Detailed information    

experimental Experimentally validated

Overview

Name   comX/sigX/comX2/sigX2   Type   Regulator
Locus tag   SMSK321_RS10700 Genome accession   NZ_AEDT01000032
Coordinates   252802..253281 (+) Length   159 a.a.
NCBI ID   WP_000471945.1    Uniprot ID   -
Organism   Streptococcus mitis SK321     
Function   activate transcription of late competence genes   
Competence regulation

Function

Upregulated in response to CSP as measured by RNA-seq.


Genomic Context

Location: 247802..258281
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SMSK321_RS10685 (SMSK321_1648) tilS 248883..250160 (+) 1278 WP_001209051.1 tRNA lysidine(34) synthetase TilS -
  SMSK321_RS10690 (SMSK321_1649) hpt 250165..250707 (+) 543 WP_000892178.1 hypoxanthine phosphoribosyltransferase -
  SMSK321_RS10695 (SMSK321_1650) ftsH 250723..252681 (+) 1959 WP_000744564.1 ATP-dependent zinc metalloprotease FtsH -
  SMSK321_RS10700 (SMSK321_1651) comX/sigX/comX2/sigX2 252802..253281 (+) 480 WP_000471945.1 hypothetical protein Regulator

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  comX/sigX/comX2/sigX2 late competence genes positive effect
  comX/sigX/comX1/sigX1 late competence genes positive effect
  comX/sigX/comX1/sigX1 late competence genes positive effect
  comE comX/sigX/comX1/sigX1 positive effect
  comW comX/sigX/comX1/sigX1 positive effect
  comX/sigX/comX2/sigX2 late competence genes positive effect
  comE comX/sigX/comX2/sigX2 positive effect
  comW comX/sigX/comX2/sigX2 positive effect
  comE comE positive effect
  comE comM positive effect
  comE comC positive effect
  comE comD positive effect
  comE comA positive effect
  comE comW positive effect
  comE comB positive effect
  comD comE positive effect
  comM cbpD negative effect
  comC comD positive effect
  comB comC positive effect
  comA comC positive effect

Sequence

Protein


Download         Length: 159 a.a.        Molecular weight: 19988.68 Da        Isoelectric Point: 8.0341

>NTDB_id=517 SMSK321_RS10700 WP_000471945.1 252802..253281(+) (comX/sigX/comX2/sigX2) [Streptococcus mitis SK321]
MFKELYKEVQGIVYKSRNEYYLHLWELSDWDQEGMICLHELISREEELVEDIPRLRKYFKTKFRNRILDYIRKQESQKRR
YDKEAYEEVGEISHRIGEGGLWLDDYYLFHETLRDYRSKQSKDKQEELERVLRHERFRGRQRVLRDLRIVFKEFDIRTQ

Nucleotide


Download         Length: 480 bp        

>NTDB_id=517 SMSK321_RS10700 WP_000471945.1 252802..253281(+) (comX/sigX/comX2/sigX2) [Streptococcus mitis SK321]
ATGTTTAAAGAATTGTATAAAGAAGTCCAGGGAATTGTATATAAGAGTAGAAATGAATATTACTTGCATTTATGGGAACT
ATCGGATTGGGACCAAGAGGGAATGATTTGCTTACATGAATTGATCAGTAGAGAAGAAGAGCTAGTAGAAGATATTCCTC
GTTTAAGGAAATATTTTAAGACTAAGTTCCGGAATCGAATCCTAGACTATATCCGTAAGCAGGAAAGTCAAAAACGAAGA
TATGATAAAGAAGCCTATGAAGAAGTAGGTGAGATCAGTCATCGTATAGGTGAGGGAGGTCTGTGGCTAGATGACTATTA
TCTCTTTCATGAGACACTAAGAGATTATAGAAGCAAACAAAGTAAAGACAAACAAGAAGAGTTAGAACGGGTCCTAAGAC
ATGAACGCTTCCGAGGTCGTCAAAGAGTATTAAGAGACTTACGTATTGTGTTTAAAGAGTTTGATATCCGTACTCAATAG

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comX/sigX/comX1/sigX1 Streptococcus mitis SK321

96.855

100

0.969
  comX/sigX/comX2/sigX2 Streptococcus mitis NCTC 12261

94.34

100

0.943
  comX/sigX/comX1/sigX1 Streptococcus mitis NCTC 12261

93.082

100

0.931
  comX/comX2 Streptococcus pneumoniae R6

90.566

100

0.906
  comX/comX2 Streptococcus pneumoniae D39

90.566

100

0.906
  comX/comX1 Streptococcus pneumoniae D39

90.566

100

0.906
  comX/comX2 Streptococcus pneumoniae Rx1

90.566

100

0.906
  comX/comX1 Streptococcus pneumoniae Rx1

90.566

100

0.906
  comX/comX1 Streptococcus pneumoniae R6

90.566

100

0.906
  comX/comX2 Streptococcus pneumoniae TIGR4

89.937

100

0.899
  comX/comX1 Streptococcus pneumoniae TIGR4

89.937

100

0.899
  comR/comR2 Streptococcus gordonii str. Challis substr. CH1

49.359

98.113

0.484
  comR/comR1 Streptococcus gordonii str. Challis substr. CH1

49.359

98.113

0.484
  comX/sigX Streptococcus suis P1/7

45.098

98.077

0.442
  comX/sigX Streptococcus suis D9

45.098

98.077

0.442
  comX/sigX Streptococcus suis isolate S10

45.098

98.077

0.442
  comX/sigX Streptococcus mutans UA159

45.752

96.226

0.44
  comX/sigX/comX2/sigX2 Streptococcus pyogenes MGAS8232

42.953

93.711

0.403
  comX/sigX/comX2/sigX2 Streptococcus pyogenes JRS4

42.953

93.711

0.403
  comX/sigX/comX1/sigX1 Streptococcus pyogenes MGAS8232

42.953

93.711

0.403
  comX/sigX/comX1/sigX1 Streptococcus pyogenes JRS4

42.953

93.711

0.403
  comX/sigX/comX2/sigX2 Streptococcus pyogenes MGAS315

42.105

95.597

0.403
  comX/sigX/comX1/sigX1 Streptococcus pyogenes MGAS315

42.105

95.597

0.403
  comX Streptococcus sobrinus strain NIDR 6715-7

37.662

96.855

0.365
  comX Streptococcus thermophilus LMD-9

37.419

97.484

0.365
  comX Streptococcus thermophilus LMG 18311

37.419

97.484

0.365
  comX Streptococcus salivarius SK126

37.419

97.484

0.365

Multiple sequence alignment    



References

[1] G Salvadori et al. (2018) High-resolution profiles of the Streptococcus mitis CSP signaling pathway reveal core and strain-specific regulated genes. BMC Genomics 19(1):453. [PMID: 29898666]