Detailed information    

experimental Experimentally validated

Overview

Name   comB   Type   Regulator
Locus tag   SM12261_RS00280 Genome accession   NZ_CP028414
Coordinates   56951..58300 (+) Length   449 a.a.
NCBI ID   WP_000801588.1    Uniprot ID   A0AAX2L2X7
Organism   Streptococcus mitis NCTC 12261     
Function   transport of ComC   
Competence regulation

Function

Upregulated in response to CSP as measured by RNA-seq.


Genomic Context

Location: 51951..63300
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SM12261_RS00250 (SM12261_0051) - 52314..53045 (+) 732 WP_001221869.1 response regulator transcription factor -
  SM12261_RS00255 (SM12261_0052) - 53291..53419 (+) 129 WP_000658220.1 smi_0059.1 family bacteriocin-like peptide -
  SM12261_RS09435 - 53431..53586 (+) 156 WP_001004642.1 hypothetical protein -
  SM12261_RS00265 (SM12261_0053) - 53620..53793 (+) 174 WP_000180829.1 class IIb bacteriocin, lactobin A/cerein 7B family -
  SM12261_RS00270 (SM12261_0054) - 53917..54069 (+) 153 WP_001180497.1 class IIb bacteriocin, lactobin A/cerein 7B family -
  SM12261_RS09440 (SM12261_0055) - 54094..54249 (+) 156 WP_000732248.1 hypothetical protein -
  SM12261_RS00275 (SM12261_0056) comA 54785..56938 (+) 2154 WP_000668315.1 peptide cleavage/export ABC transporter ComA Regulator
  SM12261_RS00280 (SM12261_0057) comB 56951..58300 (+) 1350 WP_000801588.1 competence pheromone export protein ComB Regulator
  SM12261_RS00285 (SM12261_0058) purC 58470..59177 (+) 708 WP_000043282.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
  SM12261_RS09700 - 59179..59322 (+) 144 WP_078228447.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
  SM12261_RS00295 (SM12261_0059) - 59377..63102 (+) 3726 WP_000361182.1 phosphoribosylformylglycinamidine synthase -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  comB comC positive effect
  comC comD positive effect
  comD comE positive effect
  comE comX/sigX/comX2/sigX2 positive effect
  comX/sigX/comX2/sigX2 late competence genes positive effect
  comX/sigX/comX2/sigX2 late competence genes positive effect
  comW comX/sigX/comX2/sigX2 positive effect
  comX/sigX/comX1/sigX1 late competence genes positive effect
  comX/sigX/comX1/sigX1 late competence genes positive effect
  comW comX/sigX/comX1/sigX1 positive effect
  comE comX/sigX/comX1/sigX1 positive effect
  comE comD positive effect
  comE comE positive effect
  comE comB positive effect
  comE comC positive effect
  comE comM positive effect
  comE comW positive effect
  comE comA positive effect
  comA comC positive effect
  comM cbpD negative effect

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 49831.90 Da        Isoelectric Point: 6.0333

>NTDB_id=484 SM12261_RS00280 WP_000801588.1 56951..58300(+) (comB) [Streptococcus mitis NCTC 12261]
MKPEFLESAEFYNRRYHNFSSRVIVPMFLLLVFLLGFATLAEKEMSMSTRATVEPSRILANIQSTSNNRILVNHLEESKL
VKKGELLVQYQEGAEGVQAEAYASQLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQN
ETIASQNAAASQTQAEIGTLISQTEAKIRDYQTAKSAIETGASLASQNLAYSLYQSYKSQGEENPQSKAQAVVQLEAQLS
QLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQHLAKVGQELTLLDQKILEAESGRKVQGNLLDKGKITASEDGVLH
LNPETSDSTMVAEGALLAQLYPSLEKEGKVKLTAYLSSKDVARIKVGDSVRYTTTHDAKNQLFLDSTITSIDATATKTET
GNFFKIEAETNLTSKQAAKLRYGVEGRLQMITGRKSYLRYYLDQFLNKE

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=484 SM12261_RS00280 WP_000801588.1 56951..58300(+) (comB) [Streptococcus mitis NCTC 12261]
ATGAAACCAGAATTTTTAGAAAGTGCGGAGTTTTATAATCGTCGTTACCATAATTTTTCCAGTCGGGTGATTGTCCCCAT
GTTCCTTCTGCTCGTGTTTTTACTTGGCTTTGCAACTTTGGCAGAGAAGGAGATGAGTATGTCTACTAGAGCTACTGTCG
AGCCTAGTCGTATCCTTGCAAATATCCAGTCAACTAGCAACAATCGTATTCTTGTCAATCATTTGGAAGAAAGTAAGCTG
GTTAAAAAGGGAGAACTGTTGGTTCAATATCAAGAAGGGGCAGAGGGTGTCCAAGCGGAGGCCTATGCTAGTCAGCTGGA
CATGCTCAAGGATCAAAAAAAGCAATTGGAGTATTTGCAAAAGAGTCTGCAAGAAGGGGAGAACCACTTTCCAGAGGAGG
ATAAATTTGGCTACCAAGCCACCTTTCGTGACTACATCAGCCAAGCAGGTAGTCTTAGGGCTAGTACATCGCAACAAAAT
GAGACCATCGCGTCCCAGAATGCAGCAGCTAGTCAAACCCAAGCCGAAATCGGCACCCTCATCAGTCAAACAGAGGCTAA
AATTCGCGATTACCAGACAGCTAAGTCAGCTATTGAAACAGGTGCTTCCTTGGCCAGTCAGAATCTAGCCTACTCTCTCT
ACCAGTCCTACAAGTCTCAGGGCGAGGAAAATCCGCAATCTAAGGCACAAGCAGTTGTGCAGCTAGAAGCACAGCTTTCT
CAGTTAGAATCTAGTCTTGCTACTTACCGTGTCCAGTATGCAGGTTCAGGTACCCAGCAAGCCTATGCGTCAGGTTTAAG
CAGTCAATTGGAGTCTCTCAAATCACAACACTTGGCTAAGGTTGGTCAGGAATTGACCCTTCTAGACCAGAAAATTTTGG
AGGCAGAATCGGGTAGGAAGGTACAGGGAAATCTTTTAGACAAGGGGAAAATTACGGCGAGTGAGGATGGGGTGCTTCAT
CTTAATCCTGAGACCAGTGATTCTACCATGGTAGCAGAAGGCGCCTTACTAGCCCAACTCTATCCGTCCTTGGAAAAAGA
AGGGAAAGTCAAACTCACAGCTTATCTAAGTTCAAAAGATGTAGCAAGGATCAAGGTCGGTGATTCTGTTCGCTATACTA
CGACTCATGATGCTAAGAATCAACTTTTCCTAGATTCTACTATTACAAGTATTGATGCGACAGCTACTAAGACTGAAACT
GGGAATTTCTTTAAAATCGAGGCGGAGACTAATCTAACTTCTAAGCAGGCTGCAAAACTTCGATATGGGGTGGAAGGTCG
CCTGCAGATGATTACGGGCAGGAAAAGTTACCTACGTTATTATTTGGATCAATTTTTGAACAAAGAGTAA

Domains

Predicted by InterproScan.

HlyD family secretion protein (HlyD_3)

(310-423)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comB Streptococcus mitis SK321

95.323

100

0.953
  comB Streptococcus pneumoniae TIGR4

94.209

100

0.942
  comB Streptococcus pneumoniae D39

93.987

100

0.94
  comB Streptococcus pneumoniae Rx1

93.987

100

0.94
  comB Streptococcus pneumoniae R6

93.987

100

0.94
  comB Streptococcus gordonii str. Challis substr. CH1

55.778

100

0.559

Multiple sequence alignment    



References

[1] G Salvadori et al. (2018) High-resolution profiles of the Streptococcus mitis CSP signaling pathway reveal core and strain-specific regulated genes. BMC Genomics 19(1):453. [PMID: 29898666]