Detailed information    

experimental Experimentally validated

Overview

Name   comX   Type   Regulator
Locus tag   STRSA0001_RS07545 Genome accession   NZ_ACLO01000087
Coordinates   10584..11081 (-) Length   165 a.a.
NCBI ID   WP_002885610.1    Uniprot ID   A0A2I1TJX9
Organism   Streptococcus salivarius SK126     
Function   activate transcription of late competence genes   
Competence regulation

Function

ComX controls expression of the late competence genes, which are essential for DNA binding, uptake and recombination.


Genomic Context

Location: 5584..16081
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  STRSA0001_RS07525 (STRSA0001_1802) - 5618..7222 (-) 1605 WP_002885608.1 CTP synthase -
  STRSA0001_RS07530 (STRSA0001_1803) rpoE 7475..8050 (-) 576 WP_002885605.1 DNA-directed RNA polymerase subunit delta -
  STRSA0001_RS07535 (STRSA0001_1804) tig 8208..9491 (-) 1284 WP_002885606.1 trigger factor -
  STRSA0001_RS07540 (STRSA0001_1806) - 9634..10476 (+) 843 WP_037605435.1 mechanosensitive ion channel family protein -
  STRSA0001_RS07545 (STRSA0001_1807) comX 10584..11081 (-) 498 WP_002885610.1 sigma factor Regulator

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  comX late competence genes positive effect
  comX late competence genes positive effect
  comR comX positive effect
  comS comX positive effect
  comR comS positive effect
  comS comS positive effect

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 20008.84 Da        Isoelectric Point: 4.9953

>NTDB_id=50 STRSA0001_RS07545 WP_002885610.1 10584..11081(-) (comX) [Streptococcus salivarius SK126]
MEQEVFVHAYEVVRPIVLKASRQYFIQLWDLADMEQEAMMTLYQLLEKFPELKSDDDKLRRYFKTKFRNRLNDEVRRQES
VKRQANRQCYIEISDIAFCLPNKELDAVDRLVYDEQLNAFRNQLSSEDATKLDRLLAGECFRGRKKMIRELKFWMVDFDP
YNEDD

Nucleotide


Download         Length: 498 bp        

>NTDB_id=50 STRSA0001_RS07545 WP_002885610.1 10584..11081(-) (comX) [Streptococcus salivarius SK126]
ATGGAACAAGAAGTTTTTGTTCATGCATATGAAGTTGTAAGGCCGATTGTACTTAAGGCTAGTAGACAATATTTCATCCA
ACTTTGGGACCTTGCTGATATGGAGCAAGAGGCTATGATGACGCTTTATCAGCTTTTAGAAAAGTTTCCTGAATTAAAGT
CTGATGATGATAAGTTACGGCGTTATTTTAAAACTAAATTTAGGAATAGACTTAATGATGAGGTTAGAAGGCAGGAGTCT
GTAAAACGTCAAGCTAATCGACAATGTTATATTGAAATTTCAGATATTGCTTTTTGTCTTCCAAATAAAGAGTTAGATGC
TGTTGATAGGCTTGTTTATGATGAGCAGCTTAATGCTTTCCGTAATCAGTTGTCTTCGGAAGATGCTACTAAGTTAGACC
GTTTGCTAGCTGGGGAGTGTTTTAGAGGTCGAAAAAAGATGATTCGAGAGTTAAAATTTTGGATGGTTGATTTTGATCCT
TACAATGAAGATGATTAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2I1TJX9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comX/sigX Streptococcus salivarius strain HSISS4

99.394

100

0.994
  comX Streptococcus thermophilus LMD-9

88.485

100

0.885
  comX Streptococcus thermophilus LMG 18311

88.485

100

0.885
  comR/comR2 Streptococcus gordonii str. Challis substr. CH1

43.226

96.875

0.419
  comR/comR1 Streptococcus gordonii str. Challis substr. CH1

43.226

96.875

0.419
  comX/sigX Streptococcus mutans UA159

41.667

97.5

0.406
  comX Streptococcus sobrinus strain NIDR 6715-7

40.606

100

0.406
  comX/sigX Streptococcus infantarius subsp. infantarius ATCC BAA-102

41.026

98.734

0.405
  comX/sigX Streptococcus suis P1/7

39.869

98.077

0.391
  comX/sigX Streptococcus suis D9

39.869

98.077

0.391
  comX/sigX Streptococcus suis isolate S10

39.869

98.077

0.391
  comX/sigX/comX2/sigX2 Streptococcus pyogenes MGAS8232

42.667

90.909

0.388
  comX/sigX/comX1/sigX1 Streptococcus pyogenes MGAS8232

42.667

90.909

0.388
  comX/sigX/comX2/sigX2 Streptococcus pyogenes JRS4

42.667

90.909

0.388
  comX/sigX/comX1/sigX1 Streptococcus pyogenes JRS4

42.667

90.909

0.388
  comX/sigX/comX2/sigX2 Streptococcus pyogenes MGAS315

42

90.909

0.382
  comX/sigX/comX1/sigX1 Streptococcus pyogenes MGAS315

42

90.909

0.382
  comX/comX2 Streptococcus pneumoniae TIGR4

38.926

93.711

0.365
  comX/comX1 Streptococcus pneumoniae TIGR4

38.926

93.711

0.365
  comX/sigX/comX1/sigX1 Streptococcus mitis SK321

37.419

97.484

0.365
  comX/sigX/comX2/sigX2 Streptococcus mitis SK321

37.419

97.484

0.365
  comX/sigX/comX2/sigX2 Streptococcus mitis NCTC 12261

37.419

97.484

0.365

Multiple sequence alignment    



References

[1] Laetitia Fontaine et al. (2010) A novel pheromone quorum-sensing system controls the development of natural competence in Streptococcus thermophilus and Streptococcus salivarius. Journal of Bacteriology 192(5):1444-54. [PMID: 20023010]