Detailed information NTG0000424

Experimentally validated

Overview

• Name: comR/comR2
• Type: Regulator
• Locus tag: SGO_RS10430
• Genome accession: NC_009785
• Coordinates: 2178847..2179329 (-)
• Length: 160 a.a.
• NCBI ID: WP_012130755.1
• Uniprot ID: Q6XLQ2
• Organism: Streptococcus gordonii str. Challis substr. CH1
• Function: activate transcription of late competence genes Competence regulation

Function

Like the identical twin comX loci of S. pneumoniae, both comR determinants are independently functional as well as responsive to the ComDE signal transduction system activated by competence-stimulating peptide.

Genomic Context

Location: 2173847..2184329

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SGO_RS10430 (SGO_2130)	comR/comR2	2178847..2179329 (-)	483	WP_012130755.1	sigma-70 family RNA polymerase sigma factor	Regulator
SGO_RS10445 (SGO_2133)	ftsH	2179860..2181842 (-)	1983	WP_012131068.1	ATP-dependent zinc metalloprotease FtsH	-
SGO_RS10450 (SGO_2134)	hpt	2181861..2182403 (-)	543	WP_012131069.1	hypoxanthine phosphoribosyltransferase	-
SGO_RS10455 (SGO_2135)	tilS	2182408..2183685 (-)	1278	WP_012131070.1	tRNA lysidine(34) synthetase TilS	-

Regulatory network

Positive effect      

Negative effect

Regulator	Target	Regulation
comR/comR2	late competence genes	positive effect
comR/comR2	late competence genes	positive effect
comE/comE1	comR/comR2	positive effect
comR/comR1	late competence genes	positive effect
comR/comR1	late competence genes	positive effect
comE/comE1	comR/comR1	positive effect
comE/comE1	comA	positive effect
comA	comC/comC1	positive effect
comE/comE1	comB	positive effect
comB	comC/comC1	positive effect
comE/comE1	comE/comE1	positive effect
comE/comE1	comC/comC1	positive effect
comE/comE1	comD/comD1	positive effect
comD/comD1	comE/comE1	positive effect
comC/comC1	comD/comD1	positive effect

Sequence

Protein

• Length: 160 a.a.
• Molecular weight: 19950.92 Da
• Isoelectric Point: 9.6140

>NTDB_id=424 SGO_RS10430 WP_012130755.1 2178847..2179329(-) (comR/comR2) [Streptococcus gordonii str. Challis substr. CH1]
MKFEDVYKQVEGIVKRCYKDYYLHLWEYADWRQEGMLVLYELLKSHPNLLEDQPRLYRYFKTKFRNRIHDLIRRQESQKR
KLDRQPYEEVSEIGHRLRMKELYLDELVAFRSAMSEYRSRLSPEEAKQYERLMADERFKGRKAMLKDLSYHLRDFNPRLD

Nucleotide

• Length: 483 bp

>NTDB_id=424 SGO_RS10430 WP_012130755.1 2178847..2179329(-) (comR/comR2) [Streptococcus gordonii str. Challis substr. CH1]
ATGAAGTTTGAGGACGTATACAAACAAGTTGAAGGGATAGTCAAGCGATGTTATAAAGATTATTATCTGCACTTATGGGA
GTATGCAGATTGGAGGCAGGAAGGGATGTTGGTGTTGTACGAATTGTTAAAAAGTCACCCCAACCTACTAGAAGATCAAC
CACGACTGTACCGTTACTTCAAGACCAAGTTTCGGAACAGAATTCATGATTTGATTCGTCGCCAAGAGAGTCAAAAACGG
AAATTGGACAGGCAACCCTATGAAGAGGTCAGCGAGATAGGTCATCGTTTAAGAATGAAAGAGCTTTATCTAGATGAACT
GGTAGCCTTTAGAAGTGCCATGTCAGAGTATAGAAGCCGTCTCAGCCCAGAAGAGGCAAAACAATATGAGCGTCTGATGG
CAGATGAAAGATTCAAAGGACGTAAGGCAATGCTCAAAGATCTGTCTTATCACTTAAGGGATTTCAATCCCCGTTTAGAT
TAA

Domains

No domain identified.

3D structure

• AlphaFold DB: Q6XLQ2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comR/comR1	Streptococcus gordonii str. Challis substr. CH1	100	100	1
comX/sigX/comX1/sigX1	Streptococcus mitis SK321	50	98.113	0.491
comX/sigX	Streptococcus suis P1/7	49.032	99.359	0.487
comX/sigX	Streptococcus suis D9	49.032	99.359	0.487
comX/sigX	Streptococcus suis isolate S10	49.032	99.359	0.487
comX/sigX/comX2/sigX2	Streptococcus mitis NCTC 12261	49.359	98.113	0.484
comX/sigX/comX2/sigX2	Streptococcus mitis SK321	49.359	98.113	0.484
comX/comX1	Streptococcus pneumoniae Rx1	48.718	98.113	0.478
comX/comX2	Streptococcus pneumoniae Rx1	48.718	98.113	0.478
comX/comX2	Streptococcus pneumoniae TIGR4	48.718	98.113	0.478
comX/comX1	Streptococcus pneumoniae TIGR4	48.718	98.113	0.478
comX/comX1	Streptococcus pneumoniae D39	48.718	98.113	0.478
comX/comX1	Streptococcus pneumoniae R6	48.718	98.113	0.478
comX/comX2	Streptococcus pneumoniae D39	48.718	98.113	0.478
comX/comX2	Streptococcus pneumoniae R6	48.718	98.113	0.478
comX/sigX/comX1/sigX1	Streptococcus mitis NCTC 12261	48.077	98.113	0.472
comX/sigX	Streptococcus mutans UA159	47.436	97.5	0.463
comX/sigX	Streptococcus salivarius strain HSISS4	43.226	96.875	0.419
comX	Streptococcus salivarius SK126	43.226	96.875	0.419
comX	Streptococcus thermophilus LMD-9	42.581	96.875	0.413
comX	Streptococcus thermophilus LMG 18311	42.581	96.875	0.413
comX	Streptococcus sobrinus strain NIDR 6715-7	42.208	96.25	0.406
comX/sigX	Streptococcus infantarius subsp. infantarius ATCC BAA-102	39.873	100	0.399
comX/sigX/comX2/sigX2	Streptococcus pyogenes MGAS315	40.909	96.25	0.394
comX/sigX/comX1/sigX1	Streptococcus pyogenes MGAS315	40.909	96.25	0.394
comX/sigX/comX2/sigX2	Streptococcus pyogenes MGAS8232	40.26	96.25	0.388
comX/sigX/comX1/sigX1	Streptococcus pyogenes MGAS8232	40.26	96.25	0.388
comX/sigX/comX1/sigX1	Streptococcus pyogenes JRS4	40.26	96.25	0.388
comX/sigX/comX2/sigX2	Streptococcus pyogenes JRS4	40.26	96.25	0.388

References

• [1]: Nicholas C K Heng et al. (2007) Competence-dependent bacteriocin production by Streptococcus gordonii DL1 (Challis). Journal of Bacteriology 189(4):1468-72. [PMID: 17012395]
• [2]: M M Vickerman et al. (2007) Genome-wide transcriptional changes in Streptococcus gordonii in response to competence signaling peptide. Journal of Bacteriology 189(21):7799-807. [PMID: 17720781]
• [3]: Nicholas C K Heng et al. (2006) Identification and characterization of the loci encoding the competence-associated alternative sigma factor of Streptococcus gordonii. FEMS Microbiology Letters 259(1):27-34. [PMID: 16684098]