Detailed information    

experimental Experimentally validated

Overview

Name   comM   Type   Regulator
Locus tag   SMSK321_RS11040 Genome accession   NZ_AEDT01000034
Coordinates   622..1251 (+) Length   209 a.a.
NCBI ID   WP_000413917.1    Uniprot ID   -
Organism   Streptococcus mitis SK321     
Function   fratricide immunity   
Competence regulation

Function

Upregulated in response to CSP as measured by RNA-seq.


Genomic Context

Location: 1..6251
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SMSK321_RS11035 (SMSK321_1652) ndk 67..489 (+) 423 WP_000438301.1 nucleoside-diphosphate kinase -
  SMSK321_RS11040 (SMSK321_1653) comM 622..1251 (+) 630 WP_000413917.1 hypothetical protein Regulator
  SMSK321_RS11045 (SMSK321_1654) tsaE 1348..1791 (+) 444 WP_000288250.1 tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE -
  SMSK321_RS11050 (SMSK321_1655) - 1781..2299 (+) 519 WP_000455546.1 GNAT family N-acetyltransferase -
  SMSK321_RS11055 (SMSK321_1656) brpA 2307..3359 (+) 1053 WP_000239273.1 biofilm formation/cell division transcriptional regulator BrpA -
  SMSK321_RS11060 (SMSK321_1657) cinA 3448..4704 (+) 1257 WP_000642686.1 competence/damage-inducible protein A Machinery gene
  SMSK321_RS11065 (SMSK321_1658) recA 4759..5913 (+) 1155 WP_001085454.1 recombinase RecA Machinery gene

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  comM cbpD negative effect
  comE comM positive effect
  comE comE positive effect
  comE comX/sigX/comX1/sigX1 positive effect
  comE comC positive effect
  comE comD positive effect
  comE comA positive effect
  comE comX/sigX/comX2/sigX2 positive effect
  comE comW positive effect
  comE comB positive effect
  comD comE positive effect
  comX/sigX/comX1/sigX1 late competence genes positive effect
  comW comX/sigX/comX1/sigX1 positive effect
  comC comD positive effect
  comB comC positive effect
  comA comC positive effect
  comX/sigX/comX2/sigX2 late competence genes positive effect
  comW comX/sigX/comX2/sigX2 positive effect
  comX/sigX/comX1/sigX1 late competence genes positive effect
  comX/sigX/comX2/sigX2 late competence genes positive effect

Sequence

Protein


Download         Length: 209 a.a.        Molecular weight: 23933.09 Da        Isoelectric Point: 9.6680

>NTDB_id=518 SMSK321_RS11040 WP_000413917.1 622..1251(+) (comM) [Streptococcus mitis SK321]
MEKMKKQIRVLLLFTIFGISSCSCLSLSTWALMLNQNCFYYLVGMLLLASIGSGINYYSGQRNQSYSVFKEDKQQFLYSL
LLLVNILGLALIVIDHILAGNASVEGFVEAFLPSFFFLFGIDLLVFLPIQKYVNGLKKILGKKKICFISGLAILIFLRNP
WMLLSMTFYITLGMLFLGLLVPKVIRWEVSFYSHLMRDILLVVFLLFLF

Nucleotide


Download         Length: 630 bp        

>NTDB_id=518 SMSK321_RS11040 WP_000413917.1 622..1251(+) (comM) [Streptococcus mitis SK321]
ATGGAAAAAATGAAAAAGCAAATTCGTGTTTTGTTATTGTTTACGATTTTTGGTATTAGTAGTTGTTCTTGTTTAAGTTT
ATCGACTTGGGCCTTGATGTTAAATCAAAACTGCTTCTATTATTTAGTTGGCATGTTGCTCCTTGCCAGTATTGGTTCAG
GGATAAACTACTATAGTGGACAGCGAAATCAATCTTATTCAGTTTTTAAGGAAGATAAGCAACAATTCTTGTATAGTCTG
TTGCTCTTAGTAAACATTTTGGGTTTAGCTTTAATTGTCATAGACCATATCCTTGCAGGAAATGCATCCGTAGAAGGGTT
TGTGGAAGCCTTTCTACCGAGTTTCTTTTTCTTATTTGGGATTGATTTGCTAGTCTTTTTACCGATTCAGAAGTATGTAA
ATGGATTGAAAAAGATTTTGGGAAAGAAGAAAATCTGTTTTATTAGTGGACTAGCTATTCTAATCTTTCTCAGAAATCCT
TGGATGCTATTGTCCATGACCTTTTATATTACCTTAGGAATGCTATTTCTAGGACTGTTGGTGCCAAAAGTCATTCGCTG
GGAAGTTTCATTTTACAGTCATTTGATGAGAGATATCTTGTTAGTTGTCTTCTTACTGTTTCTATTTTAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Streptococcus mitis NCTC 12261

92.965

95.215

0.885
  comM Streptococcus pneumoniae D39

39.024

98.086

0.383
  comM Streptococcus pneumoniae TIGR4

39.024

98.086

0.383
  comM Streptococcus pneumoniae R6

39.024

98.086

0.383

Multiple sequence alignment    



References

[1] G Salvadori et al. (2018) High-resolution profiles of the Streptococcus mitis CSP signaling pathway reveal core and strain-specific regulated genes. BMC Genomics 19(1):453. [PMID: 29898666]