Detailed information    

experimental Experimentally validated

Overview

Name   comA   Type   Regulator
Locus tag   SGO_RS10260 Genome accession   NC_009785
Coordinates   2159132..2161285 (-) Length   717 a.a.
NCBI ID   WP_012131060.1    Uniprot ID   A8AZY5
Organism   Streptococcus gordonii str. Challis substr. CH1     
Function   processing and transport of ComC   
Competence regulation

Function

In S. gordonii, pre-CSP is a 50 aa polypeptide that is cleaved at a double glycine to generate the mature 19 aa residue CSP. This peptide is then exported out of the cell via an ABC-binding cassette transporter encoded by comA and comB. Once a critical concentration of environmental CSP is reached, it is detected by the two-component system ComDE, comprising a membrane-bound histidine kinase (ComD), which phosphorylates the intracellular response regulator ComE. ComE activates a signalling cascade that includes upregulation of the master regulator SigX (designated ComR in S. gordonii), an alternate sigma factor that controls expression of late competence genes encoding DNA uptake and recombination machinery.


Genomic Context

Location: 2154132..2166285
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SGO_RS10245 (SGO_2092) purD 2154492..2155751 (-) 1260 WP_012131055.1 phosphoribosylamine--glycine ligase -
  SGO_RS10250 (SGO_2094) lytF 2155996..2157645 (+) 1650 WP_012131057.1 GBS Bsp-like repeat-containing protein Regulator
  SGO_RS10255 (SGO_2096) comB 2157764..2159122 (-) 1359 WP_012131059.1 competence pheromone export protein ComB Regulator
  SGO_RS10260 (SGO_2097) comA 2159132..2161285 (-) 2154 WP_012131060.1 peptide cleavage/export ABC transporter ComA Regulator
  SGO_RS10265 (SGO_2098) rpsD 2161739..2162350 (-) 612 WP_008810013.1 30S ribosomal protein S4 -
  SGO_RS10270 (SGO_2099) - 2162616..2163338 (-) 723 WP_008810014.1 ABC transporter ATP-binding protein -
  SGO_RS10275 (SGO_2100) - 2163338..2164336 (-) 999 WP_012131061.1 ABC transporter substrate-binding protein -
  SGO_RS10280 (SGO_2101) - 2164380..2165132 (-) 753 WP_041789832.1 ABC transporter permease -
  SGO_RS10285 (SGO_2102) - 2165095..2165385 (-) 291 WP_012131063.1 thiamine-binding protein -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  comA comC/comC1 positive effect
  comC/comC1 comD/comD1 positive effect
  comD/comD1 comE/comE1 positive effect
  comE/comE1 comA positive effect
  comE/comE1 comD/comD1 positive effect
  comE/comE1 comC/comC1 positive effect
  comE/comE1 comB positive effect
  comE/comE1 comR/comR2 positive effect
  comE/comE1 comE/comE1 positive effect
  comE/comE1 comR/comR1 positive effect
  comB comC/comC1 positive effect
  comR/comR2 late competence genes positive effect
  comR/comR1 late competence genes positive effect
  comR/comR2 late competence genes positive effect
  comR/comR1 late competence genes positive effect

Sequence

Protein


Download         Length: 717 a.a.        Molecular weight: 80740.81 Da        Isoelectric Point: 7.2115

>NTDB_id=421 SGO_RS10260 WP_012131060.1 2159132..2161285(-) (comA) [Streptococcus gordonii str. Challis substr. CH1]
MKFRKQHYRAQVDTRDCGVAALAMIFGYYGSYFSLATLREKAKTTNDGTTALGLVKVAEGLNFETRAFKADMSLFDLEEV
SYPFIAHILKDGKLLHYYVVTGQDKHTIHIADPDPQVKMTKISRERFEQEWTGITIFLAPSPAYKPSQEKKNGLLDFIPL
LIKQKGLITNIVLATLLVTLINIVGSYYLQSIIDTYVPDHMKTTLGMISIGLIIVYILQQFLSYAQEYLLLVLGQRLSID
VILSYIKHVFQLPMSFFATRRTGEIVSRFTDANRIIDALASTILSIFLDVSIVSIIAIVLFSQNSSLFFLTLLGIPVYAL
IIFLFMKPFEKMNHETMEANSLLSSSIIEDINGIETIKSLTSEKQRYQKIDKEFVTYLKKSFAYGRSESLQKVLKAAARL
ILNVLILWLGATLVMDQKISLGQLITYNTLLVYFTNPLENIINLQTKLQSARVANERLNEVYLVKSEFEEKKLIKDLSHF
QADIDFRGVSYKYGYGANVLSEIDLHIPAGSKTSFVGVSGSGKTTLAKMMVHFYAPNQGDICLGGVNLNQLDKQALRQYI
NYLPQQPYVFNGTILENLLLGAREGTTQEDILRAVELAEIRSDIERMPLNYQTELSADGAGISGGQRQRIALARALLTDA
PVLILDEATSSLDILTEKRIIDNLMVLDKTIIFIAHRLTIAERSEQVVVLDQGRIVESGSHKELIEREGFYHHLVNS

Nucleotide


Download         Length: 2154 bp        

>NTDB_id=421 SGO_RS10260 WP_012131060.1 2159132..2161285(-) (comA) [Streptococcus gordonii str. Challis substr. CH1]
ATGAAATTTAGGAAACAGCATTACAGAGCACAAGTTGACACTAGGGATTGCGGTGTTGCAGCTCTGGCTATGATTTTTGG
TTATTATGGATCGTATTTTTCCTTGGCTACACTACGAGAAAAGGCAAAAACGACTAATGATGGGACAACTGCTTTGGGTT
TGGTAAAAGTTGCTGAAGGACTGAACTTTGAGACGCGAGCTTTTAAAGCAGATATGAGCTTATTTGATCTGGAAGAGGTC
TCTTATCCTTTTATAGCGCATATCCTTAAGGATGGGAAGCTTCTGCATTATTATGTTGTAACGGGACAAGATAAACACAC
GATTCATATTGCAGACCCAGATCCTCAAGTTAAGATGACAAAGATTTCGCGAGAGCGTTTCGAACAGGAGTGGACAGGAA
TTACCATTTTCCTGGCTCCTAGTCCGGCCTATAAGCCTAGTCAGGAAAAGAAAAATGGGCTTTTAGATTTCATTCCCTTA
TTGATTAAACAGAAGGGATTGATTACTAATATTGTGCTTGCAACTTTGCTGGTAACGTTGATCAATATTGTTGGATCCTA
CTACCTTCAATCGATTATTGATACTTATGTGCCTGATCATATGAAAACGACGCTGGGTATGATTTCTATCGGCTTGATTA
TTGTCTATATTTTGCAGCAATTTTTGTCTTACGCCCAAGAATATTTGCTTTTGGTTTTAGGGCAGCGCCTATCGATTGAT
GTGATCTTATCCTATATCAAGCACGTTTTTCAGCTACCTATGTCATTTTTTGCGACAAGGCGGACAGGGGAAATCGTCTC
TCGTTTTACGGACGCCAATCGGATTATTGATGCCTTGGCCAGTACAATCTTGTCGATTTTCTTGGATGTGTCAATTGTGT
CGATCATTGCGATTGTTCTTTTTTCACAAAATAGCAGTCTTTTCTTTTTGACCTTGTTAGGAATCCCAGTTTACGCTCTC
ATTATTTTTCTTTTTATGAAGCCTTTTGAAAAGATGAATCATGAGACGATGGAAGCAAATAGTCTGTTGTCGTCTTCCAT
CATTGAAGATATCAATGGGATTGAAACGATCAAGTCTTTGACTAGCGAAAAACAACGTTATCAGAAGATTGACAAGGAAT
TTGTGACTTATCTGAAAAAGTCTTTTGCCTACGGGCGTTCTGAGAGTCTACAAAAAGTCTTGAAAGCGGCTGCTCGTTTG
ATTTTAAACGTCTTGATTTTATGGCTAGGAGCTACACTTGTCATGGATCAAAAAATCAGTCTTGGGCAGCTCATTACTTA
TAATACGCTTCTAGTCTACTTTACTAATCCTTTAGAAAATATTATCAATCTGCAAACGAAGTTACAGTCCGCACGAGTGG
CCAATGAGCGCCTCAATGAGGTCTATTTAGTTAAGTCTGAATTTGAAGAAAAGAAGCTTATTAAAGACTTGAGCCACTTT
CAGGCTGACATCGATTTTCGTGGAGTCAGTTATAAGTATGGCTATGGGGCCAATGTATTGTCAGAGATTGACTTGCATAT
TCCTGCTGGAAGTAAGACCAGCTTTGTTGGTGTATCCGGTTCAGGAAAAACAACCTTAGCCAAGATGATGGTGCATTTCT
ACGCACCAAATCAAGGAGATATTTGTCTGGGAGGAGTCAATCTCAATCAGTTAGATAAGCAGGCACTGCGCCAATATATC
AACTATCTTCCCCAGCAACCGTATGTTTTTAATGGAACTATTTTGGAAAATTTACTCTTAGGTGCGCGTGAAGGTACGAC
TCAAGAAGATATTCTGCGCGCGGTAGAGTTGGCGGAAATTAGGTCGGATATTGAGCGGATGCCCCTCAATTATCAGACAG
AGTTGTCTGCAGATGGTGCAGGAATCTCGGGTGGTCAACGTCAGCGTATTGCTCTAGCAAGGGCGCTCTTGACAGATGCG
CCAGTGCTTATCTTAGATGAAGCAACGAGTAGTCTGGATATTCTAACAGAGAAGCGGATCATTGATAATTTGATGGTGCT
TGATAAGACAATTATCTTTATTGCGCATCGTTTGACTATTGCGGAACGTTCGGAGCAGGTGGTCGTTTTGGATCAAGGGA
GAATTGTCGAGAGTGGAAGTCATAAGGAGCTGATCGAAAGAGAAGGCTTTTACCACCATTTGGTCAATAGTTAG

Domains

Predicted by InterproScan.

Peptidase C39 family (Peptidase_C39)

(7-142)

ABC transporter transmembrane region (ABC_membrane)

(170-438)

ABC transporter (ABC_tran)

(500-650)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A8AZY5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comA Streptococcus mitis NCTC 12261

80.753

100

0.808
  comA Streptococcus pneumoniae R6

80.614

100

0.806
  comA Streptococcus pneumoniae D39

80.614

100

0.806
  comA Streptococcus pneumoniae Rx1

80.614

100

0.806
  comA Streptococcus mitis SK321

80.335

100

0.803
  comA Streptococcus pneumoniae TIGR4

80.195

100

0.802
  comA/nlmT Streptococcus mutans UA159

64.435

100

0.644

Multiple sequence alignment    



References

[1] Nicholas C K Heng et al. (2007) Competence-dependent bacteriocin production by Streptococcus gordonii DL1 (Challis). Journal of Bacteriology 189(4):1468-72. [PMID: 17012395]