Detailed information NTG0000447

Experimentally validated

Overview

• Name: comX/sigX/comX1/sigX1
• Type: Regulator
• Locus tag: SpyM6JRS4_RS01370
• Genome accession: NZ_CP011414
• Coordinates: 262663..263214 (+)
• Length: 183 a.a.
• NCBI ID: WP_010921894.1
• Uniprot ID: -
• Organism: Streptococcus pyogenes JRS4
• Function: activate transcription of late competence genes Competence regulation

Function

Expression of sigX from this promoter led to transcription of the sigma(X)-dependent cinA promoter in GAS cells. We found that expression of the sigX gene in a clpP mutant strain resulted in greater accumulation of sigma(X) protein, which resulted in higher levels of transcription from the sigma(X)-dependent promoters cinA, smf, and cglA.

Genomic Context

Location: 257663..268214

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SpyM6JRS4_RS01370 (SpyM6JRS4_01365)	comX/sigX/comX1/sigX1	262663..263214 (+)	552	WP_010921894.1	sigma-70 family RNA polymerase sigma factor	Regulator
SpyM6JRS4_RS01375 (SpyM6JRS4_01370)	-	263773..264300 (+)	528	WP_021340513.1	YqeG family HAD IIIA-type phosphatase	-
SpyM6JRS4_RS01380 (SpyM6JRS4_01375)	yqeH	264300..265418 (+)	1119	WP_011184178.1	ribosome biogenesis GTPase YqeH	-
SpyM6JRS4_RS01385 (SpyM6JRS4_01380)	yhbY	265444..265752 (+)	309	WP_002991094.1	ribosome assembly RNA-binding protein YhbY	-
SpyM6JRS4_RS01390 (SpyM6JRS4_01385)	-	265821..266453 (+)	633	WP_011184179.1	nicotinate-nucleotide adenylyltransferase	-
SpyM6JRS4_RS01395 (SpyM6JRS4_01390)	yqeK	266450..267043 (+)	594	WP_002985989.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
SpyM6JRS4_RS01400 (SpyM6JRS4_01395)	rsfS	267067..267420 (+)	354	WP_011017339.1	ribosome silencing factor	-
SpyM6JRS4_RS01405 (SpyM6JRS4_01400)	-	267468..268211 (+)	744	WP_014407307.1	class I SAM-dependent methyltransferase	-

Regulatory network

Positive effect      

Negative effect

Regulator	Target	Regulation
comX/sigX/comX1/sigX1	late competence genes	positive effect
comX/sigX/comX2/sigX2	late competence genes	positive effect
comX/sigX/comX2/sigX2	late competence genes	positive effect
clpP	comX/sigX/comX2/sigX2	negative effect
comX/sigX/comX1/sigX1	late competence genes	positive effect
clpP	comX/sigX/comX1/sigX1	negative effect

Sequence

Protein

• Length: 183 a.a.
• Molecular weight: 22281.08 Da
• Isoelectric Point: 10.0375

>NTDB_id=447 SpyM6JRS4_RS01370 WP_010921894.1 262663..263214(+) (comX/sigX/comX1/sigX1) [Streptococcus pyogenes JRS4]
MSCCFFVVLFILGIVKKKRRLKMSIETRAAFEKVKPIILKLKRHYYIQLWDRDDWLQEGHIILLQLLERYPELIEEEERL
YRYFKTKFSSYLKDLLRRQESQKRQFHKLAYEEIGEVAHAIPSRGLWLDDYVAYQEVIASLENQLNSQERMQFQALIRGE
RFKGRRALLRKISPYFKEFAQQL

Nucleotide

• Length: 552 bp

>NTDB_id=447 SpyM6JRS4_RS01370 WP_010921894.1 262663..263214(+) (comX/sigX/comX1/sigX1) [Streptococcus pyogenes JRS4]
ATGTCCTGTTGTTTTTTTGTGGTTCTCTTTATACTAGGGATAGTTAAAAAGAAAAGGAGACTCAAAATGTCGATAGAGAC
AAGAGCAGCGTTTGAAAAAGTTAAGCCCATTATTTTGAAATTAAAGCGACACTATTATATTCAATTGTGGGATAGAGATG
ACTGGTTACAAGAAGGACATATTATCTTATTACAGTTACTAGAAAGGTACCCAGAGTTAATTGAGGAAGAAGAGCGTTTA
TATCGCTATTTTAAAACAAAATTTTCATCTTATTTGAAAGATTTATTACGCCGTCAAGAAAGTCAAAAGCGTCAGTTCCA
TAAGTTAGCATATGAAGAGATAGGGGAGGTTGCACATGCCATTCCATCGAGAGGGTTATGGCTAGACGACTATGTGGCTT
ATCAAGAGGTAATAGCTAGCTTAGAGAACCAATTAAATTCGCAAGAGCGTATGCAGTTTCAAGCACTTATCAGGGGCGAA
CGTTTCAAGGGAAGACGTGCTCTACTTAGGAAGATCAGTCCCTATTTTAAGGAATTTGCACAGCAGTTGTAA

Domains

No domain identified.

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comX/sigX/comX2/sigX2	Streptococcus pyogenes JRS4	100	100	1
comX/sigX/comX2/sigX2	Streptococcus pyogenes MGAS8232	99.454	100	0.995
comX/sigX/comX1/sigX1	Streptococcus pyogenes MGAS8232	99.454	100	0.995
comX/sigX/comX2/sigX2	Streptococcus pyogenes MGAS315	99.454	100	0.995
comX/sigX/comX1/sigX1	Streptococcus pyogenes MGAS315	99.454	100	0.995
comX/sigX	Streptococcus infantarius subsp. infantarius ATCC BAA-102	49.333	94.937	0.468
comX/sigX	Streptococcus mutans UA159	44.444	95.625	0.425
comX/sigX/comX2/sigX2	Streptococcus mitis SK321	42.953	93.711	0.403
comX/sigX	Streptococcus suis P1/7	40	99.359	0.397
comX/sigX	Streptococcus suis D9	40	99.359	0.397
comX/sigX	Streptococcus suis isolate S10	40	99.359	0.397
comX/sigX/comX2/sigX2	Streptococcus mitis NCTC 12261	42.282	93.711	0.396
comX	Streptococcus sobrinus strain NIDR 6715-7	46.104	84.153	0.388
comX	Streptococcus thermophilus LMD-9	42.667	90.909	0.388
comX/sigX	Streptococcus salivarius strain HSISS4	42.667	90.909	0.388
comX	Streptococcus thermophilus LMG 18311	42.667	90.909	0.388
comX	Streptococcus salivarius SK126	42.667	90.909	0.388
comR/comR1	Streptococcus gordonii str. Challis substr. CH1	40.26	96.25	0.388
comR/comR2	Streptococcus gordonii str. Challis substr. CH1	40.26	96.25	0.388
comX/sigX/comX1/sigX1	Streptococcus mitis SK321	40.94	93.711	0.384
comX/comX2	Streptococcus pneumoniae TIGR4	40	94.34	0.377
comX/comX1	Streptococcus pneumoniae TIGR4	40	94.34	0.377
comX/comX1	Streptococcus pneumoniae R6	40	94.34	0.377
comX/comX1	Streptococcus pneumoniae D39	40	94.34	0.377
comX/comX2	Streptococcus pneumoniae R6	40	94.34	0.377
comX/comX2	Streptococcus pneumoniae Rx1	40	94.34	0.377
comX/comX1	Streptococcus pneumoniae Rx1	40	94.34	0.377
comX/comX2	Streptococcus pneumoniae D39	40	94.34	0.377
comX/sigX/comX1/sigX1	Streptococcus mitis NCTC 12261	40.268	93.711	0.377

References

• [1]: Robyn L Woodbury et al. (2006) Sigma X induces competence gene expression in Streptococcus pyogenes. Research in Microbiology 157(9):851-6. [PMID: 16963231]
• [2]: Jason A Opdyke et al. (2003) Expression of the secondary sigma factor sigmaX in Streptococcus pyogenes is restricted at two levels. Journal of Bacteriology 185(15):4291-7. [PMID: 12867436]