Detailed information    

experimental Experimentally validated

Overview

Name   lytF   Type   Regulator
Locus tag   SGO_RS10250 Genome accession   NC_009785
Coordinates   2155996..2157645 (+) Length   549 a.a.
NCBI ID   WP_012131057.1    Uniprot ID   -
Organism   Streptococcus gordonii str. Challis substr. CH1     
Function   cell lysis   
Cell lysis

Function

In the present study we show that the genomes of nearly all species lacking CbpD encode an unrelated competence-regulated murein hydrolase termed LytF. Using Streptococcus gordonii as a model system, we obtained evidence indicating that LytF is a functional analogue of CbpD.


Genomic Context

Location: 2150996..2162645
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SGO_RS10220 (SGO_2087) - 2151141..2151368 (-) 228 WP_012131050.1 hypothetical protein -
  SGO_RS10225 (SGO_2088) - 2151388..2151651 (-) 264 WP_125367710.1 hypothetical protein -
  SGO_RS10230 (SGO_2089) purK 2151653..2152744 (-) 1092 WP_012131052.1 5-(carboxyamino)imidazole ribonucleotide synthase -
  SGO_RS10235 (SGO_2090) purE 2152731..2153219 (-) 489 WP_012131053.1 5-(carboxyamino)imidazole ribonucleotide mutase -
  SGO_RS10240 (SGO_2091) - 2153239..2154432 (-) 1194 WP_012131054.1 ATP-grasp domain-containing protein -
  SGO_RS10245 (SGO_2092) purD 2154492..2155751 (-) 1260 WP_012131055.1 phosphoribosylamine--glycine ligase -
  SGO_RS10250 (SGO_2094) lytF 2155996..2157645 (+) 1650 WP_012131057.1 GBS Bsp-like repeat-containing protein Regulator
  SGO_RS10255 (SGO_2096) comB 2157764..2159122 (-) 1359 WP_012131059.1 competence pheromone export protein ComB Regulator
  SGO_RS10260 (SGO_2097) comA 2159132..2161285 (-) 2154 WP_012131060.1 peptide cleavage/export ABC transporter ComA Regulator
  SGO_RS10265 (SGO_2098) rpsD 2161739..2162350 (-) 612 WP_008810013.1 30S ribosomal protein S4 -

Sequence

Protein


Download         Length: 549 a.a.        Molecular weight: 60540.38 Da        Isoelectric Point: 9.1621

>NTDB_id=426 SGO_RS10250 WP_012131057.1 2155996..2157645(+) (lytF) [Streptococcus gordonii str. Challis substr. CH1]
MKKYKNFLLLSATLLTSLSAPLAAANEQFSPSQLTAMTSEVYVQLEGQTAIVQYQPTAAQEAYTIQHAVWSEEGGQDDIV
WYSADQSTTRIDLSQHKGQGKFLIETYLNVNSVKYHLSSKSVFLNKPVQSTEKTVPEQQDSTSSNNATPQAPAEPKPTNP
APTPAPAPATNRVENPRNSYPKVEERNKSLPPQKVAQPKVAPPQAEPNNNKPLQASAARVPAEPVRDSKPLISITNINKE
NGTYTVRVQENPYSKKIKSVQVPIWSTANQSNLKWYEASKIGNGSFSVQFDIRNHQQIYGNYINHVYITYEDGSRVGYVA
ENVNLPQPAPQPLTPSLQVVHKGQSTYQVILQNSFGDGHILFPIWSDINGQDDLRWYTANSLGNGRYSLDFNANNHIGDG
LYHVHVYRQSGGKVTGVMASNFQVTRPTVAVRKPLNHTTDPSNTYPVGECTWGAKALAPWAGNWWGNGGDWAASARRAGF
RVGSTPQVGAIACWTDGGYGHVGVVTHVDSHKRIQIQESNYAGQRYIANFRGWFDPTIAQGTVSYIYPN

Nucleotide


Download         Length: 1650 bp        

>NTDB_id=426 SGO_RS10250 WP_012131057.1 2155996..2157645(+) (lytF) [Streptococcus gordonii str. Challis substr. CH1]
ATGAAAAAATACAAAAATTTCTTACTGCTTTCAGCAACCTTGTTGACCTCACTATCTGCTCCATTAGCTGCAGCGAATGA
ACAGTTTAGCCCATCCCAACTGACGGCTATGACCAGCGAAGTTTACGTTCAGCTTGAAGGACAAACAGCGATTGTTCAGT
ACCAACCGACCGCTGCGCAAGAAGCTTATACCATCCAACATGCCGTCTGGTCTGAGGAGGGAGGCCAAGACGATATTGTC
TGGTATTCAGCTGATCAAAGCACGACCAGGATTGACTTGTCTCAACACAAGGGCCAGGGTAAATTTCTCATCGAGACTTA
TCTCAATGTGAATAGTGTCAAATATCACTTGAGTAGTAAATCGGTCTTTCTAAACAAGCCGGTTCAATCAACCGAAAAAA
CTGTCCCTGAACAGCAAGACAGCACTTCGTCTAACAATGCCACACCTCAAGCACCGGCTGAGCCAAAACCAACTAACCCA
GCACCTACTCCAGCTCCGGCTCCCGCGACCAATAGAGTTGAAAATCCTCGGAACTCCTATCCAAAAGTCGAAGAGCGAAA
CAAATCTCTTCCTCCTCAAAAGGTAGCTCAACCAAAGGTGGCGCCCCCTCAGGCAGAGCCAAACAACAACAAACCTCTGC
AAGCTTCAGCTGCTAGAGTACCGGCAGAGCCTGTCAGAGACAGCAAGCCACTCATCAGTATTACCAACATTAATAAGGAA
AATGGAACCTATACCGTAAGGGTCCAAGAAAACCCTTATTCCAAGAAAATTAAAAGTGTGCAGGTGCCTATTTGGAGCAC
CGCAAATCAAAGCAATCTTAAATGGTATGAGGCTTCAAAGATAGGTAACGGAAGCTTTTCTGTTCAATTCGACATCCGAA
ATCACCAGCAGATTTACGGCAACTATATCAATCACGTCTACATTACCTATGAAGACGGTAGCCGAGTTGGCTATGTTGCT
GAAAATGTCAATCTGCCTCAGCCAGCACCCCAACCACTAACTCCGAGTCTGCAAGTGGTTCATAAAGGCCAATCGACCTA
TCAAGTTATCTTACAAAATAGTTTCGGTGACGGCCATATCCTCTTCCCCATCTGGTCAGATATTAATGGCCAAGACGACC
TCCGCTGGTATACAGCTAATTCTCTTGGTAATGGGCGCTATTCGTTAGATTTCAACGCCAACAATCATATCGGTGACGGT
CTCTATCATGTCCATGTCTATCGACAATCTGGCGGGAAAGTAACGGGTGTCATGGCCTCTAATTTTCAAGTAACACGCCC
AACGGTAGCTGTGAGAAAACCTCTTAACCATACGACCGACCCAAGTAATACTTACCCTGTTGGTGAATGCACATGGGGTG
CCAAGGCTCTCGCTCCTTGGGCAGGAAACTGGTGGGGAAATGGTGGGGACTGGGCAGCCAGTGCTCGCCGAGCAGGATTC
CGTGTAGGATCCACACCTCAGGTCGGAGCCATTGCCTGCTGGACTGACGGTGGATACGGACATGTCGGCGTCGTCACGCA
TGTTGATTCACATAAGCGTATCCAAATTCAGGAATCCAACTACGCTGGACAGCGCTATATCGCTAATTTCCGTGGCTGGT
TTGACCCAACCATCGCTCAGGGCACGGTTTCCTATATCTACCCAAATTAA

Domains

Predicted by InterproScan.

CHAP domain (CHAP)

(443-520)

GBS Bsp-like repeat (GBS_Bsp-like)

(342-414)

GBS Bsp-like repeat (GBS_Bsp-like)

(234-319)

GBS Bsp-like repeat (GBS_Bsp-like)

(53-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  lytF Streptococcus gordonii strain NCTC7865

95.446

100

0.954

Multiple sequence alignment    



References

[1] Kari Helene Berg et al. (2012) LytF, a novel competence-regulated murein hydrolase in the genus Streptococcus. Journal of Bacteriology 194(3):627-35. [PMID: 22123253]