Detailed information    

experimental Experimentally validated

Overview

Name   comX/sigX/comX2/sigX2   Type   Regulator
Locus tag   SM12261_RS01070 Genome accession   NZ_CP028414
Coordinates   213172..213651 (+) Length   159 a.a.
NCBI ID   WP_000471940.1    Uniprot ID   A0AAX2L348
Organism   Streptococcus mitis NCTC 12261     
Function   activate transcription of late competence genes   
Competence regulation

Function

Upregulated in response to CSP as measured by RNA-seq.


Genomic Context

Location: 208172..218651
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SM12261_RS01045 (SM12261_0203) - 208914..209789 (-) 876 WP_004238891.1 RluA family pseudouridine synthase -
  SM12261_RS01050 (SM12261_0204) pbp2a 209876..212071 (+) 2196 WP_000762651.1 penicillin-binding protein PBP2A -
  SM12261_RS01055 rpmG 212124..212276 (+) 153 WP_001809375.1 50S ribosomal protein L33 -
  SM12261_RS01060 (SM12261_0205) secE 212286..212462 (+) 177 WP_001210990.1 preprotein translocase subunit SecE -
  SM12261_RS01065 (SM12261_0206) nusG 212516..213052 (+) 537 WP_000376740.1 transcription termination/antitermination protein NusG -
  SM12261_RS01070 (SM12261_0207) comX/sigX/comX2/sigX2 213172..213651 (+) 480 WP_000471940.1 sigma-70 family RNA polymerase sigma factor Regulator

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  comX/sigX/comX2/sigX2 late competence genes positive effect
  comX/sigX/comX2/sigX2 late competence genes positive effect
  comE comX/sigX/comX2/sigX2 positive effect
  comW comX/sigX/comX2/sigX2 positive effect
  comX/sigX/comX1/sigX1 late competence genes positive effect
  comX/sigX/comX1/sigX1 late competence genes positive effect
  comW comX/sigX/comX1/sigX1 positive effect
  comE comX/sigX/comX1/sigX1 positive effect
  comE comD positive effect
  comD comE positive effect
  comC comD positive effect
  comE comE positive effect
  comE comB positive effect
  comE comC positive effect
  comE comM positive effect
  comE comW positive effect
  comE comA positive effect
  comB comC positive effect
  comA comC positive effect
  comM cbpD negative effect

Sequence

Protein


Download         Length: 159 a.a.        Molecular weight: 20103.81 Da        Isoelectric Point: 7.1188

>NTDB_id=489 SM12261_RS01070 WP_000471940.1 213172..213651(+) (comX/sigX/comX2/sigX2) [Streptococcus mitis NCTC 12261]
MFKELYEEVQGIVYKCRNEYYLHLWELSDWDQEGMICLHELISREEELVEDIPRLRKYFKTKFRNRILDHIRKQESQKRR
YDKEPYEEVGEISHRISEGGMWLDEYYLFHETLRDYRNKQSKDKQEELERVLRHERFRGRQRVLRDLRIVFKEFDIRTH

Nucleotide


Download         Length: 480 bp        

>NTDB_id=489 SM12261_RS01070 WP_000471940.1 213172..213651(+) (comX/sigX/comX2/sigX2) [Streptococcus mitis NCTC 12261]
ATGTTTAAAGAATTATATGAAGAAGTCCAAGGAATTGTATACAAGTGTAGAAATGAATATTACCTTCATTTATGGGAGCT
ATCAGATTGGGACCAAGAGGGAATGATTTGCTTACATGAATTGATCAGTAGAGAAGAAGAGCTAGTAGAAGATATTCCTC
GTTTACGTAAATACTTCAAAACTAAATTCCGTAATCGAATTTTAGACCATATCCGTAAACAAGAAAGCCAGAAGCGTAGA
TATGATAAAGAACCCTATGAAGAAGTAGGTGAGATTAGTCATCGTATCAGTGAGGGAGGTATGTGGCTAGATGAGTATTA
TCTCTTTCATGAGACACTAAGAGATTATAGAAACAAACAAAGTAAAGACAAACAAGAAGAGTTAGAACGCGTCTTAAGAC
ATGAACGCTTCCGAGGGCGTCAAAGAGTATTGAGAGACTTACGTATTGTGTTTAAGGAGTTTGATATCCGTACTCATTAG

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comX/sigX/comX1/sigX1 Streptococcus mitis NCTC 12261

97.484

100

0.975
  comX/sigX/comX2/sigX2 Streptococcus mitis SK321

94.34

100

0.943
  comX/sigX/comX1/sigX1 Streptococcus mitis SK321

94.304

99.371

0.937
  comX/comX1 Streptococcus pneumoniae Rx1

92.453

100

0.925
  comX/comX2 Streptococcus pneumoniae Rx1

92.453

100

0.925
  comX/comX1 Streptococcus pneumoniae R6

92.453

100

0.925
  comX/comX2 Streptococcus pneumoniae R6

92.453

100

0.925
  comX/comX2 Streptococcus pneumoniae D39

92.453

100

0.925
  comX/comX1 Streptococcus pneumoniae D39

92.453

100

0.925
  comX/comX2 Streptococcus pneumoniae TIGR4

91.824

100

0.918
  comX/comX1 Streptococcus pneumoniae TIGR4

91.824

100

0.918
  comR/comR2 Streptococcus gordonii str. Challis substr. CH1

49.359

98.113

0.484
  comR/comR1 Streptococcus gordonii str. Challis substr. CH1

49.359

98.113

0.484
  comX/sigX Streptococcus mutans UA159

47.712

96.226

0.459
  comX/sigX Streptococcus suis P1/7

45.752

98.077

0.449
  comX/sigX Streptococcus suis D9

45.752

98.077

0.449
  comX/sigX Streptococcus suis isolate S10

45.752

98.077

0.449
  comX/sigX/comX2/sigX2 Streptococcus pyogenes JRS4

42.282

93.711

0.396
  comX/sigX/comX2/sigX2 Streptococcus pyogenes MGAS8232

42.282

93.711

0.396
  comX/sigX/comX1/sigX1 Streptococcus pyogenes JRS4

42.282

93.711

0.396
  comX/sigX/comX1/sigX1 Streptococcus pyogenes MGAS8232

42.282

93.711

0.396
  comX/sigX/comX2/sigX2 Streptococcus pyogenes MGAS315

41.447

95.597

0.396
  comX/sigX/comX1/sigX1 Streptococcus pyogenes MGAS315

41.447

95.597

0.396
  comX Streptococcus sobrinus strain NIDR 6715-7

37.662

96.855

0.365
  comX Streptococcus salivarius SK126

37.419

97.484

0.365

Multiple sequence alignment    



References

[1] G Salvadori et al. (2018) High-resolution profiles of the Streptococcus mitis CSP signaling pathway reveal core and strain-specific regulated genes. BMC Genomics 19(1):453. [PMID: 29898666]