAGAGAC
AAGAGCAGCGTTTGAAAAAGTTAAGCCCATTATTTTGAAATTAAAGCGACACTATTATATTCAATTGTGGGATAGAGATG
ACTGGTTACAAGAAGGACATATTATCTTATTACAGTTACTAGAAAGGTACCCAGAGTTAATTGAGGAAGAAGAGCGTTTA
TATCGCTATTTTAAAACAAAATTTTCATCTTATTTGAAAGATTTATTACGCCGTCAAGAAAGTCAAAAGCGTCAGTTCCA
TAAGTTAGCATATGAAGAGATAGGGGAGGTTGCACATGCCATTCCATCGAGAGGGTTATGGCTAGACGACTATGTGGCTT
ATCAAGAGGTAATAGCTAGCTTAGAGAACCAATTAAATTCGCAAGAGCGTATGCAGTTTCAAGCACTTATCAGGGGCGAA
CGTTTCAAGGGAAGACGTGCTCTACTTAGGAAGATCAGTCCCTATTTTAAGGAATTTGCACAGCAGTTGTAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comX/sigX/comX1/sigX1 Streptococcus pyogenes JRS4

100

100

1
  comX/sigX/comX2/sigX2 Streptococcus pyogenes MGAS8232

99.454

100

0.995
  comX/sigX/comX1/sigX1 Streptococcus pyogenes MGAS8232

99.454

100

0.995
  comX/sigX/comX2/sigX2 Streptococcus pyogenes MGAS315

99.454

100

0.995
  comX/sigX/comX1/sigX1 Streptococcus pyogenes MGAS315

99.454

100

0.995
  comX/sigX Streptococcus infantarius subsp. infantarius ATCC BAA-102

49.333

94.937

0.468
  comX/sigX Streptococcus mutans UA159

44.444

95.625

0.425
  comX/sigX/comX2/sigX2 Streptococcus mitis SK321

42.953

93.711

0.403
  comX/sigX Streptococcus suis P1/7

40

99.359

0.397
  comX/sigX Streptococcus suis D9

40

99.359

0.397
  comX/sigX Streptococcus suis isolate S10

40

99.359

0.397
  comX/sigX/comX2/sigX2 Streptococcus mitis NCTC 12261

42.282

93.711

0.396
  comX Streptococcus sobrinus strain NIDR 6715-7

46.104

84.153

0.388
  comX Streptococcus thermophilus LMD-9

42.667

90.909

0.388
  comX/sigX Streptococcus salivarius strain HSISS4

42.667

90.909

0.388
  comX Streptococcus thermophilus LMG 18311

42.667

90.909

0.388
  comX Streptococcus salivarius SK126

42.667

90.909

0.388
  comR/comR1 Streptococcus gordonii str. Challis substr. CH1

40.26

96.25

0.388
  comR/comR2 Streptococcus gordonii str. Challis substr. CH1

40.26

96.25

0.388
  comX/sigX/comX1/sigX1 Streptococcus mitis SK321

40.94

93.711

0.384
  comX/comX2 Streptococcus pneumoniae TIGR4

40

94.34

0.377
  comX/comX1 Streptococcus pneumoniae TIGR4

40

94.34

0.377
  comX/comX1 Streptococcus pneumoniae R6

40

94.34

0.377
  comX/comX1 Streptococcus pneumoniae D39

40

94.34

0.377
  comX/comX2 Streptococcus pneumoniae R6

40

94.34

0.377
  comX/comX2 Streptococcus pneumoniae Rx1

40

94.34

0.377
  comX/comX1 Streptococcus pneumoniae Rx1

40

94.34

0.377
  comX/comX2 Streptococcus pneumoniae D39

40

94.34

0.377
  comX/sigX/comX1/sigX1 Streptococcus mitis NCTC 12261

40.268

93.711

0.377

Multiple sequence alignment    



References

[1] Robyn L Woodbury et al. (2006) Sigma X induces competence gene expression in Streptococcus pyogenes. Research in Microbiology 157(9):851-6. [PMID: 16963231]
[2] Jason A Opdyke et al. (2003) Expression of the secondary sigma factor sigmaX in Streptococcus pyogenes is restricted at two levels. Journal of Bacteriology 185(15):4291-7. [PMID: 12867436]