Detailed information    

experimental Experimentally validated

Overview

Name   comX/sigX/comX1/sigX1   Type   Regulator
Locus tag   SM12261_RS00065 Genome accession   NZ_CP028414
Coordinates   14370..14849 (+) Length   159 a.a.
NCBI ID   WP_000471939.1    Uniprot ID   -
Organism   Streptococcus mitis NCTC 12261     
Function   activate transcription of late competence genes   
Competence regulation

Function

Upregulated in response to CSP as measured by RNA-seq.


Genomic Context

Location: 9370..19849
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SM12261_RS00050 (SM12261_0011) tilS 10451..11728 (+) 1278 WP_001209052.1 tRNA lysidine(34) synthetase TilS -
  SM12261_RS00055 (SM12261_0012) hpt 11733..12275 (+) 543 WP_000892173.1 hypoxanthine phosphoribosyltransferase -
  SM12261_RS00060 (SM12261_0013) ftsH 12291..14249 (+) 1959 WP_000744563.1 ATP-dependent zinc metalloprotease FtsH -
  SM12261_RS00065 (SM12261_0014) comX/sigX/comX1/sigX1 14370..14849 (+) 480 WP_000471939.1 sigma-70 family RNA polymerase sigma factor Regulator

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  comX/sigX/comX1/sigX1 late competence genes positive effect
  comX/sigX/comX2/sigX2 late competence genes positive effect
  comX/sigX/comX2/sigX2 late competence genes positive effect
  comE comX/sigX/comX2/sigX2 positive effect
  comW comX/sigX/comX2/sigX2 positive effect
  comX/sigX/comX1/sigX1 late competence genes positive effect
  comW comX/sigX/comX1/sigX1 positive effect
  comE comX/sigX/comX1/sigX1 positive effect
  comE comD positive effect
  comD comE positive effect
  comC comD positive effect
  comE comE positive effect
  comE comB positive effect
  comE comC positive effect
  comE comM positive effect
  comE comW positive effect
  comE comA positive effect
  comB comC positive effect
  comA comC positive effect
  comM cbpD negative effect

Sequence

Protein


Download         Length: 159 a.a.        Molecular weight: 20031.74 Da        Isoelectric Point: 7.4730

>NTDB_id=488 SM12261_RS00065 WP_000471939.1 14370..14849(+) (comX/sigX/comX1/sigX1) [Streptococcus mitis NCTC 12261]
MFKELYEEVQGIVYKCRNEYHLHLWELSDWDQEGMICLHELISREEGIVEDIPRLRKYFKTKFRNRILDYIRKQESQKRR
YDKEPYEEVGEISHRISEGGMWLDEYYLFHETLRDYRNKQSKDKQEELERVLRHERFRGRQRVLRDLRIVFKEFDIRTH

Nucleotide


Download         Length: 480 bp        

>NTDB_id=488 SM12261_RS00065 WP_000471939.1 14370..14849(+) (comX/sigX/comX1/sigX1) [Streptococcus mitis NCTC 12261]
ATGTTTAAAGAATTATATGAAGAAGTCCAGGGGATTGTATATAAGTGTAGAAATGAATATCATCTCCATTTATGGGAGCT
ATCAGATTGGGACCAAGAGGGAATGATTTGCTTACATGAATTGATTAGTAGAGAAGAAGGAATAGTAGAAGATATCCCAC
GCTTACGTAAATACTTCAAAACTAAATTCCGTAATCGGATCCTAGACTATATCCGTAAGCAGGAAAGCCAGAAGCGTAGA
TATGATAAAGAACCCTATGAAGAAGTAGGTGAGATTAGTCATCGTATCAGTGAGGGAGGTATGTGGCTAGATGAGTATTA
TCTCTTTCATGAGACACTAAGAGATTATAGAAACAAACAAAGTAAAGACAAACAAGAAGAGTTAGAACGCGTCTTAAGAC
ATGAACGCTTCCGAGGGCGTCAAAGAGTATTGAGAGACTTACGTATTGTGTTTAAGGAGTTTGATATCCGTACTCATTAG

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comX/sigX/comX2/sigX2 Streptococcus mitis NCTC 12261

97.484

100

0.975
  comX/sigX/comX2/sigX2 Streptococcus mitis SK321

93.082

100

0.931
  comX/comX2 Streptococcus pneumoniae R6

92.453

100

0.925
  comX/comX1 Streptococcus pneumoniae R6

92.453

100

0.925
  comX/comX1 Streptococcus pneumoniae D39

92.453

100

0.925
  comX/comX2 Streptococcus pneumoniae D39

92.453

100

0.925
  comX/comX1 Streptococcus pneumoniae Rx1

92.453

100

0.925
  comX/comX2 Streptococcus pneumoniae Rx1

92.453

100

0.925
  comX/sigX/comX1/sigX1 Streptococcus mitis SK321

93.038

99.371

0.925
  comX/comX1 Streptococcus pneumoniae TIGR4

91.824

100

0.918
  comX/comX2 Streptococcus pneumoniae TIGR4

91.824

100

0.918
  comR/comR1 Streptococcus gordonii str. Challis substr. CH1

48.077

98.113

0.472
  comR/comR2 Streptococcus gordonii str. Challis substr. CH1

48.077

98.113

0.472
  comX/sigX Streptococcus mutans UA159

46.405

96.226

0.447
  comX/sigX Streptococcus suis isolate S10

44.444

98.077

0.436
  comX/sigX Streptococcus suis D9

44.444

98.077

0.436
  comX/sigX Streptococcus suis P1/7

44.444

98.077

0.436
  comX/sigX/comX1/sigX1 Streptococcus pyogenes MGAS315

39.474

95.597

0.377
  comX/sigX/comX2/sigX2 Streptococcus pyogenes MGAS315

39.474

95.597

0.377
  comX/sigX/comX1/sigX1 Streptococcus pyogenes MGAS8232

40.268

93.711

0.377
  comX/sigX/comX2/sigX2 Streptococcus pyogenes JRS4

40.268

93.711

0.377
  comX/sigX/comX2/sigX2 Streptococcus pyogenes MGAS8232

40.268

93.711

0.377
  comX/sigX/comX1/sigX1 Streptococcus pyogenes JRS4

40.268

93.711

0.377

Multiple sequence alignment    



References

[1] G Salvadori et al. (2018) High-resolution profiles of the Streptococcus mitis CSP signaling pathway reveal core and strain-specific regulated genes. BMC Genomics 19(1):453. [PMID: 29898666]