Detailed information NTG0000422

Experimentally validated

Overview

• Name: comB
• Type: Regulator
• Locus tag: SGO_RS10255
• Genome accession: NC_009785
• Coordinates: 2157764..2159122 (-)
• Length: 452 a.a.
• NCBI ID: WP_012131059.1
• Uniprot ID: P95761
• Organism: Streptococcus gordonii str. Challis substr. CH1
• Function: transport of ComC Competence regulation

Function

In S. gordonii, pre-CSP is a 50 aa polypeptide that is cleaved at a double glycine to generate the mature 19 aa residue CSP. This peptide is then exported out of the cell via an ABC-binding cassette transporter encoded by comA and comB. Once a critical concentration of environmental CSP is reached, it is detected by the two-component system ComDE, comprising a membrane-bound histidine kinase (ComD), which phosphorylates the intracellular response regulator ComE. ComE activates a signalling cascade that includes upregulation of the master regulator SigX (designated ComR in S. gordonii), an alternate sigma factor that controls expression of late competence genes encoding DNA uptake and recombination machinery.

Genomic Context

Location: 2152764..2164122

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SGO_RS10240 (SGO_2091)	-	2153239..2154432 (-)	1194	WP_012131054.1	ATP-grasp domain-containing protein	-
SGO_RS10245 (SGO_2092)	purD	2154492..2155751 (-)	1260	WP_012131055.1	phosphoribosylamine--glycine ligase	-
SGO_RS10250 (SGO_2094)	lytF	2155996..2157645 (+)	1650	WP_012131057.1	GBS Bsp-like repeat-containing protein	Regulator
SGO_RS10255 (SGO_2096)	comB	2157764..2159122 (-)	1359	WP_012131059.1	competence pheromone export protein ComB	Regulator
SGO_RS10260 (SGO_2097)	comA	2159132..2161285 (-)	2154	WP_012131060.1	peptide cleavage/export ABC transporter ComA	Regulator
SGO_RS10265 (SGO_2098)	rpsD	2161739..2162350 (-)	612	WP_008810013.1	30S ribosomal protein S4	-
SGO_RS10270 (SGO_2099)	-	2162616..2163338 (-)	723	WP_008810014.1	ABC transporter ATP-binding protein	-

Regulatory network

Positive effect      

Negative effect

Regulator	Target	Regulation
comB	comC/comC1	positive effect
comC/comC1	comD/comD1	positive effect
comD/comD1	comE/comE1	positive effect
comE/comE1	comA	positive effect
comA	comC/comC1	positive effect
comE/comE1	comD/comD1	positive effect
comE/comE1	comC/comC1	positive effect
comE/comE1	comB	positive effect
comE/comE1	comR/comR2	positive effect
comE/comE1	comE/comE1	positive effect
comE/comE1	comR/comR1	positive effect
comR/comR2	late competence genes	positive effect
comR/comR1	late competence genes	positive effect
comR/comR2	late competence genes	positive effect
comR/comR1	late competence genes	positive effect

Sequence

Protein

• Length: 452 a.a.
• Molecular weight: 49879.14 Da
• Isoelectric Point: 6.0163

>NTDB_id=422 SGO_RS10255 WP_012131059.1 2157764..2159122(-) (comB) [Streptococcus gordonii str. Challis substr. CH1]
MNEQFLESAEFYQKRYHNFASCLIVPSLILLVFLVGFSMLAKKEITISSRASVEASRVLAQIQSTSNQPIIANHLAENKE
VKKGELLIQYAVEGEGAQEQKFSSQLDLLKDQKGKLETLRSSLESGRNQFTEPDSYGYEQSFKDYQNQVESMTSSVNQQN
ATIASQNAAASQSQAELGGVISDVDSKLNDHRNLKNAIQSGVGIDASHPLHSLYQSYRDQLSLAEDKATAQSQIVAQLDG
QISQLEATAATYRVQYAGAGAQQAYASNLSSQLASLKAQYLVKVGQELTTLTQQILEAESNLKLQETVSKRGQILAEMDG
LLHLNPEVQGSTLVAEGTALAQIYPKITNERKIKIVTYVSSKDVSTIKNGDKVRFITADDANKQMILTSQISSIDANATQ
TKQGNFFKVECEMAVSKDQAKKLRYGLEGKFVMVTGEKTYFSYYMEKFFNLG

Nucleotide

• Length: 1359 bp

>NTDB_id=422 SGO_RS10255 WP_012131059.1 2157764..2159122(-) (comB) [Streptococcus gordonii str. Challis substr. CH1]
ATGAATGAACAATTTTTAGAAAGTGCAGAGTTTTATCAGAAACGTTATCATAATTTTGCTAGTTGTTTAATTGTGCCAAG
TCTTATTTTACTAGTATTTCTAGTTGGTTTTTCCATGCTGGCTAAAAAAGAAATTACGATTTCTAGCCGTGCTTCTGTAG
AAGCTAGCCGAGTGCTAGCTCAGATTCAGTCGACTAGTAACCAGCCTATCATTGCGAATCATTTGGCAGAAAACAAGGAG
GTCAAAAAAGGGGAGTTGCTAATCCAGTATGCAGTAGAAGGAGAGGGAGCTCAGGAACAGAAGTTTTCCAGCCAGCTTGA
CTTACTCAAAGATCAAAAGGGCAAACTAGAAACTTTGCGTTCCAGTTTAGAAAGCGGGCGTAATCAATTTACTGAGCCAG
ATAGTTATGGCTACGAACAAAGCTTTAAAGACTACCAAAATCAAGTAGAGAGTATGACTAGTTCTGTGAATCAGCAAAAT
GCTACGATTGCTTCACAAAATGCAGCGGCCAGTCAGTCTCAGGCGGAACTGGGCGGTGTTATCAGTGATGTGGATAGCAA
ACTAAATGACCATCGAAACTTGAAAAATGCCATTCAAAGTGGGGTGGGTATAGATGCATCGCACCCTCTTCACTCCTTGT
ACCAGTCCTATCGTGATCAGTTGAGCCTGGCGGAGGATAAGGCAACAGCTCAAAGTCAGATTGTAGCTCAGTTAGATGGG
CAAATCTCTCAATTAGAAGCAACAGCTGCGACTTATCGTGTCCAATATGCTGGTGCAGGCGCTCAACAAGCCTATGCTAG
CAATTTATCCAGTCAGTTGGCTTCACTGAAAGCCCAGTATCTGGTCAAGGTAGGACAAGAATTGACCACTCTGACTCAGC
AAATCTTGGAAGCCGAGAGCAATCTTAAACTTCAAGAAACAGTCTCTAAGCGAGGTCAGATTTTGGCTGAAATGGATGGC
TTGCTTCACTTAAATCCAGAAGTACAAGGATCTACTCTGGTGGCAGAAGGAACTGCTTTGGCTCAGATTTACCCCAAAAT
CACTAATGAAAGAAAAATCAAAATTGTCACATATGTTTCTTCCAAGGATGTATCTACTATTAAAAATGGAGACAAGGTGC
GCTTTATCACAGCTGATGATGCTAACAAACAAATGATTTTGACATCCCAAATTTCCAGTATTGATGCTAATGCTACCCAA
ACCAAACAGGGAAATTTCTTTAAAGTAGAATGCGAAATGGCTGTTAGTAAAGATCAAGCTAAGAAGCTCCGCTATGGCTT
GGAAGGTAAATTTGTCATGGTAACTGGGGAAAAGACTTATTTCAGCTATTATATGGAAAAATTTTTCAATCTTGGTTGA

Domains

Predicted by InterproScan.

• HlyD family secretion protein (HlyD_3) (314-426)

3D structure

• AlphaFold DB: P95761

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comB	Streptococcus mitis NCTC 12261	55.778	100	0.559
comB	Streptococcus pneumoniae R6	55.556	100	0.557
comB	Streptococcus pneumoniae D39	55.556	100	0.557
comB	Streptococcus pneumoniae Rx1	55.556	100	0.557
comB	Streptococcus pneumoniae TIGR4	55.111	100	0.552
comB	Streptococcus mitis SK321	55.111	100	0.552

References

• [1]: Nicholas C K Heng et al. (2007) Competence-dependent bacteriocin production by Streptococcus gordonii DL1 (Challis). Journal of Bacteriology 189(4):1468-72. [PMID: 17012395]