Detailed information    

experimental Experimentally validated

Overview

Name   comE   Type   Regulator
Locus tag   SM12261_RS09370 Genome accession   NZ_CP028414
Coordinates   1863638..1864390 (-) Length   250 a.a.
NCBI ID   WP_000866073.1    Uniprot ID   A0A081S8D7
Organism   Streptococcus mitis NCTC 12261     
Function   activate transcription of early competence genes   
Competence regulation

Function

Upregulated in response to CSP as measured by RNA-seq.


Genomic Context

Location: 1858638..1869390
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SM12261_RS09350 (SM12261_1804) - 1860083..1862725 (-) 2643 WP_000472064.1 YhgE/Pip domain-containing protein -
  SM12261_RS09355 (SM12261_1805) - 1862853..1863395 (+) 543 WP_001158274.1 TetR/AcrR family transcriptional regulator -
  SM12261_RS09370 (SM12261_1808) comE 1863638..1864390 (-) 753 WP_000866073.1 competence system response regulator transcription factor ComE Regulator
  SM12261_RS09375 (SM12261_1809) comD 1864387..1865712 (-) 1326 WP_020902616.1 competence system sensor histidine kinase ComD Regulator
  SM12261_RS09380 (SM12261_1810) comC 1865725..1865847 (-) 123 WP_004238992.1 competence-stimulating peptide ComC Regulator
  SM12261_RS09390 (SM12261_1812) rlmH 1866130..1866609 (-) 480 WP_000695934.1 23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH -
  SM12261_RS09395 (SM12261_1813) htrA 1866792..1867973 (+) 1182 WP_000681587.1 S1C family serine protease Regulator
  SM12261_RS09400 (SM12261_1814) spo0J 1868031..1868789 (+) 759 WP_000410370.1 ParB/RepB/Spo0J family partition protein Regulator

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  comE comX/sigX/comX2/sigX2 positive effect
  comX/sigX/comX2/sigX2 late competence genes positive effect
  comX/sigX/comX2/sigX2 late competence genes positive effect
  comX/sigX/comX1/sigX1 late competence genes positive effect
  comE comD positive effect
  comE comE positive effect
  comE comX/sigX/comX1/sigX1 positive effect
  comE comB positive effect
  comE comC positive effect
  comE comM positive effect
  comE comW positive effect
  comE comA positive effect
  comD comE positive effect
  comW comX/sigX/comX2/sigX2 positive effect
  comW comX/sigX/comX1/sigX1 positive effect
  comX/sigX/comX1/sigX1 late competence genes positive effect
  comC comD positive effect
  comB comC positive effect
  comA comC positive effect
  comM cbpD negative effect

Sequence

Protein


Download         Length: 250 a.a.        Molecular weight: 29947.32 Da        Isoelectric Point: 6.9819

>NTDB_id=487 SM12261_RS09370 WP_000866073.1 1863638..1864390(-) (comE) [Streptococcus mitis NCTC 12261]
MKVLILEDVIEHQVRLERILDEISKESNIPISYKTTGKVREFKEYIENDEVNQLYFLDIDIHGIEKKGFEVAQFIRHHNP
YAIIVFITSRSEFATLTYKYQVSALDFVDKDINDELFKKRIEQNIFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILY
IETTGVSHKLRIIGKNFAKEFYGTMTDIQEKDKHTQRFYSPHKSFLVNIGNIREIDRKNLEIVFYEDHRCPISRLKIRKL
KDILEKKSQK

Nucleotide


Download         Length: 753 bp        

>NTDB_id=487 SM12261_RS09370 WP_000866073.1 1863638..1864390(-) (comE) [Streptococcus mitis NCTC 12261]
ATGAAAGTTTTAATTTTAGAAGATGTTATTGAACATCAAGTGAGACTAGAGAGAATATTGGATGAAATTTCGAAAGAATC
GAATATTCCAATATCATACAAGACAACGGGAAAAGTTCGTGAGTTTAAGGAATATATCGAAAATGATGAAGTAAACCAGC
TTTATTTTCTAGATATTGATATTCATGGAATTGAGAAAAAAGGGTTTGAAGTTGCTCAGTTTATTCGTCATCACAATCCT
TACGCTATTATCGTCTTTATCACCAGTCGATCAGAGTTTGCGACTCTAACCTATAAATACCAGGTATCAGCCTTAGATTT
TGTTGATAAGGATATCAATGATGAGTTGTTTAAGAAGAGAATTGAGCAAAATATCTTCTACACGAAGAGTATGTTACTTG
AAAATGAAGATGTTGTAGATTATTTTGACTACAATTACAAGGGAAATGATTTAAAAATTCCTTACCATGATATTTTGTAT
ATTGAAACGACAGGTGTCTCTCATAAATTACGCATTATTGGTAAGAATTTTGCCAAAGAGTTTTACGGTACCATGACAGA
TATTCAGGAAAAGGACAAACATACTCAGCGATTTTATTCTCCTCATAAGTCATTTTTGGTAAATATAGGCAATATCAGAG
AAATTGATCGAAAAAACTTAGAAATTGTTTTCTATGAAGACCATCGTTGTCCTATTTCAAGATTAAAAATTAGAAAATTA
AAAGATATTCTAGAGAAAAAATCTCAAAAGTGA

Domains

Predicted by InterproScan.

Response regulator receiver domain (Response_reg)

(3-121)

LytTr DNA-binding domain (LytTR)

(151-244)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A081S8D7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Streptococcus mitis SK321

99.2

100

0.992
  comE Streptococcus pneumoniae TIGR4

98.4

100

0.984
  comE Streptococcus pneumoniae R6

98.4

100

0.984
  comE Streptococcus pneumoniae D39

98.4

100

0.984
  comE Streptococcus pneumoniae Rx1

98.4

100

0.984
  comE Streptococcus infantis strain Atu-4

92.8

100

0.928
  comE/comE1 Streptococcus gordonii str. Challis substr. CH1

64

100

0.64
  comE/comE2 Streptococcus gordonii strain NCTC7865

64

100

0.64
  comE/blpR Streptococcus mutans UA159

41.7

98.8

0.412
  comE/comE2 Streptococcus equinus JB1

35.039

100

0.365

Multiple sequence alignment    



References

[1] G Salvadori et al. (2018) High-resolution profiles of the Streptococcus mitis CSP signaling pathway reveal core and strain-specific regulated genes. BMC Genomics 19(1):453. [PMID: 29898666]