Detailed information    

experimental Experimentally validated

Overview

Name   comX/sigX/comX2/sigX2   Type   Regulator
Locus tag   SPYM18_RS08475 Genome accession   NC_003485
Coordinates   1632938..1633489 (-) Length   183 a.a.
NCBI ID   WP_011018214.1    Uniprot ID   -
Organism   Streptococcus pyogenes MGAS8232     
Function   activate transcription of late competence genes   
Competence regulation

Function

SigX is functional and able to activate sigX-dependent gene expression within the competence regulon.


Genomic Context

Location: 1627938..1638489
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPYM18_RS08450 (spyM18_1962) - 1629304..1630149 (+) 846 WP_002982833.1 mechanosensitive ion channel family protein -
  SPYM18_RS08455 (spyM18_1963) - 1630214..1630774 (-) 561 WP_002995157.1 TIGR01440 family protein -
  SPYM18_RS08460 (spyM18_1964) - 1630788..1631258 (-) 471 WP_002982824.1 ECF transporter S component -
  SPYM18_RS08465 (spyM18_1965) - 1631248..1632012 (-) 765 WP_002982821.1 bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase -
  SPYM18_RS08470 (spyM18_1966) truA 1632002..1632751 (-) 750 WP_002988132.1 tRNA pseudouridine(38-40) synthase TruA -
  SPYM18_RS08475 (spyM18_1967) comX/sigX/comX2/sigX2 1632938..1633489 (-) 552 WP_011018214.1 sigma-70 family RNA polymerase sigma factor Regulator

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  comX/sigX/comX2/sigX2 late competence genes positive effect
  comX/sigX/comX1/sigX1 late competence genes positive effect
  comX/sigX/comX1/sigX1 late competence genes positive effect
  comR comX/sigX/comX1/sigX1 positive effect
  comS comX/sigX/comX1/sigX1 positive effect
  comX/sigX/comX2/sigX2 late competence genes positive effect
  comR comX/sigX/comX2/sigX2 positive effect
  comS comX/sigX/comX2/sigX2 positive effect
  prx comR negative effect

Sequence

Protein


Download         Length: 183 a.a.        Molecular weight: 22281.12 Da        Isoelectric Point: 10.1133

>NTDB_id=450 SPYM18_RS08475 WP_011018214.1 1632938..1633489(-) (comX/sigX/comX2/sigX2) [Streptococcus pyogenes MGAS8232]
MSCCFFVVLFILGIVKKKRRLKMSIETRAAFEKVKPIILKLKRHYYIQLWDRDDWLQEGHIILLQLLERYPELIEEEERL
YRYFKTKFSSYLKDLLRRQESQKRQFHKLAYEEIGEVAHAIPSRGLWLDDYVAYQEVIASLENQLNSQERMQFKALIRGE
RFKGRRALLRKISPYFKEFAQQL

Nucleotide


Download         Length: 552 bp        

>NTDB_id=450 SPYM18_RS08475 WP_011018214.1 1632938..1633489(-) (comX/sigX/comX2/sigX2) [Streptococcus pyogenes MGAS8232]
GTGTCCTGTTGTTTTTTTGTGGTTCTCTTTATACTAGGGATAGTTAAAAAGAAAAGGAGACTCAAAATGTCGATAGAGAC
AAGAGCAGCGTTTGAAAAAGTTAAGCCCATTATTTTGAAATTAAAGCGACACTATTATATTCAATTGTGGGATAGAGATG
ACTGGTTACAAGAAGGACATATTATCTTATTACAGTTACTAGAAAGGTACCCAGAGTTAATTGAGGAAGAAGAGCGTTTA
TATCGCTATTTTAAAACAAAATTTTCATCTTATTTGAAAGATTTATTACGCCGTCAAGAAAGTCAAAAGCGTCAGTTCCA
TAAGTTAGCATATGAAGAGATAGGGGAGGTTGCACATGCCATTCCATCGAGAGGGTTATGGCTAGACGACTATGTGGCTT
ATCAAGAGGTAATAGCTAGCTTAGAGAACCAATTAAATTCGCAAGAGCGTATGCAGTTTAAAGCACTTATCAGGGGCGAA
CGTTTCAAGGGAAGACGTGCTCTACTTAGGAAGATCAGTCCCTATTTTAAGGAATTTGCACAGCAGTTGTAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comX/sigX/comX1/sigX1 Streptococcus pyogenes MGAS8232

100

100

1
  comX/sigX/comX1/sigX1 Streptococcus pyogenes JRS4

99.454

100

0.995
  comX/sigX/comX2/sigX2 Streptococcus pyogenes JRS4

99.454

100

0.995
  comX/sigX/comX2/sigX2 Streptococcus pyogenes MGAS315

98.907

100

0.989
  comX/sigX/comX1/sigX1 Streptococcus pyogenes MGAS315

98.907

100

0.989
  comX/sigX Streptococcus infantarius subsp. infantarius ATCC BAA-102

49.333

94.937

0.468
  comX/sigX Streptococcus mutans UA159

44.444

95.625

0.425
  comX/sigX/comX2/sigX2 Streptococcus mitis SK321

42.953

93.711

0.403
  comX/sigX Streptococcus suis P1/7

40

99.359

0.397
  comX/sigX Streptococcus suis D9

40

99.359

0.397
  comX/sigX Streptococcus suis isolate S10

40

99.359

0.397
  comX/sigX/comX2/sigX2 Streptococcus mitis NCTC 12261

42.282

93.711

0.396
  comX Streptococcus sobrinus strain NIDR 6715-7

46.104

84.153

0.388
  comX Streptococcus thermophilus LMD-9

42.667

90.909

0.388
  comX/sigX Streptococcus salivarius strain HSISS4

42.667

90.909

0.388
  comX Streptococcus thermophilus LMG 18311

42.667

90.909

0.388
  comX Streptococcus salivarius SK126

42.667

90.909

0.388
  comR/comR1 Streptococcus gordonii str. Challis substr. CH1

40.26

96.25

0.388
  comR/comR2 Streptococcus gordonii str. Challis substr. CH1

40.26

96.25

0.388
  comX/sigX/comX1/sigX1 Streptococcus mitis SK321

40.94

93.711

0.384
  comX/comX2 Streptococcus pneumoniae TIGR4

40

94.34

0.377
  comX/comX1 Streptococcus pneumoniae TIGR4

40

94.34

0.377
  comX/comX1 Streptococcus pneumoniae R6

40

94.34

0.377
  comX/comX1 Streptococcus pneumoniae D39

40

94.34

0.377
  comX/comX2 Streptococcus pneumoniae R6

40

94.34

0.377
  comX/comX2 Streptococcus pneumoniae Rx1

40

94.34

0.377
  comX/comX1 Streptococcus pneumoniae Rx1

40

94.34

0.377
  comX/comX2 Streptococcus pneumoniae D39

40

94.34

0.377
  comX/sigX/comX1/sigX1 Streptococcus mitis NCTC 12261

40.268

93.711

0.377

Multiple sequence alignment    



References

[1] Lauren Mashburn-Warren et al. (2012) The cryptic competence pathway in Streptococcus pyogenes is controlled by a peptide pheromone. Journal of Bacteriology 194(17):4589-600. [PMID: 22730123]