Detailed information    

experimental Experimentally validated

Overview

Name   comW   Type   Regulator
Locus tag   SM12261_RS00100 Genome accession   NZ_CP028414
Coordinates   20603..20836 (+) Length   77 a.a.
NCBI ID   WP_000941923.1    Uniprot ID   -
Organism   Streptococcus mitis NCTC 12261     
Function   stabilization and activation of ComX   
Competence regulation

Function

Upregulated in response to CSP as measured by RNA-seq.


Genomic Context

Location: 15603..25836
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SM12261_RS00100 (SM12261_0022) comW 20603..20836 (+) 234 WP_000941923.1 sigma(X)-activator ComW Regulator
  SM12261_RS00105 (SM12261_0023) - 21070..22356 (+) 1287 WP_025169748.1 adenylosuccinate synthase -
  SM12261_RS00110 (SM12261_0024) tadA 22556..23023 (+) 468 WP_001110112.1 tRNA adenosine(34) deaminase TadA -
  SM12261_RS00120 (SM12261_0025) - 23210..23653 (+) 444 WP_000702005.1 dUTP diphosphatase -
  SM12261_RS00125 (SM12261_0026) - 23613..24170 (+) 558 WP_000863561.1 histidine phosphatase family protein -
  SM12261_RS00130 (SM12261_0027) radA 24184..25545 (+) 1362 WP_078228442.1 DNA repair protein RadA Machinery gene

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  comW comX/sigX/comX1/sigX1 positive effect
  comX/sigX/comX1/sigX1 late competence genes positive effect
  comX/sigX/comX2/sigX2 late competence genes positive effect
  comX/sigX/comX1/sigX1 late competence genes positive effect
  comX/sigX/comX2/sigX2 late competence genes positive effect
  comE comX/sigX/comX2/sigX2 positive effect
  comE comD positive effect
  comE comE positive effect
  comE comX/sigX/comX1/sigX1 positive effect
  comE comB positive effect
  comE comC positive effect
  comE comM positive effect
  comE comW positive effect
  comE comA positive effect
  comD comE positive effect
  comW comX/sigX/comX2/sigX2 positive effect
  comC comD positive effect
  comB comC positive effect
  comA comC positive effect
  comM cbpD negative effect

Sequence

Protein


Download         Length: 77 a.a.        Molecular weight: 9779.36 Da        Isoelectric Point: 6.8760

>NTDB_id=491 SM12261_RS00100 WP_000941923.1 20603..20836(+) (comW) [Streptococcus mitis NCTC 12261]
MLQSIYDQMMDFYRNIEEEYGMFLGDNFDWEHVHFKFLIYYLVRYRIVSHRDFIVYHYRVAYRLYLEKLIMKQGFVA

Nucleotide


Download         Length: 234 bp        

>NTDB_id=491 SM12261_RS00100 WP_000941923.1 20603..20836(+) (comW) [Streptococcus mitis NCTC 12261]
ATGCTACAAAGTATTTATGATCAGATGATGGATTTTTATAGAAATATTGAAGAAGAGTATGGTATGTTCTTGGGTGATAA
TTTTGATTGGGAACATGTTCATTTTAAGTTTTTGATTTATTATCTTGTCCGATATCGTATTGTGAGTCATCGGGATTTTA
TTGTTTACCATTATCGTGTTGCTTATCGTTTGTATCTTGAAAAATTGATAATGAAACAGGGTTTTGTTGCTTGA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comW Streptococcus mitis SK321

97.403

100

0.974
  comW Streptococcus pneumoniae TIGR4

75.325

100

0.753
  comW Streptococcus pneumoniae Rx1

75.325

100

0.753
  comW Streptococcus pneumoniae R6

75.325

100

0.753
  comW Streptococcus pneumoniae D39

75.325

100

0.753

Multiple sequence alignment    



References

[1] G Salvadori et al. (2018) High-resolution profiles of the Streptococcus mitis CSP signaling pathway reveal core and strain-specific regulated genes. BMC Genomics 19(1):453. [PMID: 29898666]