Detailed information    

experimental Experimentally validated

Overview

Name   mecA   Type   Regulator
Locus tag   SP_RS06670 Genome accession   NC_003028
Coordinates   1284534..1285271 (-) Length   245 a.a.
NCBI ID   WP_000782668.1    Uniprot ID   Q97Q68
Organism   Streptococcus pneumoniae TIGR4     
Function   degradation of ComW   
Competence regulation

Function

ComW is a target of the Clp machinery and was shown to be degraded by ClpCP. This degradation is likely to require the adaptor protein MecA, an obligate partner of ClpC, as MecA inactivation leads to ComW stabilisation.


Genomic Context

Location: 1279534..1290271
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SP_RS06650 (SP_1359) msrB 1280993..1281931 (-) 939 WP_000818207.1 peptide-methionine (R)-S-oxide reductase MsrB -
  SP_RS13420 - 1281998..1282150 (-) 153 WP_239424733.1 chlorohydrolase -
  SP_RS06660 (SP_1360) thrB 1282226..1283095 (-) 870 WP_000692438.1 homoserine kinase -
  SP_RS06665 (SP_1361) - 1283097..1284383 (-) 1287 WP_000216382.1 homoserine dehydrogenase -
  SP_RS06670 (SP_1362) mecA 1284534..1285271 (-) 738 WP_000782668.1 adaptor protein MecA Regulator
  SP_RS06675 (SP_1363) - 1285471..1286505 (-) 1035 WP_000461505.1 hypothetical protein -
  SP_RS06680 (SP_1364) - 1286534..1287967 (-) 1434 WP_000565078.1 O-antigen polysaccharide polymerase Wzy family protein -
  SP_RS06685 (SP_1365) - 1287983..1288969 (-) 987 WP_000446975.1 glycosyltransferase family 2 protein -
  SP_RS06690 (SP_1366) - 1288971..1290074 (-) 1104 WP_000225907.1 glycosyltransferase -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  mecA comW negative effect
  comW comX/comX1 positive effect
  comX/comX1 late competence genes positive effect
  comX/comX2 late competence genes positive effect
  comX/comX2 late competence genes positive effect
  comE comX/comX2 positive effect
  clpP comX/comX2 negative effect
  comW comX/comX2 positive effect
  clpE comX/comX2 negative effect
  comX/comX1 late competence genes positive effect
  comE comX/comX1 positive effect
  clpP comX/comX1 negative effect
  clpE comX/comX1 negative effect
  comE comE positive effect
  comE comW positive effect
  comE comA positive effect
  comE comB positive effect
  comE comM positive effect
  comE comD/comD2 positive effect
  comE comC/comC2 positive effect
  comD/comD2 comE positive effect
  stkP comE positive effect
  clpP comW negative effect
  clpC comW negative effect
  comA comC/comC2 positive effect
  comB comC/comC2 positive effect
  comM cbpD negative effect
  comC/comC2 comD/comD2 positive effect
  ciaH comC/comC2 negative effect
  htrA comC/comC2 negative effect
  ciaR comC/comC2 negative effect
  cbpD lytA positive effect
  cbpD lytC positive effect
  ciaH htrA positive effect
  htrA comEC/celB negative effect
  htrA comEA/celA/cilE negative effect
  ciaR htrA positive effect

Sequence

Protein


Download         Length: 245 a.a.        Molecular weight: 28391.20 Da        Isoelectric Point: 4.0199

>NTDB_id=283 SP_RS06670 WP_000782668.1 1284534..1285271(-) (mecA) [Streptococcus pneumoniae TIGR4]
MKMKQISDTTLKITISLEDLMDRGMEIADFLVPQEKTEEFFYAILDELEMPDSFLDTGMLSFRVTPKPDKVDVFVTKSKI
DQNLDFEDLSDLPDMEELAQMSPDEFIKTLEKSIADKTKDDIEAIQSLEQVEVKEEEQEQAEQEAESKKEPYIYYILSFA
KLADLVAFAKTVTFEMETSELYKMNERYYLTILVDIENHPSPYPAWLLARMREFADDSDISRSVLQEYGQVLMSHDAVLN
LQKIG

Nucleotide


Download         Length: 738 bp        

>NTDB_id=283 SP_RS06670 WP_000782668.1 1284534..1285271(-) (mecA) [Streptococcus pneumoniae TIGR4]
ATGAAAATGAAACAAATTAGTGATACAACTTTAAAAATCACGATATCTTTAGAGGATTTGATGGATCGTGGAATGGAGAT
TGCTGACTTTCTCGTTCCTCAAGAAAAAACAGAAGAGTTCTTTTATGCTATCTTGGATGAGCTAGAGATGCCTGATAGCT
TTCTGGATACAGGCATGTTGAGCTTCCGTGTGACTCCAAAACCTGATAAGGTAGATGTCTTTGTGACCAAGTCAAAGATT
GACCAAAATCTAGATTTTGAAGACTTATCGGATTTGCCAGATATGGAAGAATTGGCTCAAATGTCTCCAGATGAATTTAT
CAAAACCCTGGAAAAAAGCATCGCAGACAAAACCAAGGATGATATCGAAGCCATTCAATCTCTTGAGCAAGTTGAAGTCA
AGGAAGAAGAGCAAGAGCAGGCTGAACAAGAAGCTGAGAGTAAGAAAGAACCTTACATCTACTACATCCTTTCTTTTGCT
AAGTTGGCTGACTTGGTAGCTTTTGCCAAGACAGTGACTTTTGAAATGGAAACTTCTGAACTCTACAAAATGAACGAGCG
CTATTATTTGACCATTTTAGTGGATATTGAAAATCATCCAAGCCCATATCCAGCTTGGCTGTTGGCCCGTATGCGCGAGT
TTGCAGACGATAGTGATATCAGTCGCTCAGTCTTACAAGAGTATGGTCAAGTCTTGATGAGTCACGATGCAGTGCTCAAT
CTGCAAAAAATCGGCTAA

Domains

Predicted by InterproScan.

Negative regulator of genetic competence (MecA) (MecA)

(1-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q97Q68

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  mecA Streptococcus pneumoniae Rx1

99.592

100

0.996
  mecA Streptococcus pneumoniae D39

99.592

100

0.996
  mecA Streptococcus pneumoniae R6

99.592

100

0.996
  mecA Streptococcus mutans UA159

48.571

100

0.496
  mecA Streptococcus thermophilus LMD-9

48.594

100

0.494
  mecA Streptococcus thermophilus LMG 18311

48.193

100

0.49

Multiple sequence alignment    



References

[1] Laetitia Fontaine et al. (2015) Regulation of competence for natural transformation in streptococci. Infection, Genetics And Evolution : Journal of Molecular Epidemiology And Evolutionary Genetics in Infectious Diseases 33:343-60. [PMID: 25236918]