Detailed information    

experimental Experimentally validated

Overview

Name   comB   Type   Regulator
Locus tag   SMSK321_RS10735 Genome accession   NZ_AEDT01000033
Coordinates   5493..6842 (+) Length   449 a.a.
NCBI ID   WP_000801634.1    Uniprot ID   -
Organism   Streptococcus mitis SK321     
Function   transport of ComC   
Competence regulation

Function

Upregulated in response to CSP as measured by RNA-seq.


Genomic Context

Location: 493..11842
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SMSK321_RS10710 (SMSK321_1305) - 1506..1703 (+) 198 WP_000180825.1 class IIb bacteriocin, lactobin A/cerein 7B family -
  SMSK321_RS10715 (SMSK321_1306) - 1917..2072 (+) 156 WP_000357248.1 smi_0061 family bacteriocin-like peptide -
  SMSK321_RS12550 - 2076..2291 (+) 216 WP_080550793.1 bacteriocin immunity protein -
  SMSK321_RS10720 (SMSK321_1308) - 2372..2551 (+) 180 WP_000846993.1 hypothetical protein -
  SMSK321_RS12555 (SMSK321_1309) - 2980..3168 (+) 189 WP_000757184.1 hypothetical protein -
  SMSK321_RS10730 (SMSK321_1310) comA 3327..5480 (+) 2154 WP_000668322.1 peptide cleavage/export ABC transporter ComA Regulator
  SMSK321_RS10735 (SMSK321_1311) comB 5493..6842 (+) 1350 WP_000801634.1 competence pheromone export protein ComB Regulator
  SMSK321_RS10740 (SMSK321_1312) purC 7012..7719 (+) 708 WP_000043295.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
  SMSK321_RS12650 - 7721..7864 (+) 144 WP_079211609.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
  SMSK321_RS10745 (SMSK321_1313) - 7921..11646 (+) 3726 WP_000361185.1 phosphoribosylformylglycinamidine synthase -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  comB comC positive effect
  comC comD positive effect
  comD comE positive effect
  comE comE positive effect
  comE comM positive effect
  comE comX/sigX/comX1/sigX1 positive effect
  comE comC positive effect
  comE comD positive effect
  comE comA positive effect
  comE comX/sigX/comX2/sigX2 positive effect
  comE comW positive effect
  comE comB positive effect
  comM cbpD negative effect
  comX/sigX/comX1/sigX1 late competence genes positive effect
  comW comX/sigX/comX1/sigX1 positive effect
  comA comC positive effect
  comX/sigX/comX2/sigX2 late competence genes positive effect
  comW comX/sigX/comX2/sigX2 positive effect
  comX/sigX/comX1/sigX1 late competence genes positive effect
  comX/sigX/comX2/sigX2 late competence genes positive effect

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 49946.93 Da        Isoelectric Point: 5.5749

>NTDB_id=512 SMSK321_RS10735 WP_000801634.1 5493..6842(+) (comB) [Streptococcus mitis SK321]
MKPEFLESAEFYNRRYHTFSSRVIVPMSLLLVFLLGFATFAEKEMSLSTRATVEPSRILANIQSTSNNRILVNHLEENKL
VKKGELLVQYQEGAEGVQAEAYASQLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQN
ETIASQNAAASQTQAEIGNLISQTEAKIRDYQTAKSAIETGASLASQNLAYSLYQSYKSQGEENPQAKAQAVAQVEAQLS
QLESSLTTYRVQYAGSGIQQAYASGLSSQLESLKSQHLAKVSQELTLLDQKILEVESGKKVQGNLLDKGKITASEDGVLH
LNPETSDSTMVAEGTLLAQLYPSLEKEGKAKLTAYLSSKDVARIKVGDSVRYTTTHDAKNQIFLDSTITSIDATATKTEK
GNFFKIEAETNLTSEQTEKLRYGVEGRLQMITGRKSYLRYYLDQFLNKE

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=512 SMSK321_RS10735 WP_000801634.1 5493..6842(+) (comB) [Streptococcus mitis SK321]
ATGAAACCAGAATTTTTAGAAAGTGCGGAGTTTTATAATCGTCGTTACCATACTTTTTCCAGTCGGGTGATTGTACCCAT
GTCCCTTCTGCTCGTGTTTTTGCTTGGATTTGCAACATTTGCAGAGAAGGAGATGAGTTTGTCTACTAGAGCTACTGTCG
AACCTAGTCGTATCCTTGCAAATATCCAGTCAACTAGCAACAATCGCATTCTTGTCAATCATTTGGAAGAAAATAAGCTG
GTTAAGAAGGGGGAGCTGTTGGTTCAATATCAGGAAGGGGCAGAGGGTGTCCAAGCGGAGGCCTATGCTAGTCAGTTGGA
CATGCTAAAGGACCAAAAAAAGCAATTGGAGTATTTGCAAAAAAGTCTGCAAGAAGGGGAGAACCACTTTCCAGAGGAGG
ATAAGTTTGGTTACCAAGCCACCTTTCGCGACTACATCAGTCAAGCAGGCAGTCTTAGGGCTAGTACATCGCAACAAAAT
GAGACCATCGCGTCCCAGAATGCAGCAGCTAGCCAAACCCAAGCCGAAATCGGCAACCTCATCAGCCAAACAGAGGCTAA
AATTCGCGATTACCAGACAGCTAAGTCAGCTATTGAAACAGGTGCTTCCTTGGCCAGTCAGAATCTAGCCTACTCTCTCT
ACCAGTCCTACAAGTCTCAAGGCGAGGAAAATCCGCAAGCTAAGGCTCAGGCTGTTGCGCAGGTAGAAGCGCAGCTTTCT
CAGTTAGAATCTAGCCTTACTACTTACCGTGTCCAGTATGCAGGTTCAGGTATCCAGCAAGCCTATGCGTCAGGTTTAAG
TAGTCAATTGGAGTCTCTCAAATCCCAACACTTGGCAAAGGTTAGTCAGGAATTGACCCTTCTAGACCAGAAAATCTTGG
AGGTGGAGTCAGGTAAGAAGGTACAGGGAAATCTTTTAGACAAGGGGAAAATTACGGCCAGTGAGGATGGGGTGCTTCAT
CTTAATCCTGAGACCAGTGATTCTACCATGGTTGCAGAGGGGACTCTACTAGCCCAACTCTATCCGTCCTTGGAAAAAGA
AGGGAAAGCCAAACTAACAGCTTATCTTAGTTCAAAAGATGTAGCAAGGATCAAGGTCGGTGATTCTGTTCGCTATACGA
CGACTCATGATGCCAAGAATCAAATTTTCCTAGATTCTACTATTACAAGTATTGATGCGACAGCTACTAAGACTGAAAAA
GGGAATTTCTTTAAAATCGAGGCGGAGACTAATCTGACTTCCGAGCAGACTGAAAAACTTCGATACGGGGTGGAAGGTCG
TCTGCAGATGATTACGGGCAGGAAAAGTTATCTACGTTATTATTTGGATCAATTTTTGAACAAAGAGTAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comB Streptococcus mitis NCTC 12261

95.323

100

0.953
  comB Streptococcus pneumoniae Rx1

94.878

100

0.949
  comB Streptococcus pneumoniae D39

94.878

100

0.949
  comB Streptococcus pneumoniae R6

94.878

100

0.949
  comB Streptococcus pneumoniae TIGR4

94.655

100

0.947
  comB Streptococcus gordonii str. Challis substr. CH1

55.111

100

0.552

Multiple sequence alignment    



References

[1] G Salvadori et al. (2018) High-resolution profiles of the Streptococcus mitis CSP signaling pathway reveal core and strain-specific regulated genes. BMC Genomics 19(1):453. [PMID: 29898666]