Detailed information    

experimental Experimentally validated

Overview

Name   comB   Type   Regulator
Locus tag   KZH43_RS00225 Genome accession   NZ_CP079923
Coordinates   44068..45417 (+) Length   449 a.a.
NCBI ID   WP_000801613.1    Uniprot ID   A0A2U3RX82
Organism   Streptococcus pneumoniae Rx1     
Function   transport of ComC   
Competence regulation

Function

ComAB transporter secret the mature competence-stimulating peptide (CSP) into the extracellular environment. This secretion is a critical step in the quorum-sensing mechanism that triggers competence development. Once CSP reaches a threshold concentration, it activates the ComDE two-component system, leading to the expression of early competence genes and eventually the late competence genes required for DNA uptake and recombination.


Genomic Context

Location: 39068..50417
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KZH43_RS00210 (KZH43_00210) blpU 39514..39744 (+) 231 WP_001093075.1 bacteriocin-like peptide BlpU -
  KZH43_RS10285 - 39747..39872 (+) 126 WP_000346297.1 PncF family bacteriocin immunity protein -
  KZH43_RS00220 (KZH43_00220) comA 41902..44055 (+) 2154 WP_000668290.1 peptide cleavage/export ABC transporter ComA Regulator
  KZH43_RS00225 (KZH43_00225) comB 44068..45417 (+) 1350 WP_000801613.1 competence pheromone export protein ComB Regulator
  KZH43_RS00230 (KZH43_00230) purC 45587..46294 (+) 708 WP_000043299.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
  KZH43_RS10755 - 46296..46439 (+) 144 WP_050167432.1 hypothetical protein -
  KZH43_RS00235 (KZH43_00235) - 46496..50221 (+) 3726 WP_000361172.1 phosphoribosylformylglycinamidine synthase -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  comB comC/comC1 positive effect
  comC/comC1 comD/comD1 positive effect
  comD/comD1 comE positive effect
  comE comA positive effect
  comA comC/comC1 positive effect
  comE comD/comD1 positive effect
  comE comB positive effect
  ciaR comC/comC1 negative effect
  ciaR htrA positive effect
  ciaH comC/comC1 negative effect
  ciaH htrA positive effect
  comE comC/comC1 positive effect
  comE comE positive effect
  comE comX/comX1 positive effect
  comE comX/comX2 positive effect
  comE comM positive effect
  comE comW positive effect
  stkP comE positive effect
  htrA comC/comC1 negative effect
  htrA comEA/celA/cilE negative effect
  htrA comEC/celB negative effect
  comX/comX1 late competence genes positive effect
  comW comX/comX1 positive effect
  clpE comX/comX1 negative effect
  clpP comX/comX1 negative effect
  comX/comX2 late competence genes positive effect
  comW comX/comX2 positive effect
  clpE comX/comX2 negative effect
  clpP comX/comX2 negative effect
  comM cbpD negative effect
  clpC comW negative effect
  clpP comW negative effect
  mecA comW negative effect
  comX/comX1 late competence genes positive effect
  comX/comX2 late competence genes positive effect
  cbpD lytA positive effect
  cbpD lytC positive effect

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 49613.45 Da        Isoelectric Point: 5.3895

>NTDB_id=248 KZH43_RS00225 WP_000801613.1 44068..45417(+) (comB) [Streptococcus pneumoniae Rx1]
MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKEMSLSTRATVEPSRILANIQSTSNNRILVNHLEENKL
VKKGDLLVQYQEGAEGVQAESYASQLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQN
ETIASQNAAASQTQAEIGNLISQTEAKIRDYQTAKSAIETGTSLAGQNLAYSLYQSYKSQGEENPQTKVQAVAQVEAQIS
QLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQHLAKVGQELSLLAQKILEAESGKKVQGNLLDKGKITASEDGVLH
LNPETSDSSMVAEGTLLAQLYPSLEREGKAKLTAYLSSKDVARIKVGDSVRYTTTHDAGNQLFLDSTITSIDATATKTEK
GNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=248 KZH43_RS00225 WP_000801613.1 44068..45417(+) (comB) [Streptococcus pneumoniae Rx1]
ATGAAACCAGAATTTTTAGAAAGTGCGGAGTTTTATAATCGTCGTTACCATAATTTTTCCAGTAGTGTGATTGTACCCAT
GGCCCTTCTGCTCGTGTTTTTACTTGGCTTTGCAACTGTTGCAGAGAAGGAGATGAGTTTGTCCACTAGAGCTACTGTCG
AACCCAGTCGTATCCTTGCAAATATCCAGTCAACTAGCAACAATCGTATTCTTGTCAATCATTTGGAAGAAAATAAGCTG
GTTAAGAAGGGGGATCTTTTGGTTCAATACCAAGAAGGGGCAGAGGGTGTCCAAGCGGAGTCCTATGCCAGTCAGTTGGA
CATGCTAAAGGATCAAAAAAAGCAATTGGAGTATCTGCAAAAGAGCCTGCAAGAAGGGGAGAACCACTTTCCAGAGGAGG
ATAAGTTTGGCTACCAAGCCACCTTTCGCGACTACATCAGTCAAGCAGGCAGTCTTAGGGCTAGTACATCGCAACAAAAT
GAGACCATCGCGTCCCAGAATGCAGCAGCTAGCCAAACCCAAGCCGAAATCGGCAACCTCATCAGTCAAACAGAGGCTAA
AATTCGCGATTACCAGACAGCTAAGTCAGCTATTGAAACAGGTACTTCCTTGGCCGGTCAGAATCTAGCCTACTCTCTTT
ACCAGTCCTACAAGTCTCAGGGCGAGGAAAATCCCCAAACTAAGGTTCAGGCAGTTGCACAGGTTGAAGCACAGATTTCT
CAGTTAGAATCTAGTCTTGCTACTTACCGTGTCCAGTATGCAGGTTCAGGTACCCAGCAAGCCTATGCGTCAGGGTTAAG
CAGTCAATTGGAATCCCTTAAATCCCAACACTTGGCAAAGGTTGGTCAGGAATTGAGCCTTCTAGCCCAGAAAATCTTGG
AGGCAGAGTCAGGTAAGAAGGTACAGGGAAATCTTTTAGACAAGGGGAAAATTACGGCGAGTGAGGATGGGGTGCTTCAT
CTTAATCCTGAGACCAGTGATTCTAGCATGGTTGCAGAAGGTACCCTACTAGCCCAACTTTATCCATCTTTGGAAAGAGA
AGGGAAAGCCAAACTCACAGCTTATCTAAGTTCAAAAGATGTAGCAAGAATCAAGGTCGGTGATTCTGTTCGCTATACTA
CGACTCATGATGCCGGGAATCAACTTTTCCTAGATTCTACTATTACAAGTATTGATGCGACAGCTACTAAGACTGAGAAA
GGGAATTTCTTTAAAATCGAGGCGGAGACTAATCTAACTTCGGAGCAGGCTGAAAAACTTAGGTACGGGGTGGAAGGCCG
CTTGCAGATGATTACGGGCAAGAAAAGTTACCTACGTTATTATTTAGATCAATTTTTGAACAAAGAGTAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2U3RX82

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comB Streptococcus pneumoniae D39

100

100

1
  comB Streptococcus pneumoniae R6

100

100

1
  comB Streptococcus pneumoniae TIGR4

98.886

100

0.989
  comB Streptococcus mitis SK321

94.878

100

0.949
  comB Streptococcus mitis NCTC 12261

93.987

100

0.94
  comB Streptococcus gordonii str. Challis substr. CH1

55.556

100

0.557

Multiple sequence alignment    



References

[1] L S Håvarstein et al. (1995) An unmodified heptadecapeptide pheromone induces competence for genetic transformation in Streptococcus pneumoniae. Proceedings of The National Academy of Sciences of The United States of America 92(24):11140-4. [PMID: 7479953]
[2] L S Håvarstein et al. (1995) A family of bacteriocin ABC transporters carry out proteolytic processing of their substrates concomitant with export. Molecular Microbiology 16(2):229-40. [PMID: 7565085]
[3] F M Hui et al. (1991) Genetic transformation in Streptococcus pneumoniae: nucleotide sequence analysis shows comA, a gene required for competence induction, to be a member of the bacterial ATP-dependent transport protein family. Journal of Bacteriology 173(1):372-81. [PMID: 1987129]