Detailed information NTG0000132

Experimentally validated

Overview

• Name: comB
• Type: Regulator
• Locus tag: SP_RS00260
• Genome accession: NC_003028
• Coordinates: 42982..44331 (+)
• Length: 449 a.a.
• NCBI ID: WP_000801611.1
• Uniprot ID: -
• Organism: Streptococcus pneumoniae TIGR4
• Function: transport of ComC Competence regulation

Function

ComAB transporter secret the mature competence-stimulating peptide (CSP) into the extracellular environment. This secretion is a critical step in the quorum-sensing mechanism that triggers competence development. Once CSP reaches a threshold concentration, it activates the ComDE two-component system, leading to the expression of early competence genes and eventually the late competence genes required for DNA uptake and recombination.

Genomic Context

Location: 37982..49331

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SP_RS00225 (SP_0037)	plsX	38101..39093 (+)	993	WP_000717458.1	phosphate acyltransferase PlsX	-
SP_RS00230 (SP_0038)	-	39099..39332 (+)	234	WP_000136447.1	acyl carrier protein	-
SP_RS00235 (SP_0039)	-	39369..39668 (+)	300	Protein_35	transposase family protein	-
SP_RS00240 (SP_0041)	blpU	39871..40101 (+)	231	WP_001093487.1	bacteriocin-like peptide BlpU	-
SP_RS13575	-	40104..40229 (+)	126	WP_000346297.1	PncF family bacteriocin immunity protein	-
SP_RS00255 (SP_0042)	comA	40816..42969 (+)	2154	WP_000668284.1	peptide cleavage/export ABC transporter ComA	Regulator
SP_RS00260 (SP_0043)	comB	42982..44331 (+)	1350	WP_000801611.1	competence pheromone export protein ComB	Regulator
SP_RS00265 (SP_0044)	purC	44501..45208 (+)	708	WP_000043309.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
SP_RS13705	-	45210..45353 (+)	144	WP_050167432.1	hypothetical protein	-
SP_RS00270 (SP_0045)	-	45410..49135 (+)	3726	WP_000361188.1	phosphoribosylformylglycinamidine synthase	-

Regulatory network

Positive effect      

Negative effect

Regulator	Target	Regulation
comB	comC/comC2	positive effect
comC/comC2	comD/comD2	positive effect
comD/comD2	comE	positive effect
comE	comE	positive effect
comE	comX/comX1	positive effect
comE	comW	positive effect
comE	comA	positive effect
comE	comB	positive effect
comE	comM	positive effect
comE	comX/comX2	positive effect
comE	comD/comD2	positive effect
comE	comC/comC2	positive effect
stkP	comE	positive effect
comX/comX1	late competence genes	positive effect
clpP	comX/comX1	negative effect
comW	comX/comX1	positive effect
clpE	comX/comX1	negative effect
comW	comX/comX2	positive effect
clpP	comW	negative effect
mecA	comW	negative effect
clpC	comW	negative effect
comA	comC/comC2	positive effect
comM	cbpD	negative effect
comX/comX2	late competence genes	positive effect
clpP	comX/comX2	negative effect
clpE	comX/comX2	negative effect
ciaH	comC/comC2	negative effect
htrA	comC/comC2	negative effect
ciaR	comC/comC2	negative effect
comX/comX2	late competence genes	positive effect
comX/comX1	late competence genes	positive effect
cbpD	lytA	positive effect
cbpD	lytC	positive effect
ciaH	htrA	positive effect
htrA	comEC/celB	negative effect
htrA	comEA/celA/cilE	negative effect
ciaR	htrA	positive effect

Sequence

Protein

• Length: 449 a.a.
• Molecular weight: 49601.49 Da
• Isoelectric Point: 5.5642

>NTDB_id=132 SP_RS00260 WP_000801611.1 42982..44331(+) (comB) [Streptococcus pneumoniae TIGR4]
MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKEMSLSTRATVEPSRILANIQSTSNNRILVNHLEENKL
VKKGDLLVQYQEGAEGVQAESYASQLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQN
ETIASQNAAASQTQAEIGNLISQTEAKIRDYQTAKSAIETGASLAGQNLAYSLYQSYKSQGEENPQTKVQAVAQVEAQIS
QLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQHLAKVGQELTLLAQKILEAESGKKVQGNLLDKGKVTASEDGVLH
LNPETSDSSMVAEGALLAQLYPSLEREGKAKLTAYLSSKYVARIKVGDSVRYTTTHDAGNQLFLDSTITSIDATATKTEK
GNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE

Nucleotide

• Length: 1350 bp

>NTDB_id=132 SP_RS00260 WP_000801611.1 42982..44331(+) (comB) [Streptococcus pneumoniae TIGR4]
ATGAAACCAGAATTTTTAGAAAGTGCGGAGTTTTATAATCGTCGTTACCATAATTTTTCCAGTAGTGTGATTGTACCCAT
GGCCCTTCTGCTTGTGTTTTTACTTGGCTTTGCAACTGTTGCAGAGAAGGAGATGAGTTTGTCCACTAGAGCTACTGTCG
AACCTAGTCGTATCCTTGCAAATATCCAGTCAACTAGCAACAATCGTATTCTTGTCAATCATTTGGAAGAAAATAAGCTG
GTTAAGAAGGGGGATCTTTTGGTTCAATACCAAGAAGGGGCAGAGGGTGTCCAAGCGGAGTCCTATGCCAGTCAGTTGGA
CATGCTAAAGGATCAAAAAAAGCAATTGGAGTATCTGCAAAAGAGCCTGCAAGAAGGGGAGAACCACTTTCCAGAGGAGG
ATAAGTTTGGCTACCAAGCCACCTTTCGCGACTACATCAGTCAAGCAGGCAGTCTTAGGGCTAGTACATCGCAACAAAAT
GAGACCATCGCGTCCCAGAATGCAGCAGCTAGCCAAACCCAAGCCGAAATCGGCAACCTCATCAGTCAAACAGAGGCTAA
AATTCGCGATTACCAGACAGCTAAGTCAGCTATTGAAACAGGTGCTTCCTTGGCCGGTCAGAATCTAGCCTACTCTCTTT
ACCAGTCCTACAAGTCTCAGGGCGAGGAAAATCCCCAAACTAAGGTTCAGGCAGTTGCACAGGTTGAAGCACAGATTTCT
CAGTTAGAATCTAGTCTTGCTACTTACCGTGTCCAGTATGCAGGTTCAGGTACCCAGCAAGCCTATGCGTCAGGGTTAAG
CAGTCAATTGGAATCCCTTAAATCCCAACACTTGGCAAAGGTTGGTCAGGAATTGACCCTTCTAGCCCAGAAAATTTTGG
AGGCAGAGTCAGGTAAGAAGGTACAGGGAAATCTTTTAGACAAGGGGAAAGTTACGGCGAGTGAGGATGGGGTGCTTCAT
CTTAATCCTGAGACCAGTGATTCTAGCATGGTTGCAGAAGGTGCCCTACTAGCCCAACTTTATCCATCTTTGGAAAGAGA
AGGGAAAGCCAAACTCACAGCTTATCTAAGTTCAAAATATGTAGCAAGAATCAAGGTCGGTGATTCTGTTCGCTATACTA
CGACTCATGATGCCGGGAATCAACTTTTCCTAGATTCTACTATTACAAGTATTGATGCGACAGCTACTAAGACTGAGAAA
GGGAATTTCTTTAAAATCGAGGCGGAGACTAATCTAACTTCGGAGCAGGCTGAAAAACTTAGGTACGGGGTGGAAGGCCG
CTTGCAGATGATTACGGGCAAGAAAAGTTACCTACGTTATTATTTGGATCAATTTTTGAACAAAGAGTAA

Domains

No domain identified.

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comB	Streptococcus pneumoniae Rx1	98.886	100	0.989
comB	Streptococcus pneumoniae D39	98.886	100	0.989
comB	Streptococcus pneumoniae R6	98.886	100	0.989
comB	Streptococcus mitis SK321	94.655	100	0.947
comB	Streptococcus mitis NCTC 12261	94.209	100	0.942
comB	Streptococcus gordonii str. Challis substr. CH1	55.111	100	0.552

References

• [1]: L S Håvarstein et al. (1995) An unmodified heptadecapeptide pheromone induces competence for genetic transformation in Streptococcus pneumoniae. Proceedings of The National Academy of Sciences of The United States of America 92(24):11140-4. [PMID: 7479953]
• [2]: L S Håvarstein et al. (1995) A family of bacteriocin ABC transporters carry out proteolytic processing of their substrates concomitant with export. Molecular Microbiology 16(2):229-40. [PMID: 7565085]
• [3]: F M Hui et al. (1991) Genetic transformation in Streptococcus pneumoniae: nucleotide sequence analysis shows comA, a gene required for competence induction, to be a member of the bacterial ATP-dependent transport protein family. Journal of Bacteriology 173(1):372-81. [PMID: 1987129]