Detailed information    

experimental Experimentally validated

Overview

Name   comM   Type   Regulator
Locus tag   SM12261_RS08340 Genome accession   NZ_CP028414
Coordinates   1651347..1651979 (-) Length   210 a.a.
NCBI ID   WP_000970273.1    Uniprot ID   -
Organism   Streptococcus mitis NCTC 12261     
Function   fratricide immunity   
Competence regulation

Function

Upregulated in response to CSP as measured by RNA-seq.


Genomic Context

Location: 1646347..1656979
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SM12261_RS08315 (SM12261_1604) recA 1646694..1647848 (-) 1155 WP_078228268.1 recombinase RecA Machinery gene
  SM12261_RS08320 (SM12261_1605) cinA 1647903..1649159 (-) 1257 WP_000642690.1 competence/damage-inducible protein A Machinery gene
  SM12261_RS08325 (SM12261_1606) brpA 1649237..1650292 (-) 1056 WP_000239266.1 biofilm formation/cell division transcriptional regulator BrpA -
  SM12261_RS08330 (SM12261_1607) - 1650300..1650818 (-) 519 WP_000455549.1 GNAT family N-acetyltransferase -
  SM12261_RS08335 (SM12261_1608) tsaE 1650808..1651251 (-) 444 WP_000288249.1 tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE -
  SM12261_RS08340 (SM12261_1609) comM 1651347..1651979 (-) 633 WP_000970273.1 hypothetical protein Regulator
  SM12261_RS08345 (SM12261_1610) ndk 1652109..1652531 (-) 423 WP_004239114.1 nucleoside-diphosphate kinase -
  SM12261_RS08350 (SM12261_1611) rpoC 1652837..1656514 (-) 3678 WP_000228758.1 DNA-directed RNA polymerase subunit beta' -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  comM cbpD negative effect
  comE comM positive effect
  comE comX/sigX/comX2/sigX2 positive effect
  comX/sigX/comX2/sigX2 late competence genes positive effect
  comW comX/sigX/comX2/sigX2 positive effect
  comE comD positive effect
  comD comE positive effect
  comC comD positive effect
  comE comE positive effect
  comE comX/sigX/comX1/sigX1 positive effect
  comE comB positive effect
  comE comC positive effect
  comE comW positive effect
  comE comA positive effect
  comX/sigX/comX1/sigX1 late competence genes positive effect
  comW comX/sigX/comX1/sigX1 positive effect
  comB comC positive effect
  comA comC positive effect
  comX/sigX/comX2/sigX2 late competence genes positive effect
  comX/sigX/comX1/sigX1 late competence genes positive effect

Sequence

Protein


Download         Length: 210 a.a.        Molecular weight: 24055.23 Da        Isoelectric Point: 9.2991

>NTDB_id=490 SM12261_RS08340 WP_000970273.1 1651347..1651979(-) (comM) [Streptococcus mitis NCTC 12261]
MMEKMKKQIRVLLLLTVFGLSSCSCLSLSTWALMLNQNCFYYLVGMLLLASIGAGLNYYRGQGNQHYSVFKEDKQQFLYS
LLLLVNILGLALIVIEHILAGNTSVEGFVEAFLPSFFFLFGIDLLVFLPIQKYVNGVKKFLGKKEICFISGLAILIFLRN
PWMLLSMTFYIALGMLFLGLLVPKVIRWEVSFYSHLMRDILLVVFLLFLF

Nucleotide


Download         Length: 633 bp        

>NTDB_id=490 SM12261_RS08340 WP_000970273.1 1651347..1651979(-) (comM) [Streptococcus mitis NCTC 12261]
ATGATGGAAAAAATGAAAAAGCAAATTCGAGTTTTGTTATTGCTGACAGTTTTTGGTCTTAGCAGCTGTTCTTGTTTAAG
TTTATCTACTTGGGCCTTGATGTTAAATCAAAACTGCTTCTATTATTTAGTTGGCATGTTGCTTCTTGCCAGTATTGGTG
CAGGGCTAAACTATTATAGAGGGCAGGGAAATCAACATTATTCAGTTTTTAAGGAAGATAAGCAACAATTCTTGTATAGT
CTCTTGCTCTTAGTAAATATTTTGGGTTTAGCTTTAATTGTCATAGAACATATCCTTGCAGGAAATACATCCGTAGAAGG
ATTTGTGGAAGCCTTTCTACCGAGTTTCTTTTTCTTATTTGGGATTGATTTGTTAGTTTTTCTGCCGATTCAGAAGTATG
TAAATGGAGTGAAAAAGTTTTTGGGAAAGAAGGAAATCTGTTTTATTAGTGGACTAGCTATTCTAATCTTTCTCAGAAAT
CCTTGGATGCTATTGTCCATGACCTTTTATATTGCCTTGGGGATGCTATTTCTAGGACTGTTGGTGCCAAAAGTCATTCG
CTGGGAAGTTTCATTTTACAGTCATTTGATGAGAGATATCTTGTTAGTTGTCTTCTTACTGTTTCTATTTTAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Streptococcus mitis SK321

92.965

95.215

0.885
  comM Streptococcus pneumoniae TIGR4

39.024

97.619

0.381
  comM Streptococcus pneumoniae D39

39.024

97.619

0.381
  comM Streptococcus pneumoniae R6

39.024

97.619

0.381

Multiple sequence alignment    



References

[1] G Salvadori et al. (2018) High-resolution profiles of the Streptococcus mitis CSP signaling pathway reveal core and strain-specific regulated genes. BMC Genomics 19(1):453. [PMID: 29898666]