Detailed information NTG0000483

Experimentally validated

Overview

• Name: comA
• Type: Regulator
• Locus tag: SM12261_RS00275
• Genome accession: NZ_CP028414
• Coordinates: 54785..56938 (+)
• Length: 717 a.a.
• NCBI ID: WP_000668315.1
• Uniprot ID: -
• Organism: Streptococcus mitis NCTC 12261
• Function: processing and transport of ComC Competence regulation

Function

Upregulated in response to CSP as measured by RNA-seq.

Genomic Context

Location: 49785..61938

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SM12261_RS09560 (SM12261_0050)	-	51502..52317 (+)	816	WP_000184940.1	GHKL domain-containing protein	-
SM12261_RS00250 (SM12261_0051)	-	52314..53045 (+)	732	WP_001221869.1	response regulator transcription factor	-
SM12261_RS00255 (SM12261_0052)	-	53291..53419 (+)	129	WP_000658220.1	smi_0059.1 family bacteriocin-like peptide	-
SM12261_RS09435	-	53431..53586 (+)	156	WP_001004642.1	hypothetical protein	-
SM12261_RS00265 (SM12261_0053)	-	53620..53793 (+)	174	WP_000180829.1	class IIb bacteriocin, lactobin A/cerein 7B family	-
SM12261_RS00270 (SM12261_0054)	-	53917..54069 (+)	153	WP_001180497.1	class IIb bacteriocin, lactobin A/cerein 7B family	-
SM12261_RS09440 (SM12261_0055)	-	54094..54249 (+)	156	WP_000732248.1	hypothetical protein	-
SM12261_RS00275 (SM12261_0056)	comA	54785..56938 (+)	2154	WP_000668315.1	peptide cleavage/export ABC transporter ComA	Regulator
SM12261_RS00280 (SM12261_0057)	comB	56951..58300 (+)	1350	WP_000801588.1	competence pheromone export protein ComB	Regulator
SM12261_RS00285 (SM12261_0058)	purC	58470..59177 (+)	708	WP_000043282.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
SM12261_RS09700	-	59179..59322 (+)	144	WP_078228447.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-

Regulatory network

Positive effect      

Negative effect

Regulator	Target	Regulation
comA	comC	positive effect
comC	comD	positive effect
comD	comE	positive effect
comE	comX/sigX/comX2/sigX2	positive effect
comX/sigX/comX2/sigX2	late competence genes	positive effect
comX/sigX/comX2/sigX2	late competence genes	positive effect
comW	comX/sigX/comX2/sigX2	positive effect
comX/sigX/comX1/sigX1	late competence genes	positive effect
comX/sigX/comX1/sigX1	late competence genes	positive effect
comW	comX/sigX/comX1/sigX1	positive effect
comE	comX/sigX/comX1/sigX1	positive effect
comE	comD	positive effect
comE	comE	positive effect
comE	comB	positive effect
comE	comC	positive effect
comE	comM	positive effect
comE	comW	positive effect
comE	comA	positive effect
comB	comC	positive effect
comM	cbpD	negative effect

Sequence

Protein

• Length: 717 a.a.
• Molecular weight: 80194.19 Da
• Isoelectric Point: 6.2574

>NTDB_id=483 SM12261_RS00275 WP_000668315.1 54785..56938(+) (comA) [Streptococcus mitis NCTC 12261]
MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYSLAHLRELAKTTMDGTTALGLVKVAEEIGFETRAIKADMTLFDLPDL
TFPFVAHVLKEGKLLHYYVVTGQDKDSIHIADPDPGVKLTKLPRERFAEEWTGVTLFMAPSPDYKPHKDQKNGLLSFIPI
LVKQRGLIANIVLATLLVTLINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYILQQILSYAQEYLLLVLGQRLSID
VILSYIKHVFHLPMSFFATRRTGEIVSRFTDANSIIDALASTILSIFLDVSTVVIISLVLFSQNTNLFFITLLALPIYTV
IIFAFMKPFEKMNRDTMEANAVLSSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQKALKKVAHL
LLNVGILWMGAVLVMDGKMSLGQLITYNTLLVYFTNPLENIINLQTKLQTAQVANNRLNEVYLVASEFEEKKTVEDLSLM
KGDMTFKQVSYKYGYGRDVLLDINLTIPQGSKVAFVGISGSGKTTLAKMMVNFYDPSQGEISLGGVNLNQIDKKALRQYI
NYLPQQPYVFNGTILDNLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGISGGQRQRIALARALLTDA
PVLILDEATSSLDILTEKRIVDNLMALDKTLIFIAHRLTIAERTEKVVVLDQGKIVEEGNHADLLAQGGFYAHLVNS

Nucleotide

• Length: 2154 bp

>NTDB_id=483 SM12261_RS00275 WP_000668315.1 54785..56938(+) (comA) [Streptococcus mitis NCTC 12261]
ATGAAATTTGGGAAACGTCACTATCGTCCGCAAGTGGATCAGATGGACTGCGGTGTAGCTTCATTAGCCATGGTTTTTGG
TTACTATGGTAGTTATTATTCTTTGGCACACTTACGAGAATTGGCCAAGACGACCATGGATGGGACGACGGCTTTGGGCT
TGGTCAAGGTGGCAGAGGAGATTGGTTTTGAGACGCGAGCAATTAAGGCGGATATGACGCTTTTTGACTTGCCAGATTTG
ACTTTTCCTTTTGTTGCTCATGTGCTTAAGGAAGGGAAATTGCTCCACTACTATGTGGTGACTGGGCAGGACAAGGATAG
CATTCATATTGCCGATCCAGATCCTGGGGTGAAATTGACCAAACTGCCACGTGAGCGTTTTGCGGAAGAATGGACAGGAG
TGACTCTTTTTATGGCACCTAGTCCAGACTACAAGCCTCATAAGGATCAAAAGAATGGTCTGCTCTCTTTTATCCCTATA
TTAGTGAAGCAGCGTGGCTTGATTGCCAATATCGTTTTGGCAACACTCTTGGTAACCTTGATTAATATTGTGGGTTCTTA
TTATCTGCAGTCTATCATTGATACCTATGTGCCAGATCAGATGCGTTCGACGCTTGGGATTATTTCTATTGGGCTGGTGA
TTGTTTATATTCTTCAGCAGATCTTGTCTTACGCTCAGGAGTATCTCTTACTTGTTTTGGGGCAACGCTTATCGATTGAT
GTGATTTTGTCCTATATCAAGCATGTTTTTCACCTGCCTATGTCCTTCTTTGCGACACGCAGGACAGGGGAGATCGTGTC
TCGTTTTACAGATGCTAATAGTATCATTGATGCGCTGGCTTCGACCATTCTTTCGATTTTCCTAGATGTGTCAACGGTTG
TCATTATTTCCCTTGTTTTATTTTCACAAAATACCAATCTCTTTTTCATAACCTTATTGGCACTTCCTATCTATACAGTG
ATTATCTTTGCCTTTATGAAGCCGTTTGAAAAGATGAATCGGGATACCATGGAAGCCAATGCGGTTCTGTCTTCTTCTAT
CATTGAGGACATCAACGGTATTGAGACTATCAAGTCTTTGACCAGTGAAAGTCAGCGTTACCAAAAGATTGACAAGGAAT
TTGTGGATTATCTGAAAAAATCCTTTACCTATAGTCGGGCAGAGAGTCAGCAAAAGGCTCTGAAAAAAGTTGCCCATCTC
TTACTCAATGTCGGCATTCTCTGGATGGGAGCTGTTCTGGTCATGGATGGCAAGATGAGTTTGGGGCAGTTGATTACCTA
TAATACCTTGCTGGTTTACTTTACAAATCCTTTGGAAAATATCATCAATCTGCAAACCAAGCTTCAGACAGCACAGGTTG
CCAATAACCGTCTAAATGAAGTATATCTGGTAGCTTCGGAGTTTGAGGAGAAGAAAACGGTTGAGGATTTGAGCTTGATG
AAGGGAGATATGACCTTCAAGCAGGTTTCTTACAAGTATGGCTATGGTCGAGACGTCTTGTTGGATATCAATTTGACCAT
TCCCCAAGGTTCTAAGGTGGCTTTTGTGGGGATTTCAGGGTCAGGTAAGACGACCTTGGCCAAGATGATGGTTAATTTTT
ACGACCCAAGTCAAGGGGAGATTAGTCTGGGTGGTGTCAATCTCAATCAGATTGATAAAAAGGCCTTGCGCCAGTATATC
AACTATCTGCCTCAACAGCCCTATGTCTTTAACGGAACGATTTTGGACAATCTTCTTTTGGGAGCCAAGGAGGGGACGAC
ACAGGAAGATATCTTACGGGCGGTCGAATTGGCAGAGATTCGGGAGGACATTGAGCGCATGCCACTGAATTACCAGACAG
AATTGACTTCGGATGGGGCAGGGATCTCAGGTGGTCAACGTCAGAGAATTGCTCTGGCACGTGCTCTCTTGACAGATGCG
CCGGTCTTGATCTTGGATGAGGCGACCAGCAGTTTGGATATTCTGACAGAGAAGCGAATTGTCGATAATCTTATGGCTTT
AGATAAGACCTTGATTTTCATCGCTCACCGCTTGACCATTGCTGAGCGGACAGAGAAGGTTGTTGTCTTGGATCAGGGCA
AGATTGTTGAAGAAGGAAATCATGCTGATTTGCTTGCACAGGGTGGTTTTTACGCCCATTTGGTCAATAGCTAG

Domains

Predicted by InterproScan.

• ABC transporter (ABC_tran) (502-650)

• Peptidase C39 family (Peptidase_C39) (7-142)

• ABC transporter transmembrane region (ABC_membrane) (170-438)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comA	Streptococcus pneumoniae R6	98.466	100	0.985
comA	Streptococcus mitis SK321	98.466	100	0.985
comA	Streptococcus pneumoniae Rx1	98.466	100	0.985
comA	Streptococcus pneumoniae D39	98.466	100	0.985
comA	Streptococcus pneumoniae TIGR4	98.047	100	0.98
comA	Streptococcus gordonii str. Challis substr. CH1	80.753	100	0.808
comA/nlmT	Streptococcus mutans UA159	65.411	100	0.654

References

• [1]: G Salvadori et al. (2018) High-resolution profiles of the Streptococcus mitis CSP signaling pathway reveal core and strain-specific regulated genes. BMC Genomics 19(1):453. [PMID: 29898666]