Detailed information    

experimental Experimentally validated

Overview

Name   comX/sigX/comX1/sigX1   Type   Regulator
Locus tag   SMSK321_RS09125 Genome accession   NZ_AEDT01000028
Coordinates   29..508 (-) Length   159 a.a.
NCBI ID   WP_000471944.1    Uniprot ID   -
Organism   Streptococcus mitis SK321     
Function   activate transcription of late competence genes   
Competence regulation

Function

Upregulated in response to CSP as measured by RNA-seq.


Genomic Context

Location: 1..5508
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SMSK321_RS09125 (SMSK321_1251) comX/sigX/comX1/sigX1 29..508 (-) 480 WP_000471944.1 hypothetical protein Regulator
  SMSK321_RS09130 (SMSK321_1252) nusG 629..1165 (-) 537 WP_000376740.1 transcription termination/antitermination protein NusG -
  SMSK321_RS09135 (SMSK321_1253) secE 1219..1395 (-) 177 WP_001210990.1 preprotein translocase subunit SecE -
  SMSK321_RS09140 rpmG 1405..1557 (-) 153 WP_001809375.1 50S ribosomal protein L33 -
  SMSK321_RS09145 (SMSK321_1254) pbp2a 1610..3805 (-) 2196 WP_000762646.1 penicillin-binding protein PBP2A -
  SMSK321_RS09150 (SMSK321_1255) - 3892..4767 (+) 876 WP_080550781.1 RluA family pseudouridine synthase -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  comX/sigX/comX1/sigX1 late competence genes positive effect
  comX/sigX/comX1/sigX1 late competence genes positive effect
  comE comX/sigX/comX1/sigX1 positive effect
  comW comX/sigX/comX1/sigX1 positive effect
  comX/sigX/comX2/sigX2 late competence genes positive effect
  comX/sigX/comX2/sigX2 late competence genes positive effect
  comE comX/sigX/comX2/sigX2 positive effect
  comW comX/sigX/comX2/sigX2 positive effect
  comE comE positive effect
  comE comM positive effect
  comE comC positive effect
  comE comD positive effect
  comE comA positive effect
  comE comW positive effect
  comE comB positive effect
  comD comE positive effect
  comM cbpD negative effect
  comC comD positive effect
  comB comC positive effect
  comA comC positive effect

Sequence

Protein


Download         Length: 159 a.a.        Molecular weight: 19990.70 Da        Isoelectric Point: 8.6704

>NTDB_id=516 SMSK321_RS09125 WP_000471944.1 29..508(-) (comX/sigX/comX1/sigX1) [Streptococcus mitis SK321]
MFKELYKEVQGIVYKSRNEYYLHLWELSDWDQEGMICLHELISKEEELAEDIPRLRKYFKTKFRNRILDYIRKQESQKRR
YDKESYEEVGEISHRIGEGGLWLDEYYLFHETLRDYRSKQSKDKQEELERVLRHERFRGRQRVLRDLRIVFKEFDIRTR

Nucleotide


Download         Length: 480 bp        

>NTDB_id=516 SMSK321_RS09125 WP_000471944.1 29..508(-) (comX/sigX/comX1/sigX1) [Streptococcus mitis SK321]
ATGTTTAAAGAATTGTATAAAGAAGTCCAGGGAATTGTATATAAGAGTAGAAATGAATATTACTTGCATTTATGGGAACT
ATCGGATTGGGACCAAGAGGGGATGATTTGCTTGCATGAATTGATTAGTAAAGAAGAAGAGTTGGCAGAAGATATTCCAC
GCTTACGTAAATACTTCAAAACTAAGTTCCGTAATCGAATTCTAGACTATATCCGTAAGCAAGAAAGTCAAAAACGAAGA
TATGATAAAGAATCCTATGAAGAAGTAGGTGAAATCAGTCATCGTATAGGTGAGGGAGGTCTCTGGCTAGATGAGTATTA
TCTCTTTCATGAGACACTAAGAGATTATAGAAGTAAACAAAGTAAAGACAAACAAGAAGAGTTAGAACGGGTCCTAAGAC
ATGAACGCTTCCGAGGGCGTCAAAGAGTATTAAGAGACTTACGTATTGTGTTTAAGGAGTTTGATATCCGTACTCGGTAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comX/sigX/comX2/sigX2 Streptococcus mitis SK321

96.855

100

0.969
  comX/sigX/comX2/sigX2 Streptococcus mitis NCTC 12261

94.304

99.371

0.937
  comX/sigX/comX1/sigX1 Streptococcus mitis NCTC 12261

93.038

99.371

0.925
  comX/comX1 Streptococcus pneumoniae R6

89.241

99.371

0.887
  comX/comX2 Streptococcus pneumoniae R6

89.241

99.371

0.887
  comX/comX2 Streptococcus pneumoniae Rx1

89.241

99.371

0.887
  comX/comX1 Streptococcus pneumoniae Rx1

89.241

99.371

0.887
  comX/comX2 Streptococcus pneumoniae D39

89.241

99.371

0.887
  comX/comX1 Streptococcus pneumoniae D39

89.241

99.371

0.887
  comX/comX2 Streptococcus pneumoniae TIGR4

88.608

99.371

0.881
  comX/comX1 Streptococcus pneumoniae TIGR4

88.608

99.371

0.881
  comR/comR1 Streptococcus gordonii str. Challis substr. CH1

50

98.113

0.491
  comR/comR2 Streptococcus gordonii str. Challis substr. CH1

50

98.113

0.491
  comX/sigX Streptococcus mutans UA159

47.059

96.226

0.453
  comX/sigX Streptococcus suis P1/7

45.098

98.077

0.442
  comX/sigX Streptococcus suis D9

45.098

98.077

0.442
  comX/sigX Streptococcus suis isolate S10

45.098

98.077

0.442
  comX/sigX/comX1/sigX1 Streptococcus pyogenes MGAS315

40.132

95.597

0.384
  comX/sigX/comX2/sigX2 Streptococcus pyogenes MGAS315

40.132

95.597

0.384
  comX/sigX/comX2/sigX2 Streptococcus pyogenes MGAS8232

40.94

93.711

0.384
  comX/sigX/comX1/sigX1 Streptococcus pyogenes MGAS8232

40.94

93.711

0.384
  comX/sigX/comX1/sigX1 Streptococcus pyogenes JRS4

40.94

93.711

0.384
  comX/sigX/comX2/sigX2 Streptococcus pyogenes JRS4

40.94

93.711

0.384
  comX Streptococcus salivarius SK126

37.419

97.484

0.365
  comX Streptococcus thermophilus LMG 18311

37.419

97.484

0.365
  comX Streptococcus thermophilus LMD-9

37.419

97.484

0.365

Multiple sequence alignment    



References

[1] G Salvadori et al. (2018) High-resolution profiles of the Streptococcus mitis CSP signaling pathway reveal core and strain-specific regulated genes. BMC Genomics 19(1):453. [PMID: 29898666]