Detailed information    

experimental Experimentally validated

Overview

Name   comW   Type   Regulator
Locus tag   SMSK321_RS07095 Genome accession   NZ_AEDT01000020
Coordinates   460..696 (+) Length   78 a.a.
NCBI ID   WP_000941922.1    Uniprot ID   -
Organism   Streptococcus mitis SK321     
Function   stabilization and activation of ComX   
Competence regulation

Function

Upregulated in response to CSP as measured by RNA-seq.


Genomic Context

Location: 1..5696
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SMSK321_RS07095 (SMSK321_0337) comW 460..696 (+) 237 WP_000941922.1 sigma(X)-activator ComW Regulator
  SMSK321_RS07100 (SMSK321_0338) - 927..2213 (+) 1287 WP_001832534.1 adenylosuccinate synthase -
  SMSK321_RS07105 (SMSK321_0339) tadA 2413..2880 (+) 468 WP_001110114.1 tRNA adenosine(34) deaminase TadA -
  SMSK321_RS07110 (SMSK321_0340) - 3067..3510 (+) 444 WP_000701987.1 dUTP diphosphatase -
  SMSK321_RS07115 (SMSK321_0341) - 3470..4027 (+) 558 WP_000863560.1 histidine phosphatase family protein -
  SMSK321_RS07120 (SMSK321_0342) radA 4041..5402 (+) 1362 WP_080550752.1 DNA repair protein RadA Machinery gene

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  comW comX/sigX/comX1/sigX1 positive effect
  comX/sigX/comX1/sigX1 late competence genes positive effect
  comX/sigX/comX1/sigX1 late competence genes positive effect
  comX/sigX/comX2/sigX2 late competence genes positive effect
  comE comX/sigX/comX1/sigX1 positive effect
  comE comE positive effect
  comE comM positive effect
  comE comC positive effect
  comE comD positive effect
  comE comA positive effect
  comE comX/sigX/comX2/sigX2 positive effect
  comE comW positive effect
  comE comB positive effect
  comD comE positive effect
  comW comX/sigX/comX2/sigX2 positive effect
  comX/sigX/comX2/sigX2 late competence genes positive effect
  comM cbpD negative effect
  comC comD positive effect
  comB comC positive effect
  comA comC positive effect

Sequence

Protein


Download         Length: 78 a.a.        Molecular weight: 9942.55 Da        Isoelectric Point: 6.7123

>NTDB_id=519 SMSK321_RS07095 WP_000941922.1 460..696(+) (comW) [Streptococcus mitis SK321]
MLQSIYDQMMDFYRNIEEEYGMFFGDNFDWEYVHFKFLIYYLVRYRIVSHRDFIVYHYRVAYRLYLEKLIMKQGFVAC

Nucleotide


Download         Length: 237 bp        

>NTDB_id=519 SMSK321_RS07095 WP_000941922.1 460..696(+) (comW) [Streptococcus mitis SK321]
ATGCTACAAAGTATTTATGATCAGATGATGGATTTTTATAGAAATATTGAAGAAGAGTATGGCATGTTCTTCGGTGATAA
TTTTGATTGGGAATATGTTCATTTTAAGTTTTTGATTTATTATCTTGTCCGATATCGTATTGTGAGTCATCGGGATTTTA
TTGTTTACCATTATCGTGTTGCTTATCGTTTGTATCTTGAAAAATTGATAATGAAACAAGGTTTTGTTGCTTGTTGA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comW Streptococcus mitis NCTC 12261

97.403

100

0.974
  comW Streptococcus pneumoniae R6

75.641

100

0.756
  comW Streptococcus pneumoniae D39

75.641

100

0.756
  comW Streptococcus pneumoniae Rx1

75.641

100

0.756
  comW Streptococcus pneumoniae TIGR4

75.641

100

0.756

Multiple sequence alignment    



References

[1] G Salvadori et al. (2018) High-resolution profiles of the Streptococcus mitis CSP signaling pathway reveal core and strain-specific regulated genes. BMC Genomics 19(1):453. [PMID: 29898666]