Detailed information    

experimental Experimentally validated

Overview

Name   lytF   Type   Regulator
Locus tag   DQL23_RS10480 Genome accession   NZ_LS483341
Coordinates   2146781..2148430 (+) Length   549 a.a.
NCBI ID   WP_060554269.1    Uniprot ID   -
Organism   Streptococcus gordonii strain NCTC7865     
Function   cell lysis   
Cell lysis

Function

In the present study we show that the genomes of nearly all species lacking CbpD encode an unrelated competence-regulated murein hydrolase termed LytF. Using Streptococcus gordonii as a model system, we obtained evidence indicating that LytF is a functional analogue of CbpD.


Genomic Context

Location: 2141781..2153430
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQL23_RS10450 (NCTC7865_02108) - 2141822..2142049 (-) 228 WP_060554265.1 hypothetical protein -
  DQL23_RS10455 (NCTC7865_02109) - 2142069..2142335 (-) 267 WP_111705913.1 hypothetical protein -
  DQL23_RS10460 (NCTC7865_02110) purK 2142456..2143535 (-) 1080 WP_060554266.1 5-(carboxyamino)imidazole ribonucleotide synthase -
  DQL23_RS10465 (NCTC7865_02111) purE 2143522..2144010 (-) 489 WP_060554267.1 5-(carboxyamino)imidazole ribonucleotide mutase -
  DQL23_RS10470 (NCTC7865_02112) - 2144030..2145223 (-) 1194 WP_045504743.1 ATP-grasp domain-containing protein -
  DQL23_RS10475 (NCTC7865_02113) purD 2145278..2146537 (-) 1260 WP_060554268.1 phosphoribosylamine--glycine ligase -
  DQL23_RS10480 (NCTC7865_02114) lytF 2146781..2148430 (+) 1650 WP_060554269.1 GBS Bsp-like repeat-containing protein Regulator
  DQL23_RS10485 (NCTC7865_02115) comB 2148547..2149905 (-) 1359 WP_060554270.1 competence pheromone export protein ComB Regulator
  DQL23_RS10490 (NCTC7865_02116) comA 2149915..2152068 (-) 2154 WP_060554271.1 peptide cleavage/export ABC transporter ComA Regulator
  DQL23_RS10500 (NCTC7865_02117) rpsD 2152523..2153134 (-) 612 WP_008810013.1 30S ribosomal protein S4 -

Sequence

Protein


Download         Length: 549 a.a.        Molecular weight: 60493.21 Da        Isoelectric Point: 9.4781

>NTDB_id=427 DQL23_RS10480 WP_060554269.1 2146781..2148430(+) (lytF) [Streptococcus gordonii strain NCTC7865]
MKKYKNFLLLSATLLTSLSAPLAAANEQFSPSQLTAMTSEVSVQLEGQTAIVQYQPTTAQEAYPIQHAVWSEEGGQDDIV
WYSANQSTTRIDLSQHKGQGKFHIETYLNVNGVKYHLSSKSVFLNKPVQSAEKTVPEQQNSTSSNNATPQAPAEQKPTNP
APSPAPAPATNRVENRRNSYQKVEDRNKSLPPQKVAQPKATPPQAKPSNNKPLQASAARVPAEPVRDSKPLISITNINKE
NGTYTVRVQESPYSKRIKSVQVPIWSTANQSNLKWYEASQIGNGSFSVQFDIRNHQQLYGDYINHVYITYEDGSRAGYVA
ENVNLPQPAPQPLTPSLQVVHKGQSTYQVILQNSFGDGHILFPIWSDINGQDDLRWYTANSLGNGRYSLDFNANNHIGDG
LYHVHVYRQSGGKVTGVMASNFQVTRPTVTVRKPLNHATDPSNTYPVGECTWGAKALAPWAGNWWGNGGDWAASARRAGF
RVGSTPQVGAIACWTDGGYGHVGVVTHVDSHKRIQIQESNYAGQRYIANFRGWFDPTIAQGTVSYIYPN

Nucleotide


Download         Length: 1650 bp        

>NTDB_id=427 DQL23_RS10480 WP_060554269.1 2146781..2148430(+) (lytF) [Streptococcus gordonii strain NCTC7865]
ATGAAAAAATACAAAAATTTCTTACTGCTTTCAGCAACCTTGCTGACCTCACTATCCGCTCCATTAGCTGCAGCGAATGA
ACAGTTTAGCCCATCCCAGCTGACGGCTATGACCAGCGAAGTTTCCGTTCAGCTTGAAGGACAAACAGCGATTGTTCAGT
ATCAACCGACCACTGCGCAAGAAGCTTACCCCATCCAACATGCCGTCTGGTCTGAGGAGGGTGGCCAAGACGATATTGTC
TGGTATTCAGCTAATCAAAGCACGACCAGGATTGACTTGTCTCAGCACAAGGGCCAGGGCAAATTTCACATCGAGACTTA
TCTCAATGTAAATGGTGTCAAATATCACTTGAGTAGTAAATCGGTCTTTCTAAACAAGCCGGTTCAATCAGCCGAAAAAA
CTGTCCCTGAACAACAAAACAGCACTTCGTCTAACAATGCTACACCTCAAGCGCCTGCTGAGCAAAAGCCAACTAATCCA
GCACCTTCTCCAGCTCCGGCCCCCGCGACCAATAGAGTTGAAAACCGTCGGAACTCCTATCAAAAAGTCGAAGATCGAAA
CAAATCTCTTCCTCCTCAAAAGGTAGCTCAACCAAAGGCGACACCCCCTCAGGCAAAGCCAAGCAATAACAAACCTCTGC
AAGCTTCAGCTGCTAGAGTACCAGCAGAGCCTGTCAGAGACAGCAAGCCACTCATCAGTATTACCAACATTAATAAGGAA
AATGGAACCTATACCGTAAGGGTCCAAGAAAGTCCTTATTCTAAGAGAATAAAAAGTGTGCAGGTGCCTATTTGGAGTAC
TGCTAATCAAAGCAATCTTAAATGGTATGAGGCTTCACAAATAGGCAACGGAAGCTTCTCCGTTCAATTCGATATCCGAA
ATCACCAGCAGCTTTACGGCGACTATATCAATCACGTCTACATTACCTATGAAGACGGTAGCCGAGCTGGCTATGTTGCT
GAAAATGTCAATCTGCCTCAGCCAGCACCCCAACCACTAACTCCGAGTCTGCAAGTGGTCCATAAAGGCCAATCGACCTA
TCAAGTTATCTTACAAAATAGTTTCGGTGACGGCCATATTCTCTTCCCCATCTGGTCAGATATTAATGGCCAAGACGACC
TCCGCTGGTATACAGCTAATTCTCTTGGTAATGGGCGCTATTCGTTAGATTTCAACGCCAACAATCATATCGGTGACGGT
CTCTATCATGTCCATGTCTATCGACAATCTGGTGGGAAAGTAACGGGTGTCATGGCCTCTAATTTTCAAGTGACACGCCC
AACGGTAACTGTGAGAAAACCTCTTAACCATGCAACCGACCCAAGTAACACCTACCCTGTTGGTGAATGTACATGGGGTG
CCAAGGCTCTCGCTCCTTGGGCAGGAAACTGGTGGGGAAATGGTGGGGACTGGGCAGCCAGTGCTCGCCGAGCAGGATTC
CGTGTAGGATCCACACCTCAGGTCGGAGCCATTGCCTGCTGGACTGACGGTGGATACGGACATGTCGGCGTCGTCACGCA
TGTTGATTCACATAAGCGTATCCAAATTCAGGAATCCAACTACGCTGGACAGCGCTATATCGCTAATTTCCGTGGCTGGT
TTGACCCAACCATCGCTCAGGGCACGGTTTCCTATATCTACCCAAATTAA

Domains

Predicted by InterproScan.

GBS Bsp-like repeat (GBS_Bsp-like)

(342-414)

GBS Bsp-like repeat (GBS_Bsp-like)

(59-113)

GBS Bsp-like repeat (GBS_Bsp-like)

(234-319)

CHAP domain (CHAP)

(443-520)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  lytF Streptococcus gordonii str. Challis substr. CH1

95.446

100

0.954

Multiple sequence alignment    



References

[1] Kari Helene Berg et al. (2012) LytF, a novel competence-regulated murein hydrolase in the genus Streptococcus. Journal of Bacteriology 194(3):627-35. [PMID: 22123253]